Atrophic meibomian gland dysfunction induced by eyelid margin cryotherapy with liquid nitrogen
Abstract To develop an atrophic Meibomian Gland Dysfunction (MGD) animal model via liquid nitrogen cryotherapy, the eyelid edges of C57 mice exposure to liquid nitrogen for 30 s. Morphology of MG and ocular surface were assessed using stereomicroscopy and a slit lamp microscope at multiple time poin...
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| Format: | Article |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-84742-0 |
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| author | Shu Wang Yulin Li Jingfan Gao Jia Lin Xin Jin Hong Zhang |
| author_facet | Shu Wang Yulin Li Jingfan Gao Jia Lin Xin Jin Hong Zhang |
| author_sort | Shu Wang |
| collection | DOAJ |
| description | Abstract To develop an atrophic Meibomian Gland Dysfunction (MGD) animal model via liquid nitrogen cryotherapy, the eyelid edges of C57 mice exposure to liquid nitrogen for 30 s. Morphology of MG and ocular surface were assessed using stereomicroscopy and a slit lamp microscope at multiple time points post-injury. Acinar loss and atrophy were observed from day 7, with increased inflammation and apoptosis, and decreased proliferation in acinar cells. Corneal epithelial defects appeared after day 14. Liquid nitrogen induced selective damage to meibomian acinar cells, simulating MGD pathology effectively, with peak effects at day 21, providing a relevant model for atrophic MGD research. |
| format | Article |
| id | doaj-art-52538ad4e50043378b591a4011d39364 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-52538ad4e50043378b591a4011d393642025-01-05T12:23:29ZengNature PortfolioScientific Reports2045-23222025-01-0115111510.1038/s41598-024-84742-0Atrophic meibomian gland dysfunction induced by eyelid margin cryotherapy with liquid nitrogenShu Wang0Yulin Li1Jingfan Gao2Jia Lin3Xin Jin4Hong Zhang5Eye Hospital, The First Affiliated Hospital of Harbin Medical UniversityEye Hospital, The First Affiliated Hospital of Harbin Medical UniversityEye Hospital, The First Affiliated Hospital of Harbin Medical UniversityEye Hospital, The First Affiliated Hospital of Harbin Medical UniversityEye Hospital, The First Affiliated Hospital of Harbin Medical UniversityEye Hospital, The First Affiliated Hospital of Harbin Medical UniversityAbstract To develop an atrophic Meibomian Gland Dysfunction (MGD) animal model via liquid nitrogen cryotherapy, the eyelid edges of C57 mice exposure to liquid nitrogen for 30 s. Morphology of MG and ocular surface were assessed using stereomicroscopy and a slit lamp microscope at multiple time points post-injury. Acinar loss and atrophy were observed from day 7, with increased inflammation and apoptosis, and decreased proliferation in acinar cells. Corneal epithelial defects appeared after day 14. Liquid nitrogen induced selective damage to meibomian acinar cells, simulating MGD pathology effectively, with peak effects at day 21, providing a relevant model for atrophic MGD research.https://doi.org/10.1038/s41598-024-84742-0Meibomian gland dysfunctionLiquid nitrogenAcinar atrophyModel |
| spellingShingle | Shu Wang Yulin Li Jingfan Gao Jia Lin Xin Jin Hong Zhang Atrophic meibomian gland dysfunction induced by eyelid margin cryotherapy with liquid nitrogen Scientific Reports Meibomian gland dysfunction Liquid nitrogen Acinar atrophy Model |
| title | Atrophic meibomian gland dysfunction induced by eyelid margin cryotherapy with liquid nitrogen |
| title_full | Atrophic meibomian gland dysfunction induced by eyelid margin cryotherapy with liquid nitrogen |
| title_fullStr | Atrophic meibomian gland dysfunction induced by eyelid margin cryotherapy with liquid nitrogen |
| title_full_unstemmed | Atrophic meibomian gland dysfunction induced by eyelid margin cryotherapy with liquid nitrogen |
| title_short | Atrophic meibomian gland dysfunction induced by eyelid margin cryotherapy with liquid nitrogen |
| title_sort | atrophic meibomian gland dysfunction induced by eyelid margin cryotherapy with liquid nitrogen |
| topic | Meibomian gland dysfunction Liquid nitrogen Acinar atrophy Model |
| url | https://doi.org/10.1038/s41598-024-84742-0 |
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