Pollen metabarcoding reveals a broad diversity of plant sources available to farmland flower visitors near tropical montane forest
Despite the widely recognized role of pollinators in ecosystem services, we currently have a poor understanding of the contribution of Natural Protected Areas neighboring agricultural landscapes to crop pollinator diversity and plant-pollinator interactions. Here, we conducted monthly surveys over a...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1472066/full |
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| author | B. Karina Montero B. Karina Montero Nicole Gamboa-Barrantes Nicole Gamboa-Barrantes Geovanna Rojas-Malavasi Geovanna Rojas-Malavasi E. Jacob Cristóbal-Perez E. Jacob Cristóbal-Perez Gilbert Barrantes Gilbert Barrantes Gilbert Barrantes Alfredo Cascante-Marín Alfredo Cascante-Marín Alfredo Cascante-Marín Paul Hanson Paul Hanson Manuel A. Zumbado Ruth Madrigal-Brenes Ruth Madrigal-Brenes Ruth Madrigal-Brenes Silvana Martén-Rodríguez Silvana Martén-Rodríguez Silvana Martén-Rodríguez Mauricio Quesada Mauricio Quesada Eric J. Fuchs Eric J. Fuchs Eric J. Fuchs Eric J. Fuchs |
| author_facet | B. Karina Montero B. Karina Montero Nicole Gamboa-Barrantes Nicole Gamboa-Barrantes Geovanna Rojas-Malavasi Geovanna Rojas-Malavasi E. Jacob Cristóbal-Perez E. Jacob Cristóbal-Perez Gilbert Barrantes Gilbert Barrantes Gilbert Barrantes Alfredo Cascante-Marín Alfredo Cascante-Marín Alfredo Cascante-Marín Paul Hanson Paul Hanson Manuel A. Zumbado Ruth Madrigal-Brenes Ruth Madrigal-Brenes Ruth Madrigal-Brenes Silvana Martén-Rodríguez Silvana Martén-Rodríguez Silvana Martén-Rodríguez Mauricio Quesada Mauricio Quesada Eric J. Fuchs Eric J. Fuchs Eric J. Fuchs Eric J. Fuchs |
| author_sort | B. Karina Montero |
| collection | DOAJ |
| description | Despite the widely recognized role of pollinators in ecosystem services, we currently have a poor understanding of the contribution of Natural Protected Areas neighboring agricultural landscapes to crop pollinator diversity and plant-pollinator interactions. Here, we conducted monthly surveys over a period of one year to study the diversity of insect visitors in dominant fruit crops—avocado, plum, apple, and blackberry—and used pollen DNA metabarcoding to characterize the community of plant sources in and around low-intensive farmland bordered by protected montane forest in Costa Rica. We found that crops and native plants had distinct communities of flower visitors, suggesting the presence of fine-scale habitat differences. DNA metabarcoding coupled with a custom-built reference database, enabled us to identify plant sources among pollen samples with high taxonomic resolution (species or genus level). We found that insect visitors carried pollen from a large diversity of plant taxa, including species native to the montane forests and highland páramos of Costa Rica. The diversity and composition of plant sources were variable across fruit crops and insect groups. Wildflower visitors such as bumblebees and syrphid flies, use a diverse range of plant taxa at similar levels to managed honeybees. This indicates the potential contribution of a diverse community of insect visitors to the pollination services of fruit crops and native flora. Overall, our study suggests that low-intensive farming practices that promote the presence of common ruderals combined with nearby protected forests contribute to maintaining diverse insect communities that provide crucial pollination services. |
| format | Article |
| id | doaj-art-52285224ab024413bb948a0e66e10081 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-52285224ab024413bb948a0e66e100812025-01-07T04:13:42ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.14720661472066Pollen metabarcoding reveals a broad diversity of plant sources available to farmland flower visitors near tropical montane forestB. Karina Montero0B. Karina Montero1Nicole Gamboa-Barrantes2Nicole Gamboa-Barrantes3Geovanna Rojas-Malavasi4Geovanna Rojas-Malavasi5E. Jacob Cristóbal-Perez6E. Jacob Cristóbal-Perez7Gilbert Barrantes8Gilbert Barrantes9Gilbert Barrantes10Alfredo Cascante-Marín11Alfredo Cascante-Marín12Alfredo Cascante-Marín13Paul Hanson14Paul Hanson15Manuel A. Zumbado16Ruth Madrigal-Brenes17Ruth Madrigal-Brenes18Ruth Madrigal-Brenes19Silvana Martén-Rodríguez20Silvana Martén-Rodríguez21Silvana Martén-Rodríguez22Mauricio Quesada23Mauricio Quesada24Eric J. Fuchs25Eric J. Fuchs26Eric J. Fuchs27Eric J. Fuchs28Centro de Investigación en Biodiversidad y Ecología Tropical, Universidad de Costa Rica, San José, Costa RicaEscuela de Biología, Universidad de Costa Rica, San José, Costa RicaCentro de Investigación en Biodiversidad y Ecología Tropical, Universidad de Costa Rica, San José, Costa RicaEscuela de Biología, Universidad de Costa Rica, San José, Costa RicaCentro de Investigación en Biodiversidad y Ecología Tropical, Universidad de Costa Rica, San José, Costa RicaEscuela de Biología, Universidad de Costa Rica, San José, Costa RicaLaboratorio Nacional de Análisis y Síntesis Ecológica, Escuela Nacional de Estudios Superiores, Unidad Morelia, Universidad Nacional Autónoma de México, Morelia, Michoacán, MexicoLaboratorio Binacional de Análisis y Síntesis Ecológica, Universidad Nacional Autónoma de México - Universidad de Costa Rica (UNAM-UCR), Morelia, Michoacán, MexicoCentro de Investigación en Biodiversidad y Ecología Tropical, Universidad de Costa Rica, San José, Costa RicaEscuela de Biología, Universidad de Costa Rica, San José, Costa RicaLaboratorio Binacional de Análisis y Síntesis Ecológica, Universidad Nacional Autónoma de México - Universidad de Costa Rica (UNAM-UCR), Morelia, Michoacán, MexicoCentro de Investigación en Biodiversidad y Ecología Tropical, Universidad de Costa Rica, San José, Costa RicaEscuela de Biología, Universidad de Costa Rica, San José, Costa RicaLaboratorio Binacional de Análisis y Síntesis Ecológica, Universidad Nacional Autónoma de México - Universidad de Costa Rica (UNAM-UCR), Morelia, Michoacán, MexicoCentro de Investigación en Biodiversidad y Ecología Tropical, Universidad de Costa Rica, San José, Costa RicaEscuela de Biología, Universidad de Costa Rica, San José, Costa RicaInvestigador Colaborador, Museo de Zoología, Universidad de Costa Rica, San José, Costa RicaCentro de Investigación en Biodiversidad y Ecología Tropical, Universidad de Costa Rica, San José, Costa RicaEscuela de Biología, Universidad de Costa Rica, San José, Costa RicaLaboratorio Binacional de Análisis y Síntesis Ecológica, Universidad Nacional Autónoma de México - Universidad de Costa Rica (UNAM-UCR), Morelia, Michoacán, MexicoLaboratorio Nacional de Análisis y Síntesis Ecológica, Escuela Nacional de Estudios Superiores, Unidad Morelia, Universidad Nacional Autónoma de México, Morelia, Michoacán, MexicoLaboratorio Binacional de Análisis y Síntesis Ecológica, Universidad Nacional Autónoma de México - Universidad de Costa Rica (UNAM-UCR), Morelia, Michoacán, MexicoLaboratorio de Ecología Evolutiva de Plantas, Escuela Nacional de Estudios Superiores-Morelia, Universidad Nacional Autónoma de México, Morelia, Michoacán, MexicoLaboratorio Nacional de Análisis y Síntesis Ecológica, Escuela Nacional de Estudios Superiores, Unidad Morelia, Universidad Nacional Autónoma de México, Morelia, Michoacán, MexicoLaboratorio Binacional de Análisis y Síntesis Ecológica, Universidad Nacional Autónoma de México - Universidad de Costa Rica (UNAM-UCR), Morelia, Michoacán, MexicoCentro de Investigación en Biodiversidad y Ecología Tropical, Universidad de Costa Rica, San José, Costa RicaEscuela de Biología, Universidad de Costa Rica, San José, Costa RicaLaboratorio Nacional de Análisis y Síntesis Ecológica, Escuela Nacional de Estudios Superiores, Unidad Morelia, Universidad Nacional Autónoma de México, Morelia, Michoacán, MexicoLaboratorio Binacional de Análisis y Síntesis Ecológica, Universidad Nacional Autónoma de México - Universidad de Costa Rica (UNAM-UCR), Morelia, Michoacán, MexicoDespite the widely recognized role of pollinators in ecosystem services, we currently have a poor understanding of the contribution of Natural Protected Areas neighboring agricultural landscapes to crop pollinator diversity and plant-pollinator interactions. Here, we conducted monthly surveys over a period of one year to study the diversity of insect visitors in dominant fruit crops—avocado, plum, apple, and blackberry—and used pollen DNA metabarcoding to characterize the community of plant sources in and around low-intensive farmland bordered by protected montane forest in Costa Rica. We found that crops and native plants had distinct communities of flower visitors, suggesting the presence of fine-scale habitat differences. DNA metabarcoding coupled with a custom-built reference database, enabled us to identify plant sources among pollen samples with high taxonomic resolution (species or genus level). We found that insect visitors carried pollen from a large diversity of plant taxa, including species native to the montane forests and highland páramos of Costa Rica. The diversity and composition of plant sources were variable across fruit crops and insect groups. Wildflower visitors such as bumblebees and syrphid flies, use a diverse range of plant taxa at similar levels to managed honeybees. This indicates the potential contribution of a diverse community of insect visitors to the pollination services of fruit crops and native flora. Overall, our study suggests that low-intensive farming practices that promote the presence of common ruderals combined with nearby protected forests contribute to maintaining diverse insect communities that provide crucial pollination services.https://www.frontiersin.org/articles/10.3389/fpls.2024.1472066/fullinsect visitor communitieslow-intensive farmingfruit cropstropical montane forestecosystem services |
| spellingShingle | B. Karina Montero B. Karina Montero Nicole Gamboa-Barrantes Nicole Gamboa-Barrantes Geovanna Rojas-Malavasi Geovanna Rojas-Malavasi E. Jacob Cristóbal-Perez E. Jacob Cristóbal-Perez Gilbert Barrantes Gilbert Barrantes Gilbert Barrantes Alfredo Cascante-Marín Alfredo Cascante-Marín Alfredo Cascante-Marín Paul Hanson Paul Hanson Manuel A. Zumbado Ruth Madrigal-Brenes Ruth Madrigal-Brenes Ruth Madrigal-Brenes Silvana Martén-Rodríguez Silvana Martén-Rodríguez Silvana Martén-Rodríguez Mauricio Quesada Mauricio Quesada Eric J. Fuchs Eric J. Fuchs Eric J. Fuchs Eric J. Fuchs Pollen metabarcoding reveals a broad diversity of plant sources available to farmland flower visitors near tropical montane forest Frontiers in Plant Science insect visitor communities low-intensive farming fruit crops tropical montane forest ecosystem services |
| title | Pollen metabarcoding reveals a broad diversity of plant sources available to farmland flower visitors near tropical montane forest |
| title_full | Pollen metabarcoding reveals a broad diversity of plant sources available to farmland flower visitors near tropical montane forest |
| title_fullStr | Pollen metabarcoding reveals a broad diversity of plant sources available to farmland flower visitors near tropical montane forest |
| title_full_unstemmed | Pollen metabarcoding reveals a broad diversity of plant sources available to farmland flower visitors near tropical montane forest |
| title_short | Pollen metabarcoding reveals a broad diversity of plant sources available to farmland flower visitors near tropical montane forest |
| title_sort | pollen metabarcoding reveals a broad diversity of plant sources available to farmland flower visitors near tropical montane forest |
| topic | insect visitor communities low-intensive farming fruit crops tropical montane forest ecosystem services |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1472066/full |
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