Aurora 2.0: A fluorogenic dye library for expanding the capability of protein-adaptive differential scanning fluorimetry (paDSF)

Aurora 2.0: A fluorogenic dye library for expanding the capability of protein-adaptive differential scanning fluorimetry (paDSF)

Differential Scanning Fluorimetry (DSF) is a biophysical assay that is used to estimate protein stability in vitro. In a DSF experiment, the increased fluorescence of a solvatochromatic dye, such as Sypro Orange, is used to detect the unfolding of a protein during heating. However, Sypro Orange is o...

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Bibliographic Details
Main Authors: Annemarie F. Charvat, Kayleigh Mason-Chalmers, Aneta Grabinska-Rogala, Shloka Shivakumar, Zachary Gale-Day, Taiasean Wu, Zoe Millbern, Jonathan B. Grimm, Emma C. Carroll, K․ Peter R․ Nilsson, Luke D. Lavis, Nelson R. Vinueza, Jason E. Gestwicki
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:SLAS Discovery
Subjects:
Thermal shift assay
Thermofluor
Protein stability
Binding assay
High throughput screening
Fluorescent probes
Online Access:http://www.sciencedirect.com/science/article/pii/S2472555225000528
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