Determination of Photosensitizing Potential of Lapachol for Photodynamic Inactivation of Bacteria
Antimicrobial photodynamic inactivation (aPDI) offers a promising alternative to combat drug-resistant bacteria. This study explores the potential of lapachol, a natural naphthoquinone derived from <i>Tabebuia avellanedae</i>, as a photosensitizer (PS) for aPDI. Lapachol’s photosensitizi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/21/5184 |
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| author | Regiane G. Lima Raphael S. Flores Gabriella Miessi Jhoenne H. V. Pulcherio Laís F. Aguilera Leandro O. Araujo Samuel L. Oliveira Anderson R. L. Caires |
| author_facet | Regiane G. Lima Raphael S. Flores Gabriella Miessi Jhoenne H. V. Pulcherio Laís F. Aguilera Leandro O. Araujo Samuel L. Oliveira Anderson R. L. Caires |
| author_sort | Regiane G. Lima |
| collection | DOAJ |
| description | Antimicrobial photodynamic inactivation (aPDI) offers a promising alternative to combat drug-resistant bacteria. This study explores the potential of lapachol, a natural naphthoquinone derived from <i>Tabebuia avellanedae</i>, as a photosensitizer (PS) for aPDI. Lapachol’s photosensitizing properties were evaluated using <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> strains under blue LED light (450 nm). UV-vis spectroscopy confirmed lapachol’s absorption peak at 482 nm, aligning with effective excitation wavelengths for phototherapy. Photoinactivation assays demonstrated significant bacterial growth inhibition, achieving complete eradication of <i>S. aureus</i> at 25 µg·mL<sup>−1</sup> under light exposure. Scanning electron microscopy (SEM) revealed morphological damage in irradiated bacterial cells, confirming lapachol’s bactericidal effect. This research underscores lapachol’s potential as a novel photosensitizer in antimicrobial photodynamic therapy, addressing a critical need in combating antibiotic resistance. |
| format | Article |
| id | doaj-art-51c8804a2e71461e8b38a8c96cc5fee2 |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-51c8804a2e71461e8b38a8c96cc5fee22024-11-08T14:38:36ZengMDPI AGMolecules1420-30492024-11-012921518410.3390/molecules29215184Determination of Photosensitizing Potential of Lapachol for Photodynamic Inactivation of BacteriaRegiane G. Lima0Raphael S. Flores1Gabriella Miessi2Jhoenne H. V. Pulcherio3Laís F. Aguilera4Leandro O. Araujo5Samuel L. Oliveira6Anderson R. L. Caires7Optics and Photonics Group, Institute of Physics, Federal University of Mato Grosso do Sul, P.O. Box 549, 79070-900 Campo Grande, MS, BrazilOptics and Photonics Group, Institute of Physics, Federal University of Mato Grosso do Sul, P.O. Box 549, 79070-900 Campo Grande, MS, BrazilOptics and Photonics Group, Institute of Physics, Federal University of Mato Grosso do Sul, P.O. Box 549, 79070-900 Campo Grande, MS, BrazilOptics and Photonics Group, Institute of Physics, Federal University of Mato Grosso do Sul, P.O. Box 549, 79070-900 Campo Grande, MS, BrazilOptics and Photonics Group, Institute of Physics, Federal University of Mato Grosso do Sul, P.O. Box 549, 79070-900 Campo Grande, MS, BrazilOptics and Photonics Group, Institute of Physics, Federal University of Mato Grosso do Sul, P.O. Box 549, 79070-900 Campo Grande, MS, BrazilOptics and Photonics Group, Institute of Physics, Federal University of Mato Grosso do Sul, P.O. Box 549, 79070-900 Campo Grande, MS, BrazilOptics and Photonics Group, Institute of Physics, Federal University of Mato Grosso do Sul, P.O. Box 549, 79070-900 Campo Grande, MS, BrazilAntimicrobial photodynamic inactivation (aPDI) offers a promising alternative to combat drug-resistant bacteria. This study explores the potential of lapachol, a natural naphthoquinone derived from <i>Tabebuia avellanedae</i>, as a photosensitizer (PS) for aPDI. Lapachol’s photosensitizing properties were evaluated using <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> strains under blue LED light (450 nm). UV-vis spectroscopy confirmed lapachol’s absorption peak at 482 nm, aligning with effective excitation wavelengths for phototherapy. Photoinactivation assays demonstrated significant bacterial growth inhibition, achieving complete eradication of <i>S. aureus</i> at 25 µg·mL<sup>−1</sup> under light exposure. Scanning electron microscopy (SEM) revealed morphological damage in irradiated bacterial cells, confirming lapachol’s bactericidal effect. This research underscores lapachol’s potential as a novel photosensitizer in antimicrobial photodynamic therapy, addressing a critical need in combating antibiotic resistance.https://www.mdpi.com/1420-3049/29/21/5184lapacholblue lightantimicrobial photodynamic inactivationbacteria |
| spellingShingle | Regiane G. Lima Raphael S. Flores Gabriella Miessi Jhoenne H. V. Pulcherio Laís F. Aguilera Leandro O. Araujo Samuel L. Oliveira Anderson R. L. Caires Determination of Photosensitizing Potential of Lapachol for Photodynamic Inactivation of Bacteria Molecules lapachol blue light antimicrobial photodynamic inactivation bacteria |
| title | Determination of Photosensitizing Potential of Lapachol for Photodynamic Inactivation of Bacteria |
| title_full | Determination of Photosensitizing Potential of Lapachol for Photodynamic Inactivation of Bacteria |
| title_fullStr | Determination of Photosensitizing Potential of Lapachol for Photodynamic Inactivation of Bacteria |
| title_full_unstemmed | Determination of Photosensitizing Potential of Lapachol for Photodynamic Inactivation of Bacteria |
| title_short | Determination of Photosensitizing Potential of Lapachol for Photodynamic Inactivation of Bacteria |
| title_sort | determination of photosensitizing potential of lapachol for photodynamic inactivation of bacteria |
| topic | lapachol blue light antimicrobial photodynamic inactivation bacteria |
| url | https://www.mdpi.com/1420-3049/29/21/5184 |
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