miR-628-3p exerts a carcinogenic effect on hepatocellular carcinoma
Hepatocellular carcinoma (HCC) is associated with the highest mortality rate among various types of liver tumors. miR-628-3p, a microRNA, has been identified as a tumor suppressor in multiple cancer types, yet its function in hepatocellular carcinoma has not been investigated. This study aimed to ex...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
|
| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580825002730 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849229303811670016 |
|---|---|
| author | Lele Liu Ziyi Cheng Dina Liu Mingang Pan Muyu Luo Yunmeng Chen Jie Xia |
| author_facet | Lele Liu Ziyi Cheng Dina Liu Mingang Pan Muyu Luo Yunmeng Chen Jie Xia |
| author_sort | Lele Liu |
| collection | DOAJ |
| description | Hepatocellular carcinoma (HCC) is associated with the highest mortality rate among various types of liver tumors. miR-628-3p, a microRNA, has been identified as a tumor suppressor in multiple cancer types, yet its function in hepatocellular carcinoma has not been investigated. This study aimed to examine the effect of miR-628-3p on the occurrence and development of HCC and its specific molecular mechanism. Here, we evaluated the effect of miR-628-3p on HCC proliferation by using in vitro proliferation assays and a xenograft tumor model. Flow cytometry was used to monitor the cell cycle and apoptosis of HCC cells. Reverse Transcription-Quantitative Polymerase Chain Reaction (RT-qPCR) and Western blot (WB) were used to determine the expression of each gene. Our study showed that miR-628-3p levels decrease in HCC, and its overexpression can enhance cell proliferation and cell cycle progression while suppressing apoptosis. Examination of the gene expression profiles of MHCC9H cells with miR-628-3p overexpression shows that cancer-promoting pathways like hypoxia and Notch signaling are upregulated. Meanwhile, miR-628-3p overexpression inhibits tumor suppressor pathways such as apoptosis and p53 signaling. miR-628-3p affects the cell cycle and apoptosis of HCC through the p53 pathway. Moreover, the expression of miR-628-3p is regulated by p53 to some extent. Our findings suggest that miR-628-3p has a tumor-promoting effect on HCC and that miR-628-3p inhibitors may be a new therapeutic approach for HCC. |
| format | Article |
| id | doaj-art-51a5df2ec6c14a56a19abed7107d57c7 |
| institution | Kabale University |
| issn | 2405-5808 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj-art-51a5df2ec6c14a56a19abed7107d57c72025-08-22T04:56:54ZengElsevierBiochemistry and Biophysics Reports2405-58082025-09-014310218610.1016/j.bbrep.2025.102186miR-628-3p exerts a carcinogenic effect on hepatocellular carcinomaLele Liu0Ziyi Cheng1Dina Liu2Mingang Pan3Muyu Luo4Yunmeng Chen5Jie Xia6Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Chongqing Medical University, Chongqing, 400016, PR ChinaKey Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Chongqing Medical University, Chongqing, 400016, PR ChinaInstitute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, PR ChinaKey Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Chongqing Medical University, Chongqing, 400016, PR ChinaKey Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Chongqing Medical University, Chongqing, 400016, PR ChinaKey Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Chongqing Medical University, Chongqing, 400016, PR ChinaKey Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Chongqing Medical University, Chongqing, 400016, PR China; Western (Chongqing) Institute for Digital-Intelligent Medicine, Chongqing, 401329, PR China; Corresponding author. 1 Yixue Yuan Road, Chongqing, 400016, PR China.Hepatocellular carcinoma (HCC) is associated with the highest mortality rate among various types of liver tumors. miR-628-3p, a microRNA, has been identified as a tumor suppressor in multiple cancer types, yet its function in hepatocellular carcinoma has not been investigated. This study aimed to examine the effect of miR-628-3p on the occurrence and development of HCC and its specific molecular mechanism. Here, we evaluated the effect of miR-628-3p on HCC proliferation by using in vitro proliferation assays and a xenograft tumor model. Flow cytometry was used to monitor the cell cycle and apoptosis of HCC cells. Reverse Transcription-Quantitative Polymerase Chain Reaction (RT-qPCR) and Western blot (WB) were used to determine the expression of each gene. Our study showed that miR-628-3p levels decrease in HCC, and its overexpression can enhance cell proliferation and cell cycle progression while suppressing apoptosis. Examination of the gene expression profiles of MHCC9H cells with miR-628-3p overexpression shows that cancer-promoting pathways like hypoxia and Notch signaling are upregulated. Meanwhile, miR-628-3p overexpression inhibits tumor suppressor pathways such as apoptosis and p53 signaling. miR-628-3p affects the cell cycle and apoptosis of HCC through the p53 pathway. Moreover, the expression of miR-628-3p is regulated by p53 to some extent. Our findings suggest that miR-628-3p has a tumor-promoting effect on HCC and that miR-628-3p inhibitors may be a new therapeutic approach for HCC.http://www.sciencedirect.com/science/article/pii/S2405580825002730ApoptosisCell cycleHCCmiR-628-3pProliferationp53 |
| spellingShingle | Lele Liu Ziyi Cheng Dina Liu Mingang Pan Muyu Luo Yunmeng Chen Jie Xia miR-628-3p exerts a carcinogenic effect on hepatocellular carcinoma Biochemistry and Biophysics Reports Apoptosis Cell cycle HCC miR-628-3p Proliferation p53 |
| title | miR-628-3p exerts a carcinogenic effect on hepatocellular carcinoma |
| title_full | miR-628-3p exerts a carcinogenic effect on hepatocellular carcinoma |
| title_fullStr | miR-628-3p exerts a carcinogenic effect on hepatocellular carcinoma |
| title_full_unstemmed | miR-628-3p exerts a carcinogenic effect on hepatocellular carcinoma |
| title_short | miR-628-3p exerts a carcinogenic effect on hepatocellular carcinoma |
| title_sort | mir 628 3p exerts a carcinogenic effect on hepatocellular carcinoma |
| topic | Apoptosis Cell cycle HCC miR-628-3p Proliferation p53 |
| url | http://www.sciencedirect.com/science/article/pii/S2405580825002730 |
| work_keys_str_mv | AT leleliu mir6283pexertsacarcinogeniceffectonhepatocellularcarcinoma AT ziyicheng mir6283pexertsacarcinogeniceffectonhepatocellularcarcinoma AT dinaliu mir6283pexertsacarcinogeniceffectonhepatocellularcarcinoma AT mingangpan mir6283pexertsacarcinogeniceffectonhepatocellularcarcinoma AT muyuluo mir6283pexertsacarcinogeniceffectonhepatocellularcarcinoma AT yunmengchen mir6283pexertsacarcinogeniceffectonhepatocellularcarcinoma AT jiexia mir6283pexertsacarcinogeniceffectonhepatocellularcarcinoma |