Exploring the Influence of Biochar-Supported Nano-Iron Oxide on Phosphorus Speciation Transformation and Bacterial Community Structure in Aerobic Pig Manure Composting Processes

Existing studies have demonstrated the positive effects of nano-sized iron oxide on compost maturity, yet the impact of nano-sized iron oxide on phosphorus speciation and bacterial communities during the composting process remains unclear. In this study, pig manure and straw were used as raw materia...

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Main Authors: Ning Yuan, Kang Wang, Mengyue Liang, Jia Zhou, Rui Yu
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/12/12/2593
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author Ning Yuan
Kang Wang
Mengyue Liang
Jia Zhou
Rui Yu
author_facet Ning Yuan
Kang Wang
Mengyue Liang
Jia Zhou
Rui Yu
author_sort Ning Yuan
collection DOAJ
description Existing studies have demonstrated the positive effects of nano-sized iron oxide on compost maturity, yet the impact of nano-sized iron oxide on phosphorus speciation and bacterial communities during the composting process remains unclear. In this study, pig manure and straw were used as raw materials, with biochar-supported nano-sized iron oxide (BC-Fe<sub>3</sub>O<sub>4</sub>NPs) as an additive and calcium peroxide (CaO<sub>2</sub>) as a co-agent, to conduct an aerobic composting experiment with pig manure. Four treatments were tested: CK (control), F1 (1% BC-Fe<sub>3</sub>O<sub>4</sub>NPs), F2 (5% BC-Fe<sub>3</sub>O<sub>4</sub>NPs), and F3 (5% BC-Fe<sub>3</sub>O<sub>4</sub>NPs + 5% CaO<sub>2</sub>). Key findings include the following. (1) BC-Fe<sub>3</sub>O<sub>4</sub>NPs increased compost temperatures, with F3 reaching 61℃; F1 showed optimal maturity (C/N ratio: 12.90). (2) BC-Fe<sub>3</sub>O<sub>4</sub>NPs promoted stable phosphorus forms; Residual-P proportions were higher in F1, F2, and F3 (25.81%, 51.16%, 51.68%) than CK (19.32%). (3) Bacterial phyla <i>Firmicutes</i>, <i>Actinobacteria,</i> and <i>Proteobacteria</i> dominated. BC-Fe<sub>3</sub>O<sub>4</sub>NPs altered community composition, especially on day 7. <i>Firmicutes</i> dominated CK, F1, and F3; <i>Proteobacteria</i> dominated F2. At the genus level, day 7 showed <i>Corynebacterium</i> (CK), <i>Clostridum</i> (F1, F3), and <i>Caldibacillus</i> (F2) as predominant. (4) Pearson correlation analysis revealed shifted correlations between phosphorus forms and bacterial phyla after BC-Fe<sub>3</sub>O<sub>4</sub>NPs addition. <i>Firmicutes</i> positively correlated with NaOH-OP in F1 during the thermophilic phase, facilitating phosphate release and adsorption by BC-Fe<sub>3</sub>O<sub>4</sub>NPs. The significance of correlations diminished with increasing additive concentration; in F3, all phyla positively correlated with various phosphorus forms.
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spelling doaj-art-51a31587bb7a4ba6aaa0fb4c7ed41f7f2024-12-27T14:41:40ZengMDPI AGMicroorganisms2076-26072024-12-011212259310.3390/microorganisms12122593Exploring the Influence of Biochar-Supported Nano-Iron Oxide on Phosphorus Speciation Transformation and Bacterial Community Structure in Aerobic Pig Manure Composting ProcessesNing Yuan0Kang Wang1Mengyue Liang2Jia Zhou3Rui Yu4College of Geographic Science, Harbin Normal University, Harbin 150025, ChinaCollege of Geographic Science, Harbin Normal University, Harbin 150025, ChinaCollege of Geographic Science, Harbin Normal University, Harbin 150025, ChinaCollege of Geographic Science, Harbin Normal University, Harbin 150025, ChinaState Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, ChinaExisting studies have demonstrated the positive effects of nano-sized iron oxide on compost maturity, yet the impact of nano-sized iron oxide on phosphorus speciation and bacterial communities during the composting process remains unclear. In this study, pig manure and straw were used as raw materials, with biochar-supported nano-sized iron oxide (BC-Fe<sub>3</sub>O<sub>4</sub>NPs) as an additive and calcium peroxide (CaO<sub>2</sub>) as a co-agent, to conduct an aerobic composting experiment with pig manure. Four treatments were tested: CK (control), F1 (1% BC-Fe<sub>3</sub>O<sub>4</sub>NPs), F2 (5% BC-Fe<sub>3</sub>O<sub>4</sub>NPs), and F3 (5% BC-Fe<sub>3</sub>O<sub>4</sub>NPs + 5% CaO<sub>2</sub>). Key findings include the following. (1) BC-Fe<sub>3</sub>O<sub>4</sub>NPs increased compost temperatures, with F3 reaching 61℃; F1 showed optimal maturity (C/N ratio: 12.90). (2) BC-Fe<sub>3</sub>O<sub>4</sub>NPs promoted stable phosphorus forms; Residual-P proportions were higher in F1, F2, and F3 (25.81%, 51.16%, 51.68%) than CK (19.32%). (3) Bacterial phyla <i>Firmicutes</i>, <i>Actinobacteria,</i> and <i>Proteobacteria</i> dominated. BC-Fe<sub>3</sub>O<sub>4</sub>NPs altered community composition, especially on day 7. <i>Firmicutes</i> dominated CK, F1, and F3; <i>Proteobacteria</i> dominated F2. At the genus level, day 7 showed <i>Corynebacterium</i> (CK), <i>Clostridum</i> (F1, F3), and <i>Caldibacillus</i> (F2) as predominant. (4) Pearson correlation analysis revealed shifted correlations between phosphorus forms and bacterial phyla after BC-Fe<sub>3</sub>O<sub>4</sub>NPs addition. <i>Firmicutes</i> positively correlated with NaOH-OP in F1 during the thermophilic phase, facilitating phosphate release and adsorption by BC-Fe<sub>3</sub>O<sub>4</sub>NPs. The significance of correlations diminished with increasing additive concentration; in F3, all phyla positively correlated with various phosphorus forms.https://www.mdpi.com/2076-2607/12/12/2593BC-Fe<sub>3</sub>O<sub>4</sub>NPsaerobic compostingphosphorus speciationmetagenomics
spellingShingle Ning Yuan
Kang Wang
Mengyue Liang
Jia Zhou
Rui Yu
Exploring the Influence of Biochar-Supported Nano-Iron Oxide on Phosphorus Speciation Transformation and Bacterial Community Structure in Aerobic Pig Manure Composting Processes
Microorganisms
BC-Fe<sub>3</sub>O<sub>4</sub>NPs
aerobic composting
phosphorus speciation
metagenomics
title Exploring the Influence of Biochar-Supported Nano-Iron Oxide on Phosphorus Speciation Transformation and Bacterial Community Structure in Aerobic Pig Manure Composting Processes
title_full Exploring the Influence of Biochar-Supported Nano-Iron Oxide on Phosphorus Speciation Transformation and Bacterial Community Structure in Aerobic Pig Manure Composting Processes
title_fullStr Exploring the Influence of Biochar-Supported Nano-Iron Oxide on Phosphorus Speciation Transformation and Bacterial Community Structure in Aerobic Pig Manure Composting Processes
title_full_unstemmed Exploring the Influence of Biochar-Supported Nano-Iron Oxide on Phosphorus Speciation Transformation and Bacterial Community Structure in Aerobic Pig Manure Composting Processes
title_short Exploring the Influence of Biochar-Supported Nano-Iron Oxide on Phosphorus Speciation Transformation and Bacterial Community Structure in Aerobic Pig Manure Composting Processes
title_sort exploring the influence of biochar supported nano iron oxide on phosphorus speciation transformation and bacterial community structure in aerobic pig manure composting processes
topic BC-Fe<sub>3</sub>O<sub>4</sub>NPs
aerobic composting
phosphorus speciation
metagenomics
url https://www.mdpi.com/2076-2607/12/12/2593
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