stilPCR increases the effective sequencing length of Illumina targeted next-generation sequencing.
Identifying pathogens, resistance-conferring mutations, and strain types through targeted amplicon sequencing is an important tool. However, due to the limitations of short read sequencing, many applications require the division of limited clinical samples. Here, we present stilPCR (single-tube Illu...
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Main Authors: | Jason D Limberis, Roland J Nagel, Soumitesh Chakravorty, Dena Block, Scott Dewell, Alina Nalyvayko, Zach Howard, Grant Theron, Rouxjeane Venter, John Z Metcalfe |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2024-01-01
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Series: | PLoS ONE |
Online Access: | https://doi.org/10.1371/journal.pone.0314265 |
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