Detection of antibodies against H5 subtype highly pathogenic avian influenza viruses in multiple raccoons in Tokachi District, Hokkaido, Japan, from 2022 to 2023
In recent years, infection cases of H5 subtype highly pathogenic avian influenza viruses (HPAIVs) in wild mammals have increased globally. To obtain recent epidemiological information regarding influenza A virus (IAV) infection in raccoons (Procyon lotor), the prevalence of anti-IAV antibodies in se...
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Elsevier
2025-01-01
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| Series: | Virus Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0168170224002089 |
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| author | Minami Komami James G. Komu Yuki Ishiguro Motoki Sasaki Sachiko Matsuda Dulamjav Jamsransuren Vuong Nghia Bui Yohei Watanabe Kunitoshi Imai Haruko Ogawa Yohei Takeda |
| author_facet | Minami Komami James G. Komu Yuki Ishiguro Motoki Sasaki Sachiko Matsuda Dulamjav Jamsransuren Vuong Nghia Bui Yohei Watanabe Kunitoshi Imai Haruko Ogawa Yohei Takeda |
| author_sort | Minami Komami |
| collection | DOAJ |
| description | In recent years, infection cases of H5 subtype highly pathogenic avian influenza viruses (HPAIVs) in wild mammals have increased globally. To obtain recent epidemiological information regarding influenza A virus (IAV) infection in raccoons (Procyon lotor), the prevalence of anti-IAV antibodies in sera was analyzed among raccoons captured in Tokachi District, Hokkaido, Japan, from 2019 to 2023. Screening of serum samples using enzyme-linked immunosorbent assay and agar gel precipitation test detected anti-IAV antibodies in 5 of 114 (4.4 %) raccoons. All positive sera were from raccoons captured from 2022 to 2023. The hemagglutination inhibition test revealed that all five serum samples contained anti-H5 subtype HPAIV antibodies, and one also contained anti-H1 subtype antibodies. The neuraminidase inhibition test revealed that all five sera contained anti-N1 subtype antibodies, and one also contained anti-N8 subtype antibodies. In the virus neutralization test, these five sera showed stronger neutralization activity against the H5 subtype clade 2.3.4.4b HPAIV strain recently circulating worldwide compared to the old H5 HPAIV strain isolated in Japan in 2007. These findings suggested that raccoons could be involved in the circulation of H5 HPAIVs in nature. |
| format | Article |
| id | doaj-art-50eecfb60ced434c8f612282ca614cea |
| institution | Kabale University |
| issn | 1872-7492 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Virus Research |
| spelling | doaj-art-50eecfb60ced434c8f612282ca614cea2025-01-09T06:12:15ZengElsevierVirus Research1872-74922025-01-01351199515Detection of antibodies against H5 subtype highly pathogenic avian influenza viruses in multiple raccoons in Tokachi District, Hokkaido, Japan, from 2022 to 2023Minami Komami0James G. Komu1Yuki Ishiguro2Motoki Sasaki3Sachiko Matsuda4Dulamjav Jamsransuren5Vuong Nghia Bui6Yohei Watanabe7Kunitoshi Imai8Haruko Ogawa9Yohei Takeda10Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, JapanGraduate School of Animal and Veterinary Sciences and Agriculture, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, Japan; Department of Medical Laboratory Sciences, College of Health Sciences, Jomo Kenyatta University of Agriculture and Technology, Nairobi P.O. Box 62000-00200, KenyaDepartment of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, JapanDepartment of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, JapanResearch Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, JapanResearch Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, JapanVirology Department, National Institute of Veterinary Research, Hanoi 100000, VietnamDepartment of Infectious Diseases, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan; Department of Virology, The JIKEI University School of Medicine, Tokyo 105-8461, JapanDepartment of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, JapanDepartment of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, JapanDepartment of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, Japan; Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, Japan; Correspondence author at: Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, 2-11 Inada, Obihiro, Hokkaido 080-8555, Japan.In recent years, infection cases of H5 subtype highly pathogenic avian influenza viruses (HPAIVs) in wild mammals have increased globally. To obtain recent epidemiological information regarding influenza A virus (IAV) infection in raccoons (Procyon lotor), the prevalence of anti-IAV antibodies in sera was analyzed among raccoons captured in Tokachi District, Hokkaido, Japan, from 2019 to 2023. Screening of serum samples using enzyme-linked immunosorbent assay and agar gel precipitation test detected anti-IAV antibodies in 5 of 114 (4.4 %) raccoons. All positive sera were from raccoons captured from 2022 to 2023. The hemagglutination inhibition test revealed that all five serum samples contained anti-H5 subtype HPAIV antibodies, and one also contained anti-H1 subtype antibodies. The neuraminidase inhibition test revealed that all five sera contained anti-N1 subtype antibodies, and one also contained anti-N8 subtype antibodies. In the virus neutralization test, these five sera showed stronger neutralization activity against the H5 subtype clade 2.3.4.4b HPAIV strain recently circulating worldwide compared to the old H5 HPAIV strain isolated in Japan in 2007. These findings suggested that raccoons could be involved in the circulation of H5 HPAIVs in nature.http://www.sciencedirect.com/science/article/pii/S0168170224002089H5 subtype highly pathogenic avian influenza virusClade 2.3.4.4bRaccoonSeroprevalence |
| spellingShingle | Minami Komami James G. Komu Yuki Ishiguro Motoki Sasaki Sachiko Matsuda Dulamjav Jamsransuren Vuong Nghia Bui Yohei Watanabe Kunitoshi Imai Haruko Ogawa Yohei Takeda Detection of antibodies against H5 subtype highly pathogenic avian influenza viruses in multiple raccoons in Tokachi District, Hokkaido, Japan, from 2022 to 2023 Virus Research H5 subtype highly pathogenic avian influenza virus Clade 2.3.4.4b Raccoon Seroprevalence |
| title | Detection of antibodies against H5 subtype highly pathogenic avian influenza viruses in multiple raccoons in Tokachi District, Hokkaido, Japan, from 2022 to 2023 |
| title_full | Detection of antibodies against H5 subtype highly pathogenic avian influenza viruses in multiple raccoons in Tokachi District, Hokkaido, Japan, from 2022 to 2023 |
| title_fullStr | Detection of antibodies against H5 subtype highly pathogenic avian influenza viruses in multiple raccoons in Tokachi District, Hokkaido, Japan, from 2022 to 2023 |
| title_full_unstemmed | Detection of antibodies against H5 subtype highly pathogenic avian influenza viruses in multiple raccoons in Tokachi District, Hokkaido, Japan, from 2022 to 2023 |
| title_short | Detection of antibodies against H5 subtype highly pathogenic avian influenza viruses in multiple raccoons in Tokachi District, Hokkaido, Japan, from 2022 to 2023 |
| title_sort | detection of antibodies against h5 subtype highly pathogenic avian influenza viruses in multiple raccoons in tokachi district hokkaido japan from 2022 to 2023 |
| topic | H5 subtype highly pathogenic avian influenza virus Clade 2.3.4.4b Raccoon Seroprevalence |
| url | http://www.sciencedirect.com/science/article/pii/S0168170224002089 |
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