Mutational Analysis of BRAFV600E by Polymerase Chain Reaction in Breast Cancer: A Narrative Review
A major cause of cancer-related death for women globally is breast cancer, calling for molecular insights to better prognosis and treatment. One of the genetic changes that contributes to the constitutive activation of the MAPK/ERK (mitogen-activated protein kinase/extracellular signal-regulated kin...
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JCDR Research and Publications Private Limited
2025-01-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://www.jcdr.net/articles/PDF/20466/74252_CE[Ra1]_F(SHU)_QC(SD_SS)_PF1(AG_SS)_PFA(NC)_PN(IS).pdf |
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| author | Simran Khan Arvind Bhake |
| author_facet | Simran Khan Arvind Bhake |
| author_sort | Simran Khan |
| collection | DOAJ |
| description | A major cause of cancer-related death for women globally is breast cancer, calling for molecular insights to better prognosis and treatment. One of the genetic changes that contributes to the constitutive activation of the MAPK/ERK (mitogen-activated protein kinase/extracellular signal-regulated kinase) pathway and promoting carcinogenesis is the BRAFV600E mutation, which has been extensively studied in melanoma. BRAFV600E has been associated with aggressive forms of breast cancer, including Triple-Negative Breast Cancer (TNBC), and worse clinical outcomes, despite its rarity. The Polymerase Chain Reaction (PCR)-based methods for analysing BRAFV600E mutations in breast cancer, specifically Invasive Ductal Carcinoma (IDC), are the main topic of this narrative review. The sensitivity and precision of different PCR techniques, such as droplet digital PCR (ddPCR), qPCR and traditional PCR, in identifying this mutation vary. The review emphasises BRAFV600E potential as a therapeutic target and prognostic marker, particularly considering the poorer efficacy of BRAF inhibitors in treating breast cancer than melanoma. The available data, which mostly comes from non Indian populations, highlights the need for additional thorough studies in a variety of populations, including Indian women, to fully comprehend the clinical significance of the mutation. |
| format | Article |
| id | doaj-art-50cc5f971d5149529fa4f1b13e596d64 |
| institution | Kabale University |
| issn | 2249-782X 0973-709X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj-art-50cc5f971d5149529fa4f1b13e596d642025-01-11T12:15:31ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2025-01-011901010410.7860/JCDR/2025/74252.20466Mutational Analysis of BRAFV600E by Polymerase Chain Reaction in Breast Cancer: A Narrative ReviewSimran Khan0Arvind Bhake1Junior Resident, Department of Pathology, Datta Meghe Institute of Medical Sciences, Sawangi (Meghe), Maharashtra, IndiaDirector Professor, Department of Pathology, Datta Meghe Institute of Medical Sciences, Sawangi (Meghe), Maharashtra, India.A major cause of cancer-related death for women globally is breast cancer, calling for molecular insights to better prognosis and treatment. One of the genetic changes that contributes to the constitutive activation of the MAPK/ERK (mitogen-activated protein kinase/extracellular signal-regulated kinase) pathway and promoting carcinogenesis is the BRAFV600E mutation, which has been extensively studied in melanoma. BRAFV600E has been associated with aggressive forms of breast cancer, including Triple-Negative Breast Cancer (TNBC), and worse clinical outcomes, despite its rarity. The Polymerase Chain Reaction (PCR)-based methods for analysing BRAFV600E mutations in breast cancer, specifically Invasive Ductal Carcinoma (IDC), are the main topic of this narrative review. The sensitivity and precision of different PCR techniques, such as droplet digital PCR (ddPCR), qPCR and traditional PCR, in identifying this mutation vary. The review emphasises BRAFV600E potential as a therapeutic target and prognostic marker, particularly considering the poorer efficacy of BRAF inhibitors in treating breast cancer than melanoma. The available data, which mostly comes from non Indian populations, highlights the need for additional thorough studies in a variety of populations, including Indian women, to fully comprehend the clinical significance of the mutation.https://www.jcdr.net/articles/PDF/20466/74252_CE[Ra1]_F(SHU)_QC(SD_SS)_PF1(AG_SS)_PFA(NC)_PN(IS).pdfinvasive ductal carcinomaoncogenetriple-negative tumour biomarkers |
| spellingShingle | Simran Khan Arvind Bhake Mutational Analysis of BRAFV600E by Polymerase Chain Reaction in Breast Cancer: A Narrative Review Journal of Clinical and Diagnostic Research invasive ductal carcinoma oncogene triple-negative tumour biomarkers |
| title | Mutational Analysis of BRAFV600E by Polymerase Chain Reaction in Breast Cancer: A Narrative Review |
| title_full | Mutational Analysis of BRAFV600E by Polymerase Chain Reaction in Breast Cancer: A Narrative Review |
| title_fullStr | Mutational Analysis of BRAFV600E by Polymerase Chain Reaction in Breast Cancer: A Narrative Review |
| title_full_unstemmed | Mutational Analysis of BRAFV600E by Polymerase Chain Reaction in Breast Cancer: A Narrative Review |
| title_short | Mutational Analysis of BRAFV600E by Polymerase Chain Reaction in Breast Cancer: A Narrative Review |
| title_sort | mutational analysis of brafv600e by polymerase chain reaction in breast cancer a narrative review |
| topic | invasive ductal carcinoma oncogene triple-negative tumour biomarkers |
| url | https://www.jcdr.net/articles/PDF/20466/74252_CE[Ra1]_F(SHU)_QC(SD_SS)_PF1(AG_SS)_PFA(NC)_PN(IS).pdf |
| work_keys_str_mv | AT simrankhan mutationalanalysisofbrafv600ebypolymerasechainreactioninbreastcanceranarrativereview AT arvindbhake mutationalanalysisofbrafv600ebypolymerasechainreactioninbreastcanceranarrativereview |