The effect of constitutive root isoprene emission on root phenotype and physiology under control and salt stress conditions
Abstract Isoprene, a volatile hydrocarbon, is typically emitted from the leaves of many plant species. Given its well‐known function in plant growth and defense aboveground, we examined its effects on root physiology. We used isoprene‐emitting (IE) lines and a non‐emitting (NE) line of Arabidopsis a...
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| Format: | Article |
| Language: | English |
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Wiley
2024-07-01
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| Series: | Plant Direct |
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| Online Access: | https://doi.org/10.1002/pld3.617 |
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| author | Manuel Bellucci Mohammad Golam Mostofa Sarathi M. Weraduwage Yuan Xu Mostafa Abdelrahman Laura De Gara Francesco Loreto Thomas D. Sharkey |
| author_facet | Manuel Bellucci Mohammad Golam Mostofa Sarathi M. Weraduwage Yuan Xu Mostafa Abdelrahman Laura De Gara Francesco Loreto Thomas D. Sharkey |
| author_sort | Manuel Bellucci |
| collection | DOAJ |
| description | Abstract Isoprene, a volatile hydrocarbon, is typically emitted from the leaves of many plant species. Given its well‐known function in plant growth and defense aboveground, we examined its effects on root physiology. We used isoprene‐emitting (IE) lines and a non‐emitting (NE) line of Arabidopsis and investigated their performance by analyzing root phenotype, hormone levels, transcriptome, and metabolite profiles under both normal and salt stress conditions. We show that IE lines emitted tiny amounts of isoprene from roots and showed an increased root/shoot ratio compared with NE line. Isoprene emission exerted a noteworthy influence on hormone profiles related to plant growth and stress response, promoting root development and salt‐stress resistance. Methyl erythritol 4‐phosphate pathway metabolites, precursors of isoprene and hormones, were higher in the roots of IE lines than in the NE line. Transcriptome data indicated that the presence of isoprene increased the expression of key genes involved in hormone metabolism/signaling. Our findings reveal that constitutive root isoprene emission sustains root growth under saline conditions by regulating and/or priming hormone biosynthesis and signaling mechanisms and expression of key genes relevant to salt stress defense. |
| format | Article |
| id | doaj-art-50c62b782ebb48fc8ff46ad0f12d764a |
| institution | Kabale University |
| issn | 2475-4455 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj-art-50c62b782ebb48fc8ff46ad0f12d764a2025-01-06T07:21:54ZengWileyPlant Direct2475-44552024-07-0187n/an/a10.1002/pld3.617The effect of constitutive root isoprene emission on root phenotype and physiology under control and salt stress conditionsManuel Bellucci0Mohammad Golam Mostofa1Sarathi M. Weraduwage2Yuan Xu3Mostafa Abdelrahman4Laura De Gara5Francesco Loreto6Thomas D. Sharkey7Department of Energy Plant Research Laboratory Michigan State University East Lansing Michigan USADepartment of Energy Plant Research Laboratory Michigan State University East Lansing Michigan USADepartment of Biology and Biochemistry Bishop's University Sherbrooke Quebec CanadaDepartment of Energy Plant Research Laboratory Michigan State University East Lansing Michigan USAInstitute of Genomics for Crop Abiotic Stress Tolerance Texas Tech University Lubbock Texas USADepartment of Science and Technology for Humans and the Environment Università Campus Bio‐Medico di Roma Rome ItalyDepartment of Biology University of Naples Federico II Naples ItalyDepartment of Energy Plant Research Laboratory Michigan State University East Lansing Michigan USAAbstract Isoprene, a volatile hydrocarbon, is typically emitted from the leaves of many plant species. Given its well‐known function in plant growth and defense aboveground, we examined its effects on root physiology. We used isoprene‐emitting (IE) lines and a non‐emitting (NE) line of Arabidopsis and investigated their performance by analyzing root phenotype, hormone levels, transcriptome, and metabolite profiles under both normal and salt stress conditions. We show that IE lines emitted tiny amounts of isoprene from roots and showed an increased root/shoot ratio compared with NE line. Isoprene emission exerted a noteworthy influence on hormone profiles related to plant growth and stress response, promoting root development and salt‐stress resistance. Methyl erythritol 4‐phosphate pathway metabolites, precursors of isoprene and hormones, were higher in the roots of IE lines than in the NE line. Transcriptome data indicated that the presence of isoprene increased the expression of key genes involved in hormone metabolism/signaling. Our findings reveal that constitutive root isoprene emission sustains root growth under saline conditions by regulating and/or priming hormone biosynthesis and signaling mechanisms and expression of key genes relevant to salt stress defense.https://doi.org/10.1002/pld3.617cytokininsisoprene synthasemethylerythritol 4‐phosphate (MEP) metabolitesroot phenotypesalinitytranscriptomics |
| spellingShingle | Manuel Bellucci Mohammad Golam Mostofa Sarathi M. Weraduwage Yuan Xu Mostafa Abdelrahman Laura De Gara Francesco Loreto Thomas D. Sharkey The effect of constitutive root isoprene emission on root phenotype and physiology under control and salt stress conditions Plant Direct cytokinins isoprene synthase methylerythritol 4‐phosphate (MEP) metabolites root phenotype salinity transcriptomics |
| title | The effect of constitutive root isoprene emission on root phenotype and physiology under control and salt stress conditions |
| title_full | The effect of constitutive root isoprene emission on root phenotype and physiology under control and salt stress conditions |
| title_fullStr | The effect of constitutive root isoprene emission on root phenotype and physiology under control and salt stress conditions |
| title_full_unstemmed | The effect of constitutive root isoprene emission on root phenotype and physiology under control and salt stress conditions |
| title_short | The effect of constitutive root isoprene emission on root phenotype and physiology under control and salt stress conditions |
| title_sort | effect of constitutive root isoprene emission on root phenotype and physiology under control and salt stress conditions |
| topic | cytokinins isoprene synthase methylerythritol 4‐phosphate (MEP) metabolites root phenotype salinity transcriptomics |
| url | https://doi.org/10.1002/pld3.617 |
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