Effect of cooling and freezing on the kinematic parameters of ram spermatozoa sexed by modified protocol with TLR7/8 ligand R848
The present study investigated the effect of sex-sorting by TLR7/8 ligand R848, cooling and cryopreservation on the motility and kinematic characteristics of ram spermatozoa for the first time. Four ejaculates per ram (n=2) were collected, pooled and after that were split to 8 equal parts. Four part...
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Institute for Animal Husbandry, Belgrade
2024-01-01
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| Series: | Biotechnology in Animal Husbandry |
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| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1450-9156/2024/1450-91562402087Y.pdf |
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| author | Yotov Stanimir Kistanova Elena Atanasov Anatoli Ivanova Boyana Zaimova Darina |
| author_facet | Yotov Stanimir Kistanova Elena Atanasov Anatoli Ivanova Boyana Zaimova Darina |
| author_sort | Yotov Stanimir |
| collection | DOAJ |
| description | The present study investigated the effect of sex-sorting by TLR7/8 ligand R848, cooling and cryopreservation on the motility and kinematic characteristics of ram spermatozoa for the first time. Four ejaculates per ram (n=2) were collected, pooled and after that were split to 8 equal parts. Four parts were used for sexing and four parts were analyzed as unsexed semen. The sperm sexing was done by application of the TLR7/8 ligand R848 (resiquimod). Diluted with freezing extender unsexed and sex-sorted semen were cooled in a refrigerator at temperature 5°C for 5 hours and after that freeze/thawed. Each part of semen at each step of the experiment was subjected to computer assisted semen analysis for evaluation of motility and speed parameters of spermatozoa. After sexing the significant reducing of kinematic parameters in both fractions were observed. The dilution of sexed spermatozoa with freezing extender before cooling reliably increased the progressive motility and speed parameters as VCL, VAP, and VSL in Xand Y-fractions and kept them during the cooling at relatively high level. Freezing adversely impaired the motility of sexed spermatozoa. The values of speed parameters of unsexed spermatozoa after thawing were significantly higher than those of X and Y spermatozoa. Dilution with an appropriate freezing extender and cooling are most suitable approaches for storing of sexed ram spermatozoa. |
| format | Article |
| id | doaj-art-5087356865b34de4b617c6da050b56e3 |
| institution | Kabale University |
| issn | 1450-9156 2217-7140 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Institute for Animal Husbandry, Belgrade |
| record_format | Article |
| series | Biotechnology in Animal Husbandry |
| spelling | doaj-art-5087356865b34de4b617c6da050b56e32025-01-08T15:19:49ZengInstitute for Animal Husbandry, BelgradeBiotechnology in Animal Husbandry1450-91562217-71402024-01-01402879910.2298/BAH2402087Y1450-91562402087YEffect of cooling and freezing on the kinematic parameters of ram spermatozoa sexed by modified protocol with TLR7/8 ligand R848Yotov Stanimir0https://orcid.org/0000-0002-9435-6854Kistanova Elena1Atanasov Anatoli2https://orcid.org/0000-0002-0237-8555Ivanova Boyana3https://orcid.org/0000-0002-8934-9609Zaimova Darina4https://orcid.org/0000-0001-6971-3701Trakia University, Faculty of Veterinary Medicine, Reproduction and Reproductive Disorders, Department of Obstetrics, Student Campus, Stara Zagora, BulgariaTrakia University, Faculty of Veterinary Medicine, Reproduction and Reproductive Disorders, Department of Obstetrics, Student Campus, Stara Zagora, BulgariaTrakia University, Faculty of Veterinary Medicine, Reproduction and Reproductive Disorders, Department of Obstetrics, Student Campus, Stara Zagora, BulgariaTrakia University, Faculty of Veterinary Medicine, Reproduction and Reproductive Disorders, Department of Obstetrics, Student Campus, Stara Zagora, BulgariaTrakia University, Faculty of Economics, Department of Industrial Business and Entrepreneurship, Student Campus, Stara Zagora, BulgariaThe present study investigated the effect of sex-sorting by TLR7/8 ligand R848, cooling and cryopreservation on the motility and kinematic characteristics of ram spermatozoa for the first time. Four ejaculates per ram (n=2) were collected, pooled and after that were split to 8 equal parts. Four parts were used for sexing and four parts were analyzed as unsexed semen. The sperm sexing was done by application of the TLR7/8 ligand R848 (resiquimod). Diluted with freezing extender unsexed and sex-sorted semen were cooled in a refrigerator at temperature 5°C for 5 hours and after that freeze/thawed. Each part of semen at each step of the experiment was subjected to computer assisted semen analysis for evaluation of motility and speed parameters of spermatozoa. After sexing the significant reducing of kinematic parameters in both fractions were observed. The dilution of sexed spermatozoa with freezing extender before cooling reliably increased the progressive motility and speed parameters as VCL, VAP, and VSL in Xand Y-fractions and kept them during the cooling at relatively high level. Freezing adversely impaired the motility of sexed spermatozoa. The values of speed parameters of unsexed spermatozoa after thawing were significantly higher than those of X and Y spermatozoa. Dilution with an appropriate freezing extender and cooling are most suitable approaches for storing of sexed ram spermatozoa.https://scindeks-clanci.ceon.rs/data/pdf/1450-9156/2024/1450-91562402087Y.pdfram semen sexingtlr7/8 ligand r848kinematicscoolingfreezing |
| spellingShingle | Yotov Stanimir Kistanova Elena Atanasov Anatoli Ivanova Boyana Zaimova Darina Effect of cooling and freezing on the kinematic parameters of ram spermatozoa sexed by modified protocol with TLR7/8 ligand R848 Biotechnology in Animal Husbandry ram semen sexing tlr7/8 ligand r848 kinematics cooling freezing |
| title | Effect of cooling and freezing on the kinematic parameters of ram spermatozoa sexed by modified protocol with TLR7/8 ligand R848 |
| title_full | Effect of cooling and freezing on the kinematic parameters of ram spermatozoa sexed by modified protocol with TLR7/8 ligand R848 |
| title_fullStr | Effect of cooling and freezing on the kinematic parameters of ram spermatozoa sexed by modified protocol with TLR7/8 ligand R848 |
| title_full_unstemmed | Effect of cooling and freezing on the kinematic parameters of ram spermatozoa sexed by modified protocol with TLR7/8 ligand R848 |
| title_short | Effect of cooling and freezing on the kinematic parameters of ram spermatozoa sexed by modified protocol with TLR7/8 ligand R848 |
| title_sort | effect of cooling and freezing on the kinematic parameters of ram spermatozoa sexed by modified protocol with tlr7 8 ligand r848 |
| topic | ram semen sexing tlr7/8 ligand r848 kinematics cooling freezing |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1450-9156/2024/1450-91562402087Y.pdf |
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