<i>Saussurea involucrata SiLEA5</i> Enhances Tolerance to Drought Stress in <i>Solanum lycopersicum</i>
Drought adversely affects plant growth, which leads to reduced crop yields and exacerbates food insecurity. Late embryogenesis abundant (LEA) proteins are crucial for plants’ responses to abiotic stresses. This research further investigates the role of <i>SiLEA5</i> by utilizing transgen...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-11-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/13/22/3641 |
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| author | Xiaoyan Liu Aowei Li Guanghong Luo Jianbo Zhu |
| author_facet | Xiaoyan Liu Aowei Li Guanghong Luo Jianbo Zhu |
| author_sort | Xiaoyan Liu |
| collection | DOAJ |
| description | Drought adversely affects plant growth, which leads to reduced crop yields and exacerbates food insecurity. Late embryogenesis abundant (LEA) proteins are crucial for plants’ responses to abiotic stresses. This research further investigates the role of <i>SiLEA5</i> by utilizing transgenic tomatoes under drought stress. The expression of <i>SiLEA5</i> was upregulated under drought and abscisic acid (ABA) treatment, resulting in decreased electrolyte leakage and malondialdehyde content, alongside increased levels of osmotic regulators and antioxidant enzyme activity. These biochemical alterations reduce oxidative damage and enhance drought resistance. qRT-PCR analysis revealed the upregulation of ABA signaling genes and key enzymes involved in proline biosynthesis (P5CS) and dehydrin (DHN) synthesis under drought stress. Additionally, overexpression of <i>SiLEA5</i> increased the net photosynthetic rate (Pn) and fruit yield of tomatoes by regulating stomatal density and aperture. These findings suggest that <i>SiLEA5</i> may be a potential target for improving drought tolerance in tomatoes and other crops. |
| format | Article |
| id | doaj-art-4fd7dda439264faabe5fd5372e200cad |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-4fd7dda439264faabe5fd5372e200cad2024-11-26T18:04:40ZengMDPI AGFoods2304-81582024-11-011322364110.3390/foods13223641<i>Saussurea involucrata SiLEA5</i> Enhances Tolerance to Drought Stress in <i>Solanum lycopersicum</i>Xiaoyan Liu0Aowei Li1Guanghong Luo2Jianbo Zhu3Gansu Engineering Technology Research Center for Microalgae, Hexi University, Zhangye 734000, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832000, ChinaGansu Engineering Technology Research Center for Microalgae, Hexi University, Zhangye 734000, ChinaCollege of Life Sciences, Shihezi University, Shihezi 832000, ChinaDrought adversely affects plant growth, which leads to reduced crop yields and exacerbates food insecurity. Late embryogenesis abundant (LEA) proteins are crucial for plants’ responses to abiotic stresses. This research further investigates the role of <i>SiLEA5</i> by utilizing transgenic tomatoes under drought stress. The expression of <i>SiLEA5</i> was upregulated under drought and abscisic acid (ABA) treatment, resulting in decreased electrolyte leakage and malondialdehyde content, alongside increased levels of osmotic regulators and antioxidant enzyme activity. These biochemical alterations reduce oxidative damage and enhance drought resistance. qRT-PCR analysis revealed the upregulation of ABA signaling genes and key enzymes involved in proline biosynthesis (P5CS) and dehydrin (DHN) synthesis under drought stress. Additionally, overexpression of <i>SiLEA5</i> increased the net photosynthetic rate (Pn) and fruit yield of tomatoes by regulating stomatal density and aperture. These findings suggest that <i>SiLEA5</i> may be a potential target for improving drought tolerance in tomatoes and other crops.https://www.mdpi.com/2304-8158/13/22/3641ABADHNdrought resistancestomatal density<i>P5CS</i> |
| spellingShingle | Xiaoyan Liu Aowei Li Guanghong Luo Jianbo Zhu <i>Saussurea involucrata SiLEA5</i> Enhances Tolerance to Drought Stress in <i>Solanum lycopersicum</i> Foods ABA DHN drought resistance stomatal density <i>P5CS</i> |
| title | <i>Saussurea involucrata SiLEA5</i> Enhances Tolerance to Drought Stress in <i>Solanum lycopersicum</i> |
| title_full | <i>Saussurea involucrata SiLEA5</i> Enhances Tolerance to Drought Stress in <i>Solanum lycopersicum</i> |
| title_fullStr | <i>Saussurea involucrata SiLEA5</i> Enhances Tolerance to Drought Stress in <i>Solanum lycopersicum</i> |
| title_full_unstemmed | <i>Saussurea involucrata SiLEA5</i> Enhances Tolerance to Drought Stress in <i>Solanum lycopersicum</i> |
| title_short | <i>Saussurea involucrata SiLEA5</i> Enhances Tolerance to Drought Stress in <i>Solanum lycopersicum</i> |
| title_sort | i saussurea involucrata silea5 i enhances tolerance to drought stress in i solanum lycopersicum i |
| topic | ABA DHN drought resistance stomatal density <i>P5CS</i> |
| url | https://www.mdpi.com/2304-8158/13/22/3641 |
| work_keys_str_mv | AT xiaoyanliu isaussureainvolucratasilea5ienhancestolerancetodroughtstressinisolanumlycopersicumi AT aoweili isaussureainvolucratasilea5ienhancestolerancetodroughtstressinisolanumlycopersicumi AT guanghongluo isaussureainvolucratasilea5ienhancestolerancetodroughtstressinisolanumlycopersicumi AT jianbozhu isaussureainvolucratasilea5ienhancestolerancetodroughtstressinisolanumlycopersicumi |