Elucidating metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions through microbial photo-electrosynthesis
Abstract Reducing greenhouse gas emissions is critical for humanity nowadays, but it can be beneficial by developing engineered systems that valorize CO2 into commodities, thus mimicking nature’s wisdom. Purple phototrophic bacteria (PPB) naturally accept CO2 into their metabolism as a primary redox...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-11-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-024-07188-0 |
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| author | Sara Díaz-Rullo Edreira Ioanna A. Vasiliadou Amanda Prado Juan José Espada Ruddy Wattiez Baptiste Leroy Fernando Martínez Daniel Puyol |
| author_facet | Sara Díaz-Rullo Edreira Ioanna A. Vasiliadou Amanda Prado Juan José Espada Ruddy Wattiez Baptiste Leroy Fernando Martínez Daniel Puyol |
| author_sort | Sara Díaz-Rullo Edreira |
| collection | DOAJ |
| description | Abstract Reducing greenhouse gas emissions is critical for humanity nowadays, but it can be beneficial by developing engineered systems that valorize CO2 into commodities, thus mimicking nature’s wisdom. Purple phototrophic bacteria (PPB) naturally accept CO2 into their metabolism as a primary redox sink system in photo-heterotrophy. Dedicated use of this feature for developing sustainable processes (e.g., through negative-emissions photo-bioelectrosynthesis) requires a deep knowledge of the inherent metabolic mechanisms. This work provides evidence of tuning the PPB metabolic mechanisms upon redox stressing through negative polarization (−0.4 and −0.8 V vs. Ag/AgCl) in photo-bioelectrochemical devices. A mixed PPB-culture upregulates its ability to capture CO2 from organics oxidation through the Calvin-Besson-Bassam cycle and anaplerotic pathways, and the redox imbalance is promoted to polyhydroxyalkanoates production. The ecological relationship of PPB with mutualist bacteria stabilizes the system and opens the door for future development of photo-bioelectrochemical devices focused on CO up-cycling. |
| format | Article |
| id | doaj-art-4fa572dfc35f4fa7b4c8f62f87ad9799 |
| institution | Kabale University |
| issn | 2399-3642 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj-art-4fa572dfc35f4fa7b4c8f62f87ad97992024-11-24T12:39:00ZengNature PortfolioCommunications Biology2399-36422024-11-017111410.1038/s42003-024-07188-0Elucidating metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions through microbial photo-electrosynthesisSara Díaz-Rullo Edreira0Ioanna A. Vasiliadou1Amanda Prado2Juan José Espada3Ruddy Wattiez4Baptiste Leroy5Fernando Martínez6Daniel Puyol7Department of Chemical and Environmental Engineering, High School of Experimental Sciences and Technology, University Rey Juan CarlosDepartment of Environmental Engineering, Democritus University of ThraceDepartment of Automation, Electric Engineering and Electronic Technology, Polytechnic University of CartagenaDepartment of Chemical and Environmental Engineering, High School of Experimental Sciences and Technology, University Rey Juan CarlosLaboratory of Proteomics and Microbiology, Research Institute for Biosciences, University of MonsLaboratory of Proteomics and Microbiology, Research Institute for Biosciences, University of MonsDepartment of Chemical and Environmental Engineering, High School of Experimental Sciences and Technology, University Rey Juan CarlosDepartment of Chemical and Environmental Engineering, High School of Experimental Sciences and Technology, University Rey Juan CarlosAbstract Reducing greenhouse gas emissions is critical for humanity nowadays, but it can be beneficial by developing engineered systems that valorize CO2 into commodities, thus mimicking nature’s wisdom. Purple phototrophic bacteria (PPB) naturally accept CO2 into their metabolism as a primary redox sink system in photo-heterotrophy. Dedicated use of this feature for developing sustainable processes (e.g., through negative-emissions photo-bioelectrosynthesis) requires a deep knowledge of the inherent metabolic mechanisms. This work provides evidence of tuning the PPB metabolic mechanisms upon redox stressing through negative polarization (−0.4 and −0.8 V vs. Ag/AgCl) in photo-bioelectrochemical devices. A mixed PPB-culture upregulates its ability to capture CO2 from organics oxidation through the Calvin-Besson-Bassam cycle and anaplerotic pathways, and the redox imbalance is promoted to polyhydroxyalkanoates production. The ecological relationship of PPB with mutualist bacteria stabilizes the system and opens the door for future development of photo-bioelectrochemical devices focused on CO up-cycling.https://doi.org/10.1038/s42003-024-07188-0 |
| spellingShingle | Sara Díaz-Rullo Edreira Ioanna A. Vasiliadou Amanda Prado Juan José Espada Ruddy Wattiez Baptiste Leroy Fernando Martínez Daniel Puyol Elucidating metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions through microbial photo-electrosynthesis Communications Biology |
| title | Elucidating metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions through microbial photo-electrosynthesis |
| title_full | Elucidating metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions through microbial photo-electrosynthesis |
| title_fullStr | Elucidating metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions through microbial photo-electrosynthesis |
| title_full_unstemmed | Elucidating metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions through microbial photo-electrosynthesis |
| title_short | Elucidating metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions through microbial photo-electrosynthesis |
| title_sort | elucidating metabolic tuning of mixed purple phototrophic bacteria biofilms in photoheterotrophic conditions through microbial photo electrosynthesis |
| url | https://doi.org/10.1038/s42003-024-07188-0 |
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