Facile and controllable hybrid-nanoengineering of MWCNTs/Au@ZIF-8 and AuPt@CeO2 based sandwich electrochemical aptasensor for AFB1 determination in foods and herbs

Herein, a sandwich electrochemical sensing strategy for aflatoxin b1 (AFB1) detection based on hybrid-nanoengineering was presented. First, Au nanoparticle was doped into zeolitic imidazolate framework-8 (ZIF-8) to form Au@ZIF-8 by in-situ growth method, followed by multi-walled carbon nanotubes (MW...

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Main Authors: Liang Guo, Shijin Zhou, Yanju Liu, Huaixia Yang, Mingsan Miao, Wei Gao
Format: Article
Language:English
Published: Elsevier 2024-11-01
Series:Journal of Saudi Chemical Society
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Online Access:http://www.sciencedirect.com/science/article/pii/S1319610324001418
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author Liang Guo
Shijin Zhou
Yanju Liu
Huaixia Yang
Mingsan Miao
Wei Gao
author_facet Liang Guo
Shijin Zhou
Yanju Liu
Huaixia Yang
Mingsan Miao
Wei Gao
author_sort Liang Guo
collection DOAJ
description Herein, a sandwich electrochemical sensing strategy for aflatoxin b1 (AFB1) detection based on hybrid-nanoengineering was presented. First, Au nanoparticle was doped into zeolitic imidazolate framework-8 (ZIF-8) to form Au@ZIF-8 by in-situ growth method, followed by multi-walled carbon nanotubes (MWCNTs) addition to synthesize MWCNTs/Au@ZIF-8 via self-assembly. The structural “confinement effect” of ZIF-8 afforded a microenvironment for Au nanoparticles and SMCNTs in a certain spatial region, giving MWCNTs/Au@ZIF-8 excellent electrochemical property as the substrate material. In addition, Au-Pt bimetallic nanoparticle, which exhibited excellent stability and catalytic activity was loaded on the hollow cerium oxide (CeO2) to form AuPt@CeO2 nanoparticle through one-step aqueous phase reduction. Owning to its high surface-to-volume ratio, satisfied electron transfer efficiency and biocompatibility, massive toluidine blue (TB) and AFB1 antibody (Ab) could be modified on the AuPt@CeO2 to form AuPt@CeO2-Ab-TB, which acted as signal tag for the ultrasensitive assay of AFB1. The proposed electrochemical sensing system exhibited wide detection range (2 × 10-5 − 20 ng/mL) and low detection limit (2.13 fg/mL), which has been successfully applied to AFB1 determination in four real samples. The hybrid nanoengineering presented in this work is an active attempt to prepare high-performance substrate material and signal tag, which provides a new insight for the development of highly sensitive and specific electrochemical sensing systems.
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publishDate 2024-11-01
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series Journal of Saudi Chemical Society
spelling doaj-art-4f98c72e758049cebbca43b46e7a9c502024-12-19T10:52:38ZengElsevierJournal of Saudi Chemical Society1319-61032024-11-01286101946Facile and controllable hybrid-nanoengineering of MWCNTs/Au@ZIF-8 and AuPt@CeO2 based sandwich electrochemical aptasensor for AFB1 determination in foods and herbsLiang Guo0Shijin Zhou1Yanju Liu2Huaixia Yang3Mingsan Miao4Wei Gao5Pharmacy College, Henan University of Chinese Medicine, Zhengzhou 450046, PR ChinaPharmacy College, Henan University of Chinese Medicine, Zhengzhou 450046, PR ChinaPharmacy College, Henan University of Chinese Medicine, Zhengzhou 450046, PR China; Corresponding authors.Pharmacy College, Henan University of Chinese Medicine, Zhengzhou 450046, PR ChinaPharmacy College, Henan University of Chinese Medicine, Zhengzhou 450046, PR China; Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao, Henan Province, PR ChinaPharmacy College, Henan University of Chinese Medicine, Zhengzhou 450046, PR China; Corresponding authors.Herein, a sandwich electrochemical sensing strategy for aflatoxin b1 (AFB1) detection based on hybrid-nanoengineering was presented. First, Au nanoparticle was doped into zeolitic imidazolate framework-8 (ZIF-8) to form Au@ZIF-8 by in-situ growth method, followed by multi-walled carbon nanotubes (MWCNTs) addition to synthesize MWCNTs/Au@ZIF-8 via self-assembly. The structural “confinement effect” of ZIF-8 afforded a microenvironment for Au nanoparticles and SMCNTs in a certain spatial region, giving MWCNTs/Au@ZIF-8 excellent electrochemical property as the substrate material. In addition, Au-Pt bimetallic nanoparticle, which exhibited excellent stability and catalytic activity was loaded on the hollow cerium oxide (CeO2) to form AuPt@CeO2 nanoparticle through one-step aqueous phase reduction. Owning to its high surface-to-volume ratio, satisfied electron transfer efficiency and biocompatibility, massive toluidine blue (TB) and AFB1 antibody (Ab) could be modified on the AuPt@CeO2 to form AuPt@CeO2-Ab-TB, which acted as signal tag for the ultrasensitive assay of AFB1. The proposed electrochemical sensing system exhibited wide detection range (2 × 10-5 − 20 ng/mL) and low detection limit (2.13 fg/mL), which has been successfully applied to AFB1 determination in four real samples. The hybrid nanoengineering presented in this work is an active attempt to prepare high-performance substrate material and signal tag, which provides a new insight for the development of highly sensitive and specific electrochemical sensing systems.http://www.sciencedirect.com/science/article/pii/S1319610324001418Electrochemical sensorAflatoxin b1Hybrid-nanoengineeringSubstrate materialSignal tag
spellingShingle Liang Guo
Shijin Zhou
Yanju Liu
Huaixia Yang
Mingsan Miao
Wei Gao
Facile and controllable hybrid-nanoengineering of MWCNTs/Au@ZIF-8 and AuPt@CeO2 based sandwich electrochemical aptasensor for AFB1 determination in foods and herbs
Journal of Saudi Chemical Society
Electrochemical sensor
Aflatoxin b1
Hybrid-nanoengineering
Substrate material
Signal tag
title Facile and controllable hybrid-nanoengineering of MWCNTs/Au@ZIF-8 and AuPt@CeO2 based sandwich electrochemical aptasensor for AFB1 determination in foods and herbs
title_full Facile and controllable hybrid-nanoengineering of MWCNTs/Au@ZIF-8 and AuPt@CeO2 based sandwich electrochemical aptasensor for AFB1 determination in foods and herbs
title_fullStr Facile and controllable hybrid-nanoengineering of MWCNTs/Au@ZIF-8 and AuPt@CeO2 based sandwich electrochemical aptasensor for AFB1 determination in foods and herbs
title_full_unstemmed Facile and controllable hybrid-nanoengineering of MWCNTs/Au@ZIF-8 and AuPt@CeO2 based sandwich electrochemical aptasensor for AFB1 determination in foods and herbs
title_short Facile and controllable hybrid-nanoengineering of MWCNTs/Au@ZIF-8 and AuPt@CeO2 based sandwich electrochemical aptasensor for AFB1 determination in foods and herbs
title_sort facile and controllable hybrid nanoengineering of mwcnts au zif 8 and aupt ceo2 based sandwich electrochemical aptasensor for afb1 determination in foods and herbs
topic Electrochemical sensor
Aflatoxin b1
Hybrid-nanoengineering
Substrate material
Signal tag
url http://www.sciencedirect.com/science/article/pii/S1319610324001418
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