Ecotype-specific responses of Typha domingensis to microplastics: Antioxidant defense and biochemical adaptations in wetland ecosystems

The inherent ability of plants to withstand microplastic (MP) stress is primarily governed by complex structural and physio-biochemical adaptations. In this study, the MP tolerance mechanisms of six naturally occurring ecotypes of Typha domingensis Pers. were assessed by examining plant pigments, io...

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Main Authors: Muhammad Aqeel, Ali Noman, Saher Nawaz, Noreen Khalid, Muhammad Kashif Irshad, Muhammad Faisal Iqbal, Modhi O. Alotaibi, Motirh Al-Mutairi, Mohamed O. Alshahrani, Nahaa M. Alotaibi, Fahad Mohammed Alzuaibr
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2025-01-01
Series:Environmental Chemistry and Ecotoxicology
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590182625001043
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author Muhammad Aqeel
Ali Noman
Saher Nawaz
Noreen Khalid
Muhammad Kashif Irshad
Muhammad Faisal Iqbal
Modhi O. Alotaibi
Motirh Al-Mutairi
Mohamed O. Alshahrani
Nahaa M. Alotaibi
Fahad Mohammed Alzuaibr
author_facet Muhammad Aqeel
Ali Noman
Saher Nawaz
Noreen Khalid
Muhammad Kashif Irshad
Muhammad Faisal Iqbal
Modhi O. Alotaibi
Motirh Al-Mutairi
Mohamed O. Alshahrani
Nahaa M. Alotaibi
Fahad Mohammed Alzuaibr
author_sort Muhammad Aqeel
collection DOAJ
description The inherent ability of plants to withstand microplastic (MP) stress is primarily governed by complex structural and physio-biochemical adaptations. In this study, the MP tolerance mechanisms of six naturally occurring ecotypes of Typha domingensis Pers. were assessed by examining plant pigments, ionic homeostasis, secondary metabolites, and antioxidant defense responses in MP-contaminated wetlands. Data were analyzed using one-way ANOVA and PCA (n = 6), with significance at P ≤ 0.05. Significant variations were observed among ecotypes in plant growth, physiology, and biochemical responses. Marala Headworks (Eco-6) and River Jhelum (Eco-1) ecotypes displayed the maximum shoot and root biomass, indicating greater tolerance to MPs. Conversely, ecotypes i.e., Aik Drain (Eco-3) and River Ravi (Eco-5) exhibited the lowest biomass, suggesting heightened sensitivity to MP toxicity. Chlorophyll a and b contents varied significantly, with Eco-1 showing the highest and Eco-5 the lowest concentrations. Antioxidant enzyme (SOD, POD, and CAT) activities were significantly elevated in Eco-6, while Eco-5 showed the lowest activity. MP exposure led to increased malondialdehyde (MDA) levels, particularly in Eco-3 and Eco-5, highlighting damages due to oxidative stress. Influence of different MPs in all ecotypes was evident in terms of total soluble sugars and total free amino acids. Mineral ion contents, including sodium, potassium, and nitrogen, also varied significantly among ecotypes after exposure to MP. Heavy metals (Cr, Pb, As, Ni, Cd) and oxidative stress markers correlated positively with MP concentration. Analysis revealed distinct MP polymer compositions and shape distributions among ecotypes. The findings underscore the complex interplay between MPs, heavy metals, and plant physiology, necessitating further research on mitigation strategies for wetland ecosystems.
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spelling doaj-art-4f973f93d69b4fd9ab40e1bdac112e562025-08-20T03:51:53ZengKeAi Communications Co., Ltd.Environmental Chemistry and Ecotoxicology2590-18262025-01-0171518153110.1016/j.enceco.2025.07.008Ecotype-specific responses of Typha domingensis to microplastics: Antioxidant defense and biochemical adaptations in wetland ecosystemsMuhammad Aqeel0Ali Noman1Saher Nawaz2Noreen Khalid3Muhammad Kashif Irshad4Muhammad Faisal Iqbal5Modhi O. Alotaibi6Motirh Al-Mutairi7Mohamed O. Alshahrani8Nahaa M. Alotaibi9Fahad Mohammed Alzuaibr10State Key Laboratory of Herbage Improvement and Grassland Agro-ecosystems (SKLHIGA), College of Ecology, Lanzhou University, Lanzhou 730000, Gansu, PR ChinaDepartment of Botany, Government College University Faisalabad, Pakistan; Corresponding author.Department of Botany, Ghazi University, Dera Ghazi Khan, PakistanDepartment of Botany, Government College Women University Sialkot, Sialkot, PakistanDepartment of Environmental Sciences, Government College University Faisalabad, Pakistan; Department of Environmental and Energy Engineering, Yonsei University, Wonju 26493, Republic of KoreaDepartment of Physics, Riphah International University Faisalabad, Faisalabad 38000, Punjab, PakistanDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia; Environmental and Biomaterial Unit, Natural and Health Sciences Research Center, Princess Nourah bint Abdulrahman University, Riyadh, Saudi ArabiaEnvironmental and Biomaterial Unit, Natural and Health Sciences Research Center, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia; Department of Geography and Environmental Sustainability, College of Humanities and Social Sciences, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaBiology Department, College of Science, King Khalid University, Abha 61421, Saudi ArabiaDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaBiology Department, Faculty of Science, Tabuk University, Tabuk 71491, Saudi ArabiaThe inherent ability of plants to withstand microplastic (MP) stress is primarily governed by complex structural and physio-biochemical adaptations. In this study, the MP tolerance mechanisms of six naturally occurring ecotypes of Typha domingensis Pers. were assessed by examining plant pigments, ionic homeostasis, secondary metabolites, and antioxidant defense responses in MP-contaminated wetlands. Data were analyzed using one-way ANOVA and PCA (n = 6), with significance at P ≤ 0.05. Significant variations were observed among ecotypes in plant growth, physiology, and biochemical responses. Marala Headworks (Eco-6) and River Jhelum (Eco-1) ecotypes displayed the maximum shoot and root biomass, indicating greater tolerance to MPs. Conversely, ecotypes i.e., Aik Drain (Eco-3) and River Ravi (Eco-5) exhibited the lowest biomass, suggesting heightened sensitivity to MP toxicity. Chlorophyll a and b contents varied significantly, with Eco-1 showing the highest and Eco-5 the lowest concentrations. Antioxidant enzyme (SOD, POD, and CAT) activities were significantly elevated in Eco-6, while Eco-5 showed the lowest activity. MP exposure led to increased malondialdehyde (MDA) levels, particularly in Eco-3 and Eco-5, highlighting damages due to oxidative stress. Influence of different MPs in all ecotypes was evident in terms of total soluble sugars and total free amino acids. Mineral ion contents, including sodium, potassium, and nitrogen, also varied significantly among ecotypes after exposure to MP. Heavy metals (Cr, Pb, As, Ni, Cd) and oxidative stress markers correlated positively with MP concentration. Analysis revealed distinct MP polymer compositions and shape distributions among ecotypes. The findings underscore the complex interplay between MPs, heavy metals, and plant physiology, necessitating further research on mitigation strategies for wetland ecosystems.http://www.sciencedirect.com/science/article/pii/S2590182625001043Biomass variationIonic homeostasisStress tolerancePolymersOxidative damage
spellingShingle Muhammad Aqeel
Ali Noman
Saher Nawaz
Noreen Khalid
Muhammad Kashif Irshad
Muhammad Faisal Iqbal
Modhi O. Alotaibi
Motirh Al-Mutairi
Mohamed O. Alshahrani
Nahaa M. Alotaibi
Fahad Mohammed Alzuaibr
Ecotype-specific responses of Typha domingensis to microplastics: Antioxidant defense and biochemical adaptations in wetland ecosystems
Environmental Chemistry and Ecotoxicology
Biomass variation
Ionic homeostasis
Stress tolerance
Polymers
Oxidative damage
title Ecotype-specific responses of Typha domingensis to microplastics: Antioxidant defense and biochemical adaptations in wetland ecosystems
title_full Ecotype-specific responses of Typha domingensis to microplastics: Antioxidant defense and biochemical adaptations in wetland ecosystems
title_fullStr Ecotype-specific responses of Typha domingensis to microplastics: Antioxidant defense and biochemical adaptations in wetland ecosystems
title_full_unstemmed Ecotype-specific responses of Typha domingensis to microplastics: Antioxidant defense and biochemical adaptations in wetland ecosystems
title_short Ecotype-specific responses of Typha domingensis to microplastics: Antioxidant defense and biochemical adaptations in wetland ecosystems
title_sort ecotype specific responses of typha domingensis to microplastics antioxidant defense and biochemical adaptations in wetland ecosystems
topic Biomass variation
Ionic homeostasis
Stress tolerance
Polymers
Oxidative damage
url http://www.sciencedirect.com/science/article/pii/S2590182625001043
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