Thermoacoustic Molecular Imaging of Small Animals
We have designed, constructed, and tested a thermoacoustic computed tomography (TCT) scanner for imaging optical absorption in small animals in three dimensions. The device utilizes pulsed laser irradiation (680–1064 nm) and a unique, 128-element transducer array. We quantified the isotropic spatial...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2003-04-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1162/15353500200303109 |
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| _version_ | 1841563139206807552 |
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| author | Robert A. Kruger William L. Kiser Daniel R. Reinecke Gabe A. Kruger Kathy D. Miller |
| author_facet | Robert A. Kruger William L. Kiser Daniel R. Reinecke Gabe A. Kruger Kathy D. Miller |
| author_sort | Robert A. Kruger |
| collection | DOAJ |
| description | We have designed, constructed, and tested a thermoacoustic computed tomography (TCT) scanner for imaging optical absorption in small animals in three dimensions. The device utilizes pulsed laser irradiation (680–1064 nm) and a unique, 128-element transducer array. We quantified the isotropic spatial resolution of this scanner to be 0.35 mm. We describe a dual-wavelength subtraction technique for isolating optical dyes with TCT. Phantom experiments demonstrate that we can detect 5 fmol of a near-infrared dye (indocyanine green, ICG) in a 1-ML volume using dual-wavelength subtraction. Initial TCT imaging in phantoms and in two sacrificed mice suggests that three-dimensional, optical absorption patterns in small animals can be detected with an order of magnitude better spatial resolution and an order of magnitude better low-contrast detectability in small animals when compared to fluorescence imaging or diffusion optical tomography. |
| format | Article |
| id | doaj-art-4f45d8c964524fe69f4bac5d35950bfd |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2003-04-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-4f45d8c964524fe69f4bac5d35950bfd2025-01-03T00:11:46ZengSAGE PublishingMolecular Imaging1536-01212003-04-01210.1162/1535350020030310910.1162_15353500200303109Thermoacoustic Molecular Imaging of Small AnimalsRobert A. Kruger0William L. Kiser1Daniel R. Reinecke2Gabe A. Kruger3Kathy D. Miller4OptoSonics, Inc., Indianapolis, INOptoSonics, Inc., Indianapolis, INOptoSonics, Inc., Indianapolis, INOptoSonics, Inc., Indianapolis, INIndiana University Medical CenterWe have designed, constructed, and tested a thermoacoustic computed tomography (TCT) scanner for imaging optical absorption in small animals in three dimensions. The device utilizes pulsed laser irradiation (680–1064 nm) and a unique, 128-element transducer array. We quantified the isotropic spatial resolution of this scanner to be 0.35 mm. We describe a dual-wavelength subtraction technique for isolating optical dyes with TCT. Phantom experiments demonstrate that we can detect 5 fmol of a near-infrared dye (indocyanine green, ICG) in a 1-ML volume using dual-wavelength subtraction. Initial TCT imaging in phantoms and in two sacrificed mice suggests that three-dimensional, optical absorption patterns in small animals can be detected with an order of magnitude better spatial resolution and an order of magnitude better low-contrast detectability in small animals when compared to fluorescence imaging or diffusion optical tomography.https://doi.org/10.1162/15353500200303109 |
| spellingShingle | Robert A. Kruger William L. Kiser Daniel R. Reinecke Gabe A. Kruger Kathy D. Miller Thermoacoustic Molecular Imaging of Small Animals Molecular Imaging |
| title | Thermoacoustic Molecular Imaging of Small Animals |
| title_full | Thermoacoustic Molecular Imaging of Small Animals |
| title_fullStr | Thermoacoustic Molecular Imaging of Small Animals |
| title_full_unstemmed | Thermoacoustic Molecular Imaging of Small Animals |
| title_short | Thermoacoustic Molecular Imaging of Small Animals |
| title_sort | thermoacoustic molecular imaging of small animals |
| url | https://doi.org/10.1162/15353500200303109 |
| work_keys_str_mv | AT robertakruger thermoacousticmolecularimagingofsmallanimals AT williamlkiser thermoacousticmolecularimagingofsmallanimals AT danielrreinecke thermoacousticmolecularimagingofsmallanimals AT gabeakruger thermoacousticmolecularimagingofsmallanimals AT kathydmiller thermoacousticmolecularimagingofsmallanimals |