EFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATION
Abstract. The aim of study was to compare the influence of lipopolysaccharide (LPS) component from Gram-negative bacteria (E. coli 055:B5), and a lysate of Gram-positive bacterium (Streptococcus pyogenes, type M1, strain 40/58) upon transendothelial migration rates of monocyte-like cells (THP-1 stra...
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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| Series: | Медицинская иммунология |
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| Online Access: | https://www.mimmun.ru/mimmun/article/view/271 |
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| author | E. P. Starikova D. I. Sokolov L. A. Burova S. A. Sel’kov I. S. Freidlin |
| author_facet | E. P. Starikova D. I. Sokolov L. A. Burova S. A. Sel’kov I. S. Freidlin |
| author_sort | E. P. Starikova |
| collection | DOAJ |
| description | Abstract. The aim of study was to compare the influence of lipopolysaccharide (LPS) component from Gram-negative bacteria (E. coli 055:B5), and a lysate of Gram-positive bacterium (Streptococcus pyogenes, type M1, strain 40/58) upon transendothelial migration rates of monocyte-like cells (THP-1 strain). Both LPS and lysate of Streptococcus pyogenes acted as chemoattractants for THP-1 cells. he studied components of Streptococcus pyogenes proved to be more active stimulants of transendothelial THP-1 cell migration, than LPS from E. coli. During spontaneous transmigration of THP-1 cells through a monolayer of endothelial cells, augmented levels of chemokines (RANTES, MCP-1, IL-8, IP-10) were noticed, that were more pronounced in presence of LPS. Upon spontaneous transmigration of THP-1 cells through endothelial monolayer, the levels of proinflammatory cytokines (TNFα, IL-1β and IL-6) in cultural medium were found to be rather low. The transmigration-associated secretion of these cytokines increased in presence of LPS and Streptococcus pyogenes lysate. Incubation with these bacterial constituents did increase cytokine levels both in monoculture of THP-1 cells and in transmigration model. Our results suggest that the levels of THP-1 transendothelial migration depend mainly on activation of monocyte-like cells influenced by PRR-ligands from Streptococcus pyogenes lysate. (Med. Immunol., vol. 10, N 6, pp 571-576). |
| format | Article |
| id | doaj-art-4f0c7fd8e9f745e1a8d494f1ada5c91f |
| institution | Kabale University |
| issn | 1563-0625 2313-741X |
| language | Russian |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj-art-4f0c7fd8e9f745e1a8d494f1ada5c91f2025-08-20T03:44:07ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-0110657157610.15789/1563-0625-2008-6-571-576268EFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATIONE. P. Starikova0D. I. Sokolov1L. A. Burova2S. A. Sel’kov3I. S. Freidlin4ГУ НИИ экспериментальной медицины РАМН, Санкт-ПетербургГУ НИИ акушерства и гинекологии им. Д.О. Отта, Санкт-ПетербургГУ НИИ экспериментальной медицины РАМН, Санкт-ПетербургГУ НИИ акушерства и гинекологии им. Д.О. Отта, Санкт-ПетербургГУ НИИ экспериментальной медицины РАМН, Санкт-ПетербургAbstract. The aim of study was to compare the influence of lipopolysaccharide (LPS) component from Gram-negative bacteria (E. coli 055:B5), and a lysate of Gram-positive bacterium (Streptococcus pyogenes, type M1, strain 40/58) upon transendothelial migration rates of monocyte-like cells (THP-1 strain). Both LPS and lysate of Streptococcus pyogenes acted as chemoattractants for THP-1 cells. he studied components of Streptococcus pyogenes proved to be more active stimulants of transendothelial THP-1 cell migration, than LPS from E. coli. During spontaneous transmigration of THP-1 cells through a monolayer of endothelial cells, augmented levels of chemokines (RANTES, MCP-1, IL-8, IP-10) were noticed, that were more pronounced in presence of LPS. Upon spontaneous transmigration of THP-1 cells through endothelial monolayer, the levels of proinflammatory cytokines (TNFα, IL-1β and IL-6) in cultural medium were found to be rather low. The transmigration-associated secretion of these cytokines increased in presence of LPS and Streptococcus pyogenes lysate. Incubation with these bacterial constituents did increase cytokine levels both in monoculture of THP-1 cells and in transmigration model. Our results suggest that the levels of THP-1 transendothelial migration depend mainly on activation of monocyte-like cells influenced by PRR-ligands from Streptococcus pyogenes lysate. (Med. Immunol., vol. 10, N 6, pp 571-576).https://www.mimmun.ru/mimmun/article/view/271transendothelial migrationmonocytescytokineschemokinesprrrantesmcp-1il-8ip-10tnfαil-1βil-6 |
| spellingShingle | E. P. Starikova D. I. Sokolov L. A. Burova S. A. Sel’kov I. S. Freidlin EFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATION Медицинская иммунология transendothelial migration monocytes cytokines chemokines prr rantes mcp-1 il-8 ip-10 tnfα il-1β il-6 |
| title | EFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATION |
| title_full | EFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATION |
| title_fullStr | EFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATION |
| title_full_unstemmed | EFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATION |
| title_short | EFFECTS OF BACTERIAL LIGANDS OF PATTERN-RECOGNIZING RECEPTORS (PRR) ON MONOCYTE-LIKE THP-1 CELLS UPON THEIR TRANSENDOTHELIAL MIGRATION |
| title_sort | effects of bacterial ligands of pattern recognizing receptors prr on monocyte like thp 1 cells upon their transendothelial migration |
| topic | transendothelial migration monocytes cytokines chemokines prr rantes mcp-1 il-8 ip-10 tnfα il-1β il-6 |
| url | https://www.mimmun.ru/mimmun/article/view/271 |
| work_keys_str_mv | AT epstarikova effectsofbacterialligandsofpatternrecognizingreceptorsprronmonocytelikethp1cellsupontheirtransendothelialmigration AT disokolov effectsofbacterialligandsofpatternrecognizingreceptorsprronmonocytelikethp1cellsupontheirtransendothelialmigration AT laburova effectsofbacterialligandsofpatternrecognizingreceptorsprronmonocytelikethp1cellsupontheirtransendothelialmigration AT saselkov effectsofbacterialligandsofpatternrecognizingreceptorsprronmonocytelikethp1cellsupontheirtransendothelialmigration AT isfreidlin effectsofbacterialligandsofpatternrecognizingreceptorsprronmonocytelikethp1cellsupontheirtransendothelialmigration |