End-point RPA-CRISPR/Cas12a-based detection of Enterocytozoon bieneusi nucleic acid: rapid, sensitive and specific

End-point RPA-CRISPR/Cas12a-based detection of Enterocytozoon bieneusi nucleic acid: rapid, sensitive and specific

Abstract Enterocytozoon bieneusi is a common species of microsporidia that infects humans and animals. Current methods for detecting E. bieneusi infections have trade-offs in sensitivity, specificity, simplicity, cost and speed and are thus unacceptable for clinical application. We tested the effect...

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Bibliographic Details
Main Authors: Yilin Wang, Fuchang Yu, Kaihui Zhang, Ke Shi, Yuancai Chen, Junqiang Li, Xiaoying Li, Longxian Zhang
Format: Article
Language:English
Published: BMC 2024-11-01
Series:BMC Veterinary Research
Subjects:
Enterocytozoon bieneusi
Recombinase polymerase amplification
CRISPR/Cas12a
Visualized detection
On-site detection
Online Access:https://doi.org/10.1186/s12917-024-04391-3
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