Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells
Primordial germ cells (PGCs) play a crucial role in transmitting genetic information to the next-generation. In chickens, genetically edited PGCs can be propagated in vitro and subsequently transplanted into recipient embryos to produce offspring with desired genetic traits. However, during early em...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Genome Editing |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgeed.2024.1512108/full |
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| author | Yuzhou Gu Yuzhou Gu Yuzhou Gu Kexin Wu Kexin Wu Kexin Wu Bowen Niu Bowen Niu Bowen Niu Zhiting Wang Zhiting Wang Zhiting Wang Yuchen Jie Yuchen Jie Yuchen Jie Zixuan Fan Zixuan Fan Zixuan Fan Junying Li Junying Li Congjiao Sun Congjiao Sun Congjiao Sun Zhuo-Cheng Hou Zhuo-Cheng Hou Zhuo-Cheng Hou Li-Wa Shao Li-Wa Shao Li-Wa Shao |
| author_facet | Yuzhou Gu Yuzhou Gu Yuzhou Gu Kexin Wu Kexin Wu Kexin Wu Bowen Niu Bowen Niu Bowen Niu Zhiting Wang Zhiting Wang Zhiting Wang Yuchen Jie Yuchen Jie Yuchen Jie Zixuan Fan Zixuan Fan Zixuan Fan Junying Li Junying Li Congjiao Sun Congjiao Sun Congjiao Sun Zhuo-Cheng Hou Zhuo-Cheng Hou Zhuo-Cheng Hou Li-Wa Shao Li-Wa Shao Li-Wa Shao |
| author_sort | Yuzhou Gu |
| collection | DOAJ |
| description | Primordial germ cells (PGCs) play a crucial role in transmitting genetic information to the next-generation. In chickens, genetically edited PGCs can be propagated in vitro and subsequently transplanted into recipient embryos to produce offspring with desired genetic traits. However, during early embryogenesis, the effects of external conditions on PGC migration through the vascular system to the gonads have yet to be explored, which may affect the efficiency of preparing gene-edited chickens. In this study, we investigated the effects of hyperthermia on the biological characteristics and migration of chicken PGCs. A gonad-derived PGC line of White Leghorn (WLH) chicken was established and verified through PAS staining and immunofluorescence of PGC-specific proteins. To visually observe PGC migration in vivo, GFP-positive PGCs were prepared and locations of chimeras were validated. Cell viability, glycogen granule contents, and mRNA expression levels of pluripotency markers (NANOG and POUV), germ cell-specific markers (DAZL and CVH), and telomerase reverse transcriptase (TERT) were reduced in PGCs cultured under high temperatures (43°C for 12, 24, and 48 h). After the heat treatment of donor PGCs (43°C) or recipient embryos (39.5°C), GFP-positive PGCs in gonads were rarely observed. Taken together, our results underscore the negative effects of hyperthermia on the biological characteristics and migration of chicken PGCs, which provides valuable insights for the implementation of PGC-based gene editing techniques in chickens. |
| format | Article |
| id | doaj-art-4eba8ac4f4824209b42e4bf7cb7f9fac |
| institution | Kabale University |
| issn | 2673-3439 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genome Editing |
| spelling | doaj-art-4eba8ac4f4824209b42e4bf7cb7f9fac2025-01-08T06:12:00ZengFrontiers Media S.A.Frontiers in Genome Editing2673-34392025-01-01610.3389/fgeed.2024.15121081512108Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cellsYuzhou Gu0Yuzhou Gu1Yuzhou Gu2Kexin Wu3Kexin Wu4Kexin Wu5Bowen Niu6Bowen Niu7Bowen Niu8Zhiting Wang9Zhiting Wang10Zhiting Wang11Yuchen Jie12Yuchen Jie13Yuchen Jie14Zixuan Fan15Zixuan Fan16Zixuan Fan17Junying Li18Junying Li19Congjiao Sun20Congjiao Sun21Congjiao Sun22Zhuo-Cheng Hou23Zhuo-Cheng Hou24Zhuo-Cheng Hou25Li-Wa Shao26Li-Wa Shao27Li-Wa Shao28State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, ChinaNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, ChinaNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, ChinaNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, ChinaNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, ChinaNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, ChinaNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, ChinaNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, ChinaNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, ChinaNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, ChinaState Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing, ChinaNational Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, ChinaFrontiers Science Center for Molecular Design Breeding (MOE), China Agricultural University, Beijing, ChinaPrimordial germ cells (PGCs) play a crucial role in transmitting genetic information to the next-generation. In chickens, genetically edited PGCs can be propagated in vitro and subsequently transplanted into recipient embryos to produce offspring with desired genetic traits. However, during early embryogenesis, the effects of external conditions on PGC migration through the vascular system to the gonads have yet to be explored, which may affect the efficiency of preparing gene-edited chickens. In this study, we investigated the effects of hyperthermia on the biological characteristics and migration of chicken PGCs. A gonad-derived PGC line of White Leghorn (WLH) chicken was established and verified through PAS staining and immunofluorescence of PGC-specific proteins. To visually observe PGC migration in vivo, GFP-positive PGCs were prepared and locations of chimeras were validated. Cell viability, glycogen granule contents, and mRNA expression levels of pluripotency markers (NANOG and POUV), germ cell-specific markers (DAZL and CVH), and telomerase reverse transcriptase (TERT) were reduced in PGCs cultured under high temperatures (43°C for 12, 24, and 48 h). After the heat treatment of donor PGCs (43°C) or recipient embryos (39.5°C), GFP-positive PGCs in gonads were rarely observed. Taken together, our results underscore the negative effects of hyperthermia on the biological characteristics and migration of chicken PGCs, which provides valuable insights for the implementation of PGC-based gene editing techniques in chickens.https://www.frontiersin.org/articles/10.3389/fgeed.2024.1512108/fullgene-edited chickenprimordial germ cellhyperthermiacell biological characteristicsmigration |
| spellingShingle | Yuzhou Gu Yuzhou Gu Yuzhou Gu Kexin Wu Kexin Wu Kexin Wu Bowen Niu Bowen Niu Bowen Niu Zhiting Wang Zhiting Wang Zhiting Wang Yuchen Jie Yuchen Jie Yuchen Jie Zixuan Fan Zixuan Fan Zixuan Fan Junying Li Junying Li Congjiao Sun Congjiao Sun Congjiao Sun Zhuo-Cheng Hou Zhuo-Cheng Hou Zhuo-Cheng Hou Li-Wa Shao Li-Wa Shao Li-Wa Shao Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells Frontiers in Genome Editing gene-edited chicken primordial germ cell hyperthermia cell biological characteristics migration |
| title | Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells |
| title_full | Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells |
| title_fullStr | Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells |
| title_full_unstemmed | Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells |
| title_short | Hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells |
| title_sort | hyperthermia suppresses the biological characteristics and migration of chicken primordial germ cells |
| topic | gene-edited chicken primordial germ cell hyperthermia cell biological characteristics migration |
| url | https://www.frontiersin.org/articles/10.3389/fgeed.2024.1512108/full |
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