Characterization of a Natural Accession of <i>Elymus sibiricus</i> with <i>In Situ</i> Hybridization and Agronomic Evaluation
<i>Elymus sibiricus</i>, valued for its perennial nature, broad adaptability, strong cold tolerance, and high economic value in forage production, plays a crucial role in combating grassland degradation, desertification, and salinization. Using morphological and cytogenetic methods, this...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/1/75 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841549066273554432 |
|---|---|
| author | Yizhuo Liu Jiarui Ding Chunfei Wu Weiwei Song Xinyu Zhao Haibin Zhao Yunfeng Qu Hui Jin Rui Zhang Mingyao Li Xinyu Yan Liangyu Zhu Yaqi Bao Dianhao Liu Xinling Li Lei Cui Hongjie Li Yanming Zhang |
| author_facet | Yizhuo Liu Jiarui Ding Chunfei Wu Weiwei Song Xinyu Zhao Haibin Zhao Yunfeng Qu Hui Jin Rui Zhang Mingyao Li Xinyu Yan Liangyu Zhu Yaqi Bao Dianhao Liu Xinling Li Lei Cui Hongjie Li Yanming Zhang |
| author_sort | Yizhuo Liu |
| collection | DOAJ |
| description | <i>Elymus sibiricus</i>, valued for its perennial nature, broad adaptability, strong cold tolerance, and high economic value in forage production, plays a crucial role in combating grassland degradation, desertification, and salinization. Using morphological and cytogenetic methods, this study evaluated the cold tolerance, post-harvest regeneration capacity, and perennial characteristics of the <i>E. sibiricus</i> accession 20HSC-Z9 in the Harbin region of China from 2020 to 2023. This accession exhibited a germination rate of over 90% and a 100% green-up rate, with purple coleoptiles indicating its strong cold tolerance. Over the three growing seasons, 20HSC-Z9 maintained stable green-up and regeneration rates, confirming its perennial nature. Morphologically, 20HSC-Z9 had an average tiller count ranging from 56 to 74, similar to that of the control accession 20HSC-ES, and its plant height was significantly lower than that of 20HSC-IWG. Furthermore, 20HSC-Z9 produced over 100 grains per spike, with a seed setting rate exceeding 90%, and a thousand-grain weight comparable to that of 20HSC-IWG. The grain protein content of 20HSC-Z9 reached a maximum of 21.19%, greater than that of the control accessions (15.6% and 18.5%). Chromosome composition analysis, using sequential multicolor genomic <i>in situ</i> hybridization and multicolor fluorescence <i>in situ</i> hybridization, confirmed the StStHH genomic constitution of 20HSC-Z9 and revealed translocations between the St and H subgenome chromosomes. These results suggest that 20HSC-Z9 has significant potential as a new perennial forage grass germplasm for cold regions, suitable for further domestication and breeding efforts. |
| format | Article |
| id | doaj-art-4e7b72a3098e4dd7a7a975695e50c45f |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-4e7b72a3098e4dd7a7a975695e50c45f2025-01-10T13:19:40ZengMDPI AGPlants2223-77472024-12-011417510.3390/plants14010075Characterization of a Natural Accession of <i>Elymus sibiricus</i> with <i>In Situ</i> Hybridization and Agronomic EvaluationYizhuo Liu0Jiarui Ding1Chunfei Wu2Weiwei Song3Xinyu Zhao4Haibin Zhao5Yunfeng Qu6Hui Jin7Rui Zhang8Mingyao Li9Xinyu Yan10Liangyu Zhu11Yaqi Bao12Dianhao Liu13Xinling Li14Lei Cui15Hongjie Li16Yanming Zhang17Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin 150025, ChinaKey Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin 150025, ChinaKey Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin 150025, ChinaKey Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin 150025, ChinaKey Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin 150025, ChinaInstitute of Forage and Grassland Sciences, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaInstitute of Biotechnology, Xianghu Laboratory, Hangzhou 311200, ChinaInstitute of Forage and Grassland Sciences, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaInstitute of Forage and Grassland Sciences, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, ChinaKey Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin 150025, ChinaKey Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin 150025, ChinaKey Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin 150025, ChinaKey Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin 150025, ChinaKey Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin 150025, ChinaKey Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin 150025, ChinaCollege of Agriculture, Shanxi Agricultural University, Taiyuan 030031, ChinaInstitute of Biotechnology, Xianghu Laboratory, Hangzhou 311200, ChinaKey Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin 150025, China<i>Elymus sibiricus</i>, valued for its perennial nature, broad adaptability, strong cold tolerance, and high economic value in forage production, plays a crucial role in combating grassland degradation, desertification, and salinization. Using morphological and cytogenetic methods, this study evaluated the cold tolerance, post-harvest regeneration capacity, and perennial characteristics of the <i>E. sibiricus</i> accession 20HSC-Z9 in the Harbin region of China from 2020 to 2023. This accession exhibited a germination rate of over 90% and a 100% green-up rate, with purple coleoptiles indicating its strong cold tolerance. Over the three growing seasons, 20HSC-Z9 maintained stable green-up and regeneration rates, confirming its perennial nature. Morphologically, 20HSC-Z9 had an average tiller count ranging from 56 to 74, similar to that of the control accession 20HSC-ES, and its plant height was significantly lower than that of 20HSC-IWG. Furthermore, 20HSC-Z9 produced over 100 grains per spike, with a seed setting rate exceeding 90%, and a thousand-grain weight comparable to that of 20HSC-IWG. The grain protein content of 20HSC-Z9 reached a maximum of 21.19%, greater than that of the control accessions (15.6% and 18.5%). Chromosome composition analysis, using sequential multicolor genomic <i>in situ</i> hybridization and multicolor fluorescence <i>in situ</i> hybridization, confirmed the StStHH genomic constitution of 20HSC-Z9 and revealed translocations between the St and H subgenome chromosomes. These results suggest that 20HSC-Z9 has significant potential as a new perennial forage grass germplasm for cold regions, suitable for further domestication and breeding efforts.https://www.mdpi.com/2223-7747/14/1/75<i>Elymus</i>smGISHkaryotypechromosomecold-hardiness |
| spellingShingle | Yizhuo Liu Jiarui Ding Chunfei Wu Weiwei Song Xinyu Zhao Haibin Zhao Yunfeng Qu Hui Jin Rui Zhang Mingyao Li Xinyu Yan Liangyu Zhu Yaqi Bao Dianhao Liu Xinling Li Lei Cui Hongjie Li Yanming Zhang Characterization of a Natural Accession of <i>Elymus sibiricus</i> with <i>In Situ</i> Hybridization and Agronomic Evaluation Plants <i>Elymus</i> smGISH karyotype chromosome cold-hardiness |
| title | Characterization of a Natural Accession of <i>Elymus sibiricus</i> with <i>In Situ</i> Hybridization and Agronomic Evaluation |
| title_full | Characterization of a Natural Accession of <i>Elymus sibiricus</i> with <i>In Situ</i> Hybridization and Agronomic Evaluation |
| title_fullStr | Characterization of a Natural Accession of <i>Elymus sibiricus</i> with <i>In Situ</i> Hybridization and Agronomic Evaluation |
| title_full_unstemmed | Characterization of a Natural Accession of <i>Elymus sibiricus</i> with <i>In Situ</i> Hybridization and Agronomic Evaluation |
| title_short | Characterization of a Natural Accession of <i>Elymus sibiricus</i> with <i>In Situ</i> Hybridization and Agronomic Evaluation |
| title_sort | characterization of a natural accession of i elymus sibiricus i with i in situ i hybridization and agronomic evaluation |
| topic | <i>Elymus</i> smGISH karyotype chromosome cold-hardiness |
| url | https://www.mdpi.com/2223-7747/14/1/75 |
| work_keys_str_mv | AT yizhuoliu characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation AT jiaruiding characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation AT chunfeiwu characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation AT weiweisong characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation AT xinyuzhao characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation AT haibinzhao characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation AT yunfengqu characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation AT huijin characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation AT ruizhang characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation AT mingyaoli characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation AT xinyuyan characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation AT liangyuzhu characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation AT yaqibao characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation AT dianhaoliu characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation AT xinlingli characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation AT leicui characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation AT hongjieli characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation AT yanmingzhang characterizationofanaturalaccessionofielymussibiricusiwithiinsituihybridizationandagronomicevaluation |