Characterization of a Natural Accession of <i>Elymus sibiricus</i> with <i>In Situ</i> Hybridization and Agronomic Evaluation

Characterization of a Natural Accession of <i>Elymus sibiricus</i> with <i>In Situ</i> Hybridization and Agronomic Evaluation

<i>Elymus sibiricus</i>, valued for its perennial nature, broad adaptability, strong cold tolerance, and high economic value in forage production, plays a crucial role in combating grassland degradation, desertification, and salinization. Using morphological and cytogenetic methods, this...

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Main Authors: Yizhuo Liu, Jiarui Ding, Chunfei Wu, Weiwei Song, Xinyu Zhao, Haibin Zhao, Yunfeng Qu, Hui Jin, Rui Zhang, Mingyao Li, Xinyu Yan, Liangyu Zhu, Yaqi Bao, Dianhao Liu, Xinling Li, Lei Cui, Hongjie Li, Yanming Zhang
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Plants
Subjects:
<i>Elymus</i>
smGISH
karyotype
chromosome
cold-hardiness
Online Access:https://www.mdpi.com/2223-7747/14/1/75
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Summary:<i>Elymus sibiricus</i>, valued for its perennial nature, broad adaptability, strong cold tolerance, and high economic value in forage production, plays a crucial role in combating grassland degradation, desertification, and salinization. Using morphological and cytogenetic methods, this study evaluated the cold tolerance, post-harvest regeneration capacity, and perennial characteristics of the <i>E. sibiricus</i> accession 20HSC-Z9 in the Harbin region of China from 2020 to 2023. This accession exhibited a germination rate of over 90% and a 100% green-up rate, with purple coleoptiles indicating its strong cold tolerance. Over the three growing seasons, 20HSC-Z9 maintained stable green-up and regeneration rates, confirming its perennial nature. Morphologically, 20HSC-Z9 had an average tiller count ranging from 56 to 74, similar to that of the control accession 20HSC-ES, and its plant height was significantly lower than that of 20HSC-IWG. Furthermore, 20HSC-Z9 produced over 100 grains per spike, with a seed setting rate exceeding 90%, and a thousand-grain weight comparable to that of 20HSC-IWG. The grain protein content of 20HSC-Z9 reached a maximum of 21.19%, greater than that of the control accessions (15.6% and 18.5%). Chromosome composition analysis, using sequential multicolor genomic <i>in situ</i> hybridization and multicolor fluorescence <i>in situ</i> hybridization, confirmed the StStHH genomic constitution of 20HSC-Z9 and revealed translocations between the St and H subgenome chromosomes. These results suggest that 20HSC-Z9 has significant potential as a new perennial forage grass germplasm for cold regions, suitable for further domestication and breeding efforts.
ISSN:2223-7747

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