Establishment of an induced pluripotent cell line (ABCRIi001-A) from an elderly female for ageing research

Establishment of an induced pluripotent cell line (ABCRIi001-A) from an elderly female for ageing research

Human induced pluripotent stem cells (hiPSCs) hold promises to model and understand human diseases, including those associated with ageing. Here, we describe ABCRIi001-A, a hiPSC line generated from peripheral blood mononuclear cells (PBMCs) of a 79-year-old female enrolled in a study for developmen...

Full description

Saved in:
Bibliographic Details
Main Authors: Filipa Esteves, David Brito, Ana Teresa Rajado, Nádia Silva, Joana Apolónio, Vânia Palma Roberto, Raquel P. Andrade, Sofia Calado, Maria Leonor Faleiro, Carlos Matos, Nuno Marques, Ana Marreiros, Hipólito Nzwalo, Sandra Pais, Isabel Palmeirim, Sónia Simãoa, Natércia Joaquim, Rui Miranda, António Pêgas, Daniela Marques Raposo, Ana Sardo, Inês Araújo, Clévio Nóbrega, Pedro Castelo-Branco, José Bragança
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506124002745
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Human induced pluripotent stem cells (hiPSCs) hold promises to model and understand human diseases, including those associated with ageing. Here, we describe ABCRIi001-A, a hiPSC line generated from peripheral blood mononuclear cells (PBMCs) of a 79-year-old female enrolled in a study for development of an ageing score (ALFA Score). PBMCs were reprogrammed using three Sendai virus-based reprogramming vectors (hKOS, hc-Myc, and hKlf4). ABCRIi001-A showed normal morphology and karyotype, viral clearance, absence of genomic aberrations, and their pluripotency was confirmed by expression of pluripotency-related markers and their ability to differentiate into the three germ layers. ABCRIi001-A is valuable for ageing-related studies.
ISSN:1873-5061

Similar Items