A novel neutralizing monoclonal antibody recognizes a linear antigenic epitope of the spike protein of swine acute diarrhoea syndrome coronavirus
Abstract Swine acute diarrhoea syndrome coronavirus (SADS-CoV) causes vomiting, severe diarrhoea and death in newborn piglets. The spike (S) protein plays a crucial role in promoting virus invasion and inducing neutralizing antibody production. In this study, the extracellular region of the S protei...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-11-01
|
| Series: | Virology Journal |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12985-024-02562-0 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846172236814221312 |
|---|---|
| author | Lin Zhang Hui-Zhen Liu Yuexiao Lian Yujun Zhu Miaoli Wu Jianbo Liu Feng Cong |
| author_facet | Lin Zhang Hui-Zhen Liu Yuexiao Lian Yujun Zhu Miaoli Wu Jianbo Liu Feng Cong |
| author_sort | Lin Zhang |
| collection | DOAJ |
| description | Abstract Swine acute diarrhoea syndrome coronavirus (SADS-CoV) causes vomiting, severe diarrhoea and death in newborn piglets. The spike (S) protein plays a crucial role in promoting virus invasion and inducing neutralizing antibody production. In this study, the extracellular region of the S protein was used as an immunogen to immunize BALB/c mice. After immunization, B cells were collected, fused with SP2/0 myeloma cells, cultured and subcloned, and a cell line capable of secreting neutralizing antibodies was obtained and named as 5D6. Additionally, it was determined that the 5D6 mAb could be used as the primary antibody for western blotting and indirect immunofluorescence assay (IFA) to detect SADS-CoV. Further studies indicated that the 5D6 mAb binds to the 136STSHAAD142 motif, which located in the N-terminal domain (NTD) of the spike protein. This result suggested that the NTD of the S protein can induce the production of neutralizing antibodies. Amino acid sequence alignment revealed that the epitope of the 5D6 mAb was conserved among SADS-CoV strains. This study helps elucidate the S protein function of SADS-CoV, and the 5D6 mAb may be used to develop diagnostic and treatment tools for detecting SADS-CoV infection. |
| format | Article |
| id | doaj-art-4e473b853cb24e7f8ab3dfb5edb744f4 |
| institution | Kabale University |
| issn | 1743-422X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Virology Journal |
| spelling | doaj-art-4e473b853cb24e7f8ab3dfb5edb744f42024-11-10T12:06:43ZengBMCVirology Journal1743-422X2024-11-0121111110.1186/s12985-024-02562-0A novel neutralizing monoclonal antibody recognizes a linear antigenic epitope of the spike protein of swine acute diarrhoea syndrome coronavirusLin Zhang0Hui-Zhen Liu1Yuexiao Lian2Yujun Zhu3Miaoli Wu4Jianbo Liu5Feng Cong6Guangdong Laboratory Animals Monitoring InstituteGuangdong Laboratory Animals Monitoring InstituteGuangdong Laboratory Animals Monitoring InstituteGuangdong Laboratory Animals Monitoring InstituteGuangdong Laboratory Animals Monitoring InstituteState Key Laboratory of Respiratory Disease, CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong Provincial Key Laboratory of Biocomputing, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesGuangdong Laboratory Animals Monitoring InstituteAbstract Swine acute diarrhoea syndrome coronavirus (SADS-CoV) causes vomiting, severe diarrhoea and death in newborn piglets. The spike (S) protein plays a crucial role in promoting virus invasion and inducing neutralizing antibody production. In this study, the extracellular region of the S protein was used as an immunogen to immunize BALB/c mice. After immunization, B cells were collected, fused with SP2/0 myeloma cells, cultured and subcloned, and a cell line capable of secreting neutralizing antibodies was obtained and named as 5D6. Additionally, it was determined that the 5D6 mAb could be used as the primary antibody for western blotting and indirect immunofluorescence assay (IFA) to detect SADS-CoV. Further studies indicated that the 5D6 mAb binds to the 136STSHAAD142 motif, which located in the N-terminal domain (NTD) of the spike protein. This result suggested that the NTD of the S protein can induce the production of neutralizing antibodies. Amino acid sequence alignment revealed that the epitope of the 5D6 mAb was conserved among SADS-CoV strains. This study helps elucidate the S protein function of SADS-CoV, and the 5D6 mAb may be used to develop diagnostic and treatment tools for detecting SADS-CoV infection.https://doi.org/10.1186/s12985-024-02562-0Swine acute diarrhoea syndrome coronavirus (SADS-CoV)Neutralizing antibodiesAntigenic epitope |
| spellingShingle | Lin Zhang Hui-Zhen Liu Yuexiao Lian Yujun Zhu Miaoli Wu Jianbo Liu Feng Cong A novel neutralizing monoclonal antibody recognizes a linear antigenic epitope of the spike protein of swine acute diarrhoea syndrome coronavirus Virology Journal Swine acute diarrhoea syndrome coronavirus (SADS-CoV) Neutralizing antibodies Antigenic epitope |
| title | A novel neutralizing monoclonal antibody recognizes a linear antigenic epitope of the spike protein of swine acute diarrhoea syndrome coronavirus |
| title_full | A novel neutralizing monoclonal antibody recognizes a linear antigenic epitope of the spike protein of swine acute diarrhoea syndrome coronavirus |
| title_fullStr | A novel neutralizing monoclonal antibody recognizes a linear antigenic epitope of the spike protein of swine acute diarrhoea syndrome coronavirus |
| title_full_unstemmed | A novel neutralizing monoclonal antibody recognizes a linear antigenic epitope of the spike protein of swine acute diarrhoea syndrome coronavirus |
| title_short | A novel neutralizing monoclonal antibody recognizes a linear antigenic epitope of the spike protein of swine acute diarrhoea syndrome coronavirus |
| title_sort | novel neutralizing monoclonal antibody recognizes a linear antigenic epitope of the spike protein of swine acute diarrhoea syndrome coronavirus |
| topic | Swine acute diarrhoea syndrome coronavirus (SADS-CoV) Neutralizing antibodies Antigenic epitope |
| url | https://doi.org/10.1186/s12985-024-02562-0 |
| work_keys_str_mv | AT linzhang anovelneutralizingmonoclonalantibodyrecognizesalinearantigenicepitopeofthespikeproteinofswineacutediarrhoeasyndromecoronavirus AT huizhenliu anovelneutralizingmonoclonalantibodyrecognizesalinearantigenicepitopeofthespikeproteinofswineacutediarrhoeasyndromecoronavirus AT yuexiaolian anovelneutralizingmonoclonalantibodyrecognizesalinearantigenicepitopeofthespikeproteinofswineacutediarrhoeasyndromecoronavirus AT yujunzhu anovelneutralizingmonoclonalantibodyrecognizesalinearantigenicepitopeofthespikeproteinofswineacutediarrhoeasyndromecoronavirus AT miaoliwu anovelneutralizingmonoclonalantibodyrecognizesalinearantigenicepitopeofthespikeproteinofswineacutediarrhoeasyndromecoronavirus AT jianboliu anovelneutralizingmonoclonalantibodyrecognizesalinearantigenicepitopeofthespikeproteinofswineacutediarrhoeasyndromecoronavirus AT fengcong anovelneutralizingmonoclonalantibodyrecognizesalinearantigenicepitopeofthespikeproteinofswineacutediarrhoeasyndromecoronavirus AT linzhang novelneutralizingmonoclonalantibodyrecognizesalinearantigenicepitopeofthespikeproteinofswineacutediarrhoeasyndromecoronavirus AT huizhenliu novelneutralizingmonoclonalantibodyrecognizesalinearantigenicepitopeofthespikeproteinofswineacutediarrhoeasyndromecoronavirus AT yuexiaolian novelneutralizingmonoclonalantibodyrecognizesalinearantigenicepitopeofthespikeproteinofswineacutediarrhoeasyndromecoronavirus AT yujunzhu novelneutralizingmonoclonalantibodyrecognizesalinearantigenicepitopeofthespikeproteinofswineacutediarrhoeasyndromecoronavirus AT miaoliwu novelneutralizingmonoclonalantibodyrecognizesalinearantigenicepitopeofthespikeproteinofswineacutediarrhoeasyndromecoronavirus AT jianboliu novelneutralizingmonoclonalantibodyrecognizesalinearantigenicepitopeofthespikeproteinofswineacutediarrhoeasyndromecoronavirus AT fengcong novelneutralizingmonoclonalantibodyrecognizesalinearantigenicepitopeofthespikeproteinofswineacutediarrhoeasyndromecoronavirus |