Photodynamic therapy with a novel photosensitizer inhibits DSS-induced ulcerative colitis in rats via the NF-κB signaling pathway
IntroductionUlcerative colitis (UC) is an inflammatory bowel disease characterized by inflammation and ulceration of the digestive tract.MethodsPhotodynamic therapy (PDT) with a novel photosensitizer LD4 was used to treat UC rat models to explore the therapeutic effect and mechanism of LD4-PDT on UC...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1539363/full |
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| author | Yumei Rong Yumei Rong Yumei Rong Yumei Rong Yumei Rong Minghui Zhu Minghui Zhu Minghui Zhu Minghui Zhu Nan Wang Nan Wang Nan Wang Nan Wang Feiyu Zhang Feiyu Zhang Feiyu Zhang Feiyu Zhang Tianjun Liu |
| author_facet | Yumei Rong Yumei Rong Yumei Rong Yumei Rong Yumei Rong Minghui Zhu Minghui Zhu Minghui Zhu Minghui Zhu Nan Wang Nan Wang Nan Wang Nan Wang Feiyu Zhang Feiyu Zhang Feiyu Zhang Feiyu Zhang Tianjun Liu |
| author_sort | Yumei Rong |
| collection | DOAJ |
| description | IntroductionUlcerative colitis (UC) is an inflammatory bowel disease characterized by inflammation and ulceration of the digestive tract.MethodsPhotodynamic therapy (PDT) with a novel photosensitizer LD4 was used to treat UC rat models to explore the therapeutic effect and mechanism of LD4-PDT on UC. 16S ribosomal RNA was used to detect the composition of Gut microbiota.ResultsOur findings indicate that LD4-PDT could protect the integrity of the colonic mucosa, alleviate the inflammatory response and promote the healing of colonic mucosa. Mechanism studies demonstrated that LD4-PDT could inhibit the NF-κB signaling pathway, downregulated the expression of the inflammatory factors’ tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and myeloperoxidase (MPO), increased the contents of glutathione (GSH) and superoxide dismutase (SOD) and decreased the content of malondialdehyde (MDA). Additionally, analysis of gut microbiota revealed that LD4-PDT treatment could decrease the abundance of the Proteobacteria phylum in fecal samples, while no significant differences were observed in the Firmicutes, Bacteroidetes, or Actinobacteria phyla among the three groups using 16S rRNA analysis.DiscussionIn summary, our data suggested that LD4-PDT could inhibit DSS-induced UC in rats via the NF-κB signaling pathway, indicating its potential as a novel photosensitizer for the treatment of UC. |
| format | Article |
| id | doaj-art-4e1302ad3442445a8f03a116fcb89ef9 |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-4e1302ad3442445a8f03a116fcb89ef92025-01-08T06:11:43ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-01-011510.3389/fphar.2024.15393631539363Photodynamic therapy with a novel photosensitizer inhibits DSS-induced ulcerative colitis in rats via the NF-κB signaling pathwayYumei Rong0Yumei Rong1Yumei Rong2Yumei Rong3Yumei Rong4Minghui Zhu5Minghui Zhu6Minghui Zhu7Minghui Zhu8Nan Wang9Nan Wang10Nan Wang11Nan Wang12Feiyu Zhang13Feiyu Zhang14Feiyu Zhang15Feiyu Zhang16Tianjun Liu17The Third Central Hospital of Tianjin, Tianjin, ChinaTianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases, Tianjin, ChinaArtificial Cell Engineering Technology Research Center, Tianjin, ChinaTianjin Institute of Hepatobiliary Disease, Tianjin, ChinaTianjin Key Laboratory of Biomedical Material, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, ChinaThe Third Central Hospital of Tianjin, Tianjin, ChinaTianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases, Tianjin, ChinaArtificial Cell Engineering Technology Research Center, Tianjin, ChinaTianjin Institute of Hepatobiliary Disease, Tianjin, ChinaThe Third Central Hospital of Tianjin, Tianjin, ChinaTianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases, Tianjin, ChinaArtificial Cell Engineering Technology Research Center, Tianjin, ChinaTianjin Institute of Hepatobiliary Disease, Tianjin, ChinaThe Third Central Hospital of Tianjin, Tianjin, ChinaTianjin Key Laboratory of Extracorporeal Life Support for Critical Diseases, Tianjin, ChinaArtificial Cell Engineering Technology Research Center, Tianjin, ChinaTianjin Institute of Hepatobiliary Disease, Tianjin, ChinaTianjin Key Laboratory of Biomedical Material, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, ChinaIntroductionUlcerative colitis (UC) is an inflammatory bowel disease characterized by inflammation and ulceration of the digestive tract.MethodsPhotodynamic therapy (PDT) with a novel photosensitizer LD4 was used to treat UC rat models to explore the therapeutic effect and mechanism of LD4-PDT on UC. 16S ribosomal RNA was used to detect the composition of Gut microbiota.ResultsOur findings indicate that LD4-PDT could protect the integrity of the colonic mucosa, alleviate the inflammatory response and promote the healing of colonic mucosa. Mechanism studies demonstrated that LD4-PDT could inhibit the NF-κB signaling pathway, downregulated the expression of the inflammatory factors’ tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and myeloperoxidase (MPO), increased the contents of glutathione (GSH) and superoxide dismutase (SOD) and decreased the content of malondialdehyde (MDA). Additionally, analysis of gut microbiota revealed that LD4-PDT treatment could decrease the abundance of the Proteobacteria phylum in fecal samples, while no significant differences were observed in the Firmicutes, Bacteroidetes, or Actinobacteria phyla among the three groups using 16S rRNA analysis.DiscussionIn summary, our data suggested that LD4-PDT could inhibit DSS-induced UC in rats via the NF-κB signaling pathway, indicating its potential as a novel photosensitizer for the treatment of UC.https://www.frontiersin.org/articles/10.3389/fphar.2024.1539363/fullulcerative colitisDSSoxidative stressNF-κB signaling pathwaygut microbiota |
| spellingShingle | Yumei Rong Yumei Rong Yumei Rong Yumei Rong Yumei Rong Minghui Zhu Minghui Zhu Minghui Zhu Minghui Zhu Nan Wang Nan Wang Nan Wang Nan Wang Feiyu Zhang Feiyu Zhang Feiyu Zhang Feiyu Zhang Tianjun Liu Photodynamic therapy with a novel photosensitizer inhibits DSS-induced ulcerative colitis in rats via the NF-κB signaling pathway Frontiers in Pharmacology ulcerative colitis DSS oxidative stress NF-κB signaling pathway gut microbiota |
| title | Photodynamic therapy with a novel photosensitizer inhibits DSS-induced ulcerative colitis in rats via the NF-κB signaling pathway |
| title_full | Photodynamic therapy with a novel photosensitizer inhibits DSS-induced ulcerative colitis in rats via the NF-κB signaling pathway |
| title_fullStr | Photodynamic therapy with a novel photosensitizer inhibits DSS-induced ulcerative colitis in rats via the NF-κB signaling pathway |
| title_full_unstemmed | Photodynamic therapy with a novel photosensitizer inhibits DSS-induced ulcerative colitis in rats via the NF-κB signaling pathway |
| title_short | Photodynamic therapy with a novel photosensitizer inhibits DSS-induced ulcerative colitis in rats via the NF-κB signaling pathway |
| title_sort | photodynamic therapy with a novel photosensitizer inhibits dss induced ulcerative colitis in rats via the nf κb signaling pathway |
| topic | ulcerative colitis DSS oxidative stress NF-κB signaling pathway gut microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1539363/full |
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