Melatonin improves endometrial receptivity and embryo implantation via MT2/PI3K/LIF signaling pathway in sows
Abstract Background Increased backfat thickness of sows in early gestation is negative to reproductive performance. Endometrial receptivity is an important determinant of reproductive success, but it is unclear whether the effect of sow backfat thickness on litter size is associated with endometrial...
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BMC
2025-01-01
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| Series: | Journal of Animal Science and Biotechnology |
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| Online Access: | https://doi.org/10.1186/s40104-024-01137-x |
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| author | Xue Qin Menghao Yang Yang Yu Xiaolin Wang Yi Zheng Rui Cai Weijun Pang |
| author_facet | Xue Qin Menghao Yang Yang Yu Xiaolin Wang Yi Zheng Rui Cai Weijun Pang |
| author_sort | Xue Qin |
| collection | DOAJ |
| description | Abstract Background Increased backfat thickness of sows in early gestation is negative to reproductive performance. Endometrial receptivity is an important determinant of reproductive success, but it is unclear whether the effect of sow backfat thickness on litter size is associated with endometrial receptivity and whether melatonin treatment may have benefits. The present study seeks to answer these questions through in vitro and in vivo investigations. Results Excessive lipid deposition and lower melatonin levels in the uterus are detrimental to endometrial receptivity and embryo implantation in high backfat thickness sows. In cells treated with melatonin, the MT2/PI3K/LIF axis played a role in reducing lipid accumulation in porcine endometrial epithelium cells and improved endometrial receptivity. Furthermore, we found a reduction of lipids in the uterus after eight weeks of intraperitoneal administration of melatonin to HFD mice. Notably, melatonin treatment caused a significant reduction in the deposition of endometrial collagen, an increase in the number of glands, and repair of the pinopode structure, ultimately improving endometrial receptivity, promoting embryo implantation, and increasing the number of litter size of mice. Conclusions Collectively, the finding reveals the harmful effects of high backfat thickness sows on embryo implantation and highlight the role of melatonin and the MT2/PI3K/LIF axis in improving endometrial receptivity by enhancing metabolism and reducing the levels of uterine lipids in obese animals. |
| format | Article |
| id | doaj-art-4dbe5a6829e841ac93ae85eff509a259 |
| institution | Kabale University |
| issn | 2049-1891 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Animal Science and Biotechnology |
| spelling | doaj-art-4dbe5a6829e841ac93ae85eff509a2592025-01-05T12:42:34ZengBMCJournal of Animal Science and Biotechnology2049-18912025-01-0116112210.1186/s40104-024-01137-xMelatonin improves endometrial receptivity and embryo implantation via MT2/PI3K/LIF signaling pathway in sowsXue Qin0Menghao Yang1Yang Yu2Xiaolin Wang3Yi Zheng4Rui Cai5Weijun Pang6Key Laboratory of Northwest China’s Pig Breading and Reproduction, Ministry of Agriculture and Rural Affairs of the People’s Republic of China, College of Animal Science and Technology, Northwest A&F UniversityKey Laboratory of Northwest China’s Pig Breading and Reproduction, Ministry of Agriculture and Rural Affairs of the People’s Republic of China, College of Animal Science and Technology, Northwest A&F UniversityKey Laboratory of Northwest China’s Pig Breading and Reproduction, Ministry of Agriculture and Rural Affairs of the People’s Republic of China, College of Animal Science and Technology, Northwest A&F UniversityKey Laboratory of Northwest China’s Pig Breading and Reproduction, Ministry of Agriculture and Rural Affairs of the People’s Republic of China, College of Animal Science and Technology, Northwest A&F UniversityKey Laboratory of Northwest China’s Pig Breading and Reproduction, Ministry of Agriculture and Rural Affairs of the People’s Republic of China, College of Animal Science and Technology, Northwest A&F UniversityKey Laboratory of Northwest China’s Pig Breading and Reproduction, Ministry of Agriculture and Rural Affairs of the People’s Republic of China, College of Animal Science and Technology, Northwest A&F UniversityKey Laboratory of Northwest China’s Pig Breading and Reproduction, Ministry of Agriculture and Rural Affairs of the People’s Republic of China, College of Animal Science and Technology, Northwest A&F UniversityAbstract Background Increased backfat thickness of sows in early gestation is negative to reproductive performance. Endometrial receptivity is an important determinant of reproductive success, but it is unclear whether the effect of sow backfat thickness on litter size is associated with endometrial receptivity and whether melatonin treatment may have benefits. The present study seeks to answer these questions through in vitro and in vivo investigations. Results Excessive lipid deposition and lower melatonin levels in the uterus are detrimental to endometrial receptivity and embryo implantation in high backfat thickness sows. In cells treated with melatonin, the MT2/PI3K/LIF axis played a role in reducing lipid accumulation in porcine endometrial epithelium cells and improved endometrial receptivity. Furthermore, we found a reduction of lipids in the uterus after eight weeks of intraperitoneal administration of melatonin to HFD mice. Notably, melatonin treatment caused a significant reduction in the deposition of endometrial collagen, an increase in the number of glands, and repair of the pinopode structure, ultimately improving endometrial receptivity, promoting embryo implantation, and increasing the number of litter size of mice. Conclusions Collectively, the finding reveals the harmful effects of high backfat thickness sows on embryo implantation and highlight the role of melatonin and the MT2/PI3K/LIF axis in improving endometrial receptivity by enhancing metabolism and reducing the levels of uterine lipids in obese animals.https://doi.org/10.1186/s40104-024-01137-xBackfat thicknessEmbryo implantationEndometrial receptivityMelatoninMT2/PI3K/LIFSow |
| spellingShingle | Xue Qin Menghao Yang Yang Yu Xiaolin Wang Yi Zheng Rui Cai Weijun Pang Melatonin improves endometrial receptivity and embryo implantation via MT2/PI3K/LIF signaling pathway in sows Journal of Animal Science and Biotechnology Backfat thickness Embryo implantation Endometrial receptivity Melatonin MT2/PI3K/LIF Sow |
| title | Melatonin improves endometrial receptivity and embryo implantation via MT2/PI3K/LIF signaling pathway in sows |
| title_full | Melatonin improves endometrial receptivity and embryo implantation via MT2/PI3K/LIF signaling pathway in sows |
| title_fullStr | Melatonin improves endometrial receptivity and embryo implantation via MT2/PI3K/LIF signaling pathway in sows |
| title_full_unstemmed | Melatonin improves endometrial receptivity and embryo implantation via MT2/PI3K/LIF signaling pathway in sows |
| title_short | Melatonin improves endometrial receptivity and embryo implantation via MT2/PI3K/LIF signaling pathway in sows |
| title_sort | melatonin improves endometrial receptivity and embryo implantation via mt2 pi3k lif signaling pathway in sows |
| topic | Backfat thickness Embryo implantation Endometrial receptivity Melatonin MT2/PI3K/LIF Sow |
| url | https://doi.org/10.1186/s40104-024-01137-x |
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