Gelatinolytic activity in dentin upon adhesive treatment
Abstract In this multi-parameter study, the effect of diverse factors related to adhesive application on the activation of host-derived gelatinases was investigated by gelatin zymography, in-situ zymography, fluorogenic DQ-gelatin assay and micro-tensile bond-strength (μTBS) testing. Gelatin zymogra...
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Nature Portfolio
2024-11-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-78042-w |
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| author | Xin Li Jennifer Vandooren Mariano Simón Pedano Jan De Munck Jorge Perdigão Kirsten Van Landuyt Bart Van Meerbeek |
| author_facet | Xin Li Jennifer Vandooren Mariano Simón Pedano Jan De Munck Jorge Perdigão Kirsten Van Landuyt Bart Van Meerbeek |
| author_sort | Xin Li |
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| description | Abstract In this multi-parameter study, the effect of diverse factors related to adhesive application on the activation of host-derived gelatinases was investigated by gelatin zymography, in-situ zymography, fluorogenic DQ-gelatin assay and micro-tensile bond-strength (μTBS) testing. Gelatin zymography disclosed the presence of gelatinases in phosphoric acid-etched dentin powder, while two gold-standard adhesives generated no measurable MMP activation. In-situ zymography revealed that the interfacial gelatinolytic activity from specimens treated with the two adhesives appeared similar as that of the EDTA negative control, indicating no detectable gelatinases were activated upon adhesive treatment. In solution, MMP-2/9 activity significantly decreased upon interaction with both adhesives (two-way linear mixed effects model [LMEM]: p < 0.05); gelatinases were almost completely deactivated upon 1-week incubation at 37 °C (general linear model: p < 0.05); light-curing adhesives increased temperature up to 55 °C, which appeared sufficient to dramatically decrease MMP-2/9 activity (two-way ANOVA: p < 0.05). Finally, challenging adhesive-dentin interfaces with highly concentrated MMP-9 (at a much higher concentration than present in saliva) for 1 m did not significantly affect μTBS (two-way LMEM: p > 0.05). Taken together, the two adhesives did not activate but rather inhibited the release and activation of dentinal gelatinases. |
| format | Article |
| id | doaj-art-4db92ecfccfd44dcbb582e9d4f70c6d4 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-4db92ecfccfd44dcbb582e9d4f70c6d42024-11-10T12:22:36ZengNature PortfolioScientific Reports2045-23222024-11-0114111310.1038/s41598-024-78042-wGelatinolytic activity in dentin upon adhesive treatmentXin Li0Jennifer Vandooren1Mariano Simón Pedano2Jan De Munck3Jorge Perdigão4Kirsten Van Landuyt5Bart Van Meerbeek6Department of Oral Health Sciences, BIOMAT & UZ Leuven, Dentistry, KU LeuvenLaboratory of Immunobiology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, KU LeuvenDepartment of Oral Health Sciences, BIOMAT & UZ Leuven, Dentistry, KU LeuvenDepartment of Oral Health Sciences, BIOMAT & UZ Leuven, Dentistry, KU LeuvenDepartment of Restorative Sciences, University of MinnesotaDepartment of Oral Health Sciences, BIOMAT & UZ Leuven, Dentistry, KU LeuvenDepartment of Oral Health Sciences, BIOMAT & UZ Leuven, Dentistry, KU LeuvenAbstract In this multi-parameter study, the effect of diverse factors related to adhesive application on the activation of host-derived gelatinases was investigated by gelatin zymography, in-situ zymography, fluorogenic DQ-gelatin assay and micro-tensile bond-strength (μTBS) testing. Gelatin zymography disclosed the presence of gelatinases in phosphoric acid-etched dentin powder, while two gold-standard adhesives generated no measurable MMP activation. In-situ zymography revealed that the interfacial gelatinolytic activity from specimens treated with the two adhesives appeared similar as that of the EDTA negative control, indicating no detectable gelatinases were activated upon adhesive treatment. In solution, MMP-2/9 activity significantly decreased upon interaction with both adhesives (two-way linear mixed effects model [LMEM]: p < 0.05); gelatinases were almost completely deactivated upon 1-week incubation at 37 °C (general linear model: p < 0.05); light-curing adhesives increased temperature up to 55 °C, which appeared sufficient to dramatically decrease MMP-2/9 activity (two-way ANOVA: p < 0.05). Finally, challenging adhesive-dentin interfaces with highly concentrated MMP-9 (at a much higher concentration than present in saliva) for 1 m did not significantly affect μTBS (two-way LMEM: p > 0.05). Taken together, the two adhesives did not activate but rather inhibited the release and activation of dentinal gelatinases.https://doi.org/10.1038/s41598-024-78042-wDentin-bonding agentsMatrix metalloproteinase 2Matrix metalloproteinase 9GelatinasesDental bondingBiodegradation |
| spellingShingle | Xin Li Jennifer Vandooren Mariano Simón Pedano Jan De Munck Jorge Perdigão Kirsten Van Landuyt Bart Van Meerbeek Gelatinolytic activity in dentin upon adhesive treatment Scientific Reports Dentin-bonding agents Matrix metalloproteinase 2 Matrix metalloproteinase 9 Gelatinases Dental bonding Biodegradation |
| title | Gelatinolytic activity in dentin upon adhesive treatment |
| title_full | Gelatinolytic activity in dentin upon adhesive treatment |
| title_fullStr | Gelatinolytic activity in dentin upon adhesive treatment |
| title_full_unstemmed | Gelatinolytic activity in dentin upon adhesive treatment |
| title_short | Gelatinolytic activity in dentin upon adhesive treatment |
| title_sort | gelatinolytic activity in dentin upon adhesive treatment |
| topic | Dentin-bonding agents Matrix metalloproteinase 2 Matrix metalloproteinase 9 Gelatinases Dental bonding Biodegradation |
| url | https://doi.org/10.1038/s41598-024-78042-w |
| work_keys_str_mv | AT xinli gelatinolyticactivityindentinuponadhesivetreatment AT jennifervandooren gelatinolyticactivityindentinuponadhesivetreatment AT marianosimonpedano gelatinolyticactivityindentinuponadhesivetreatment AT jandemunck gelatinolyticactivityindentinuponadhesivetreatment AT jorgeperdigao gelatinolyticactivityindentinuponadhesivetreatment AT kirstenvanlanduyt gelatinolyticactivityindentinuponadhesivetreatment AT bartvanmeerbeek gelatinolyticactivityindentinuponadhesivetreatment |