Integron-Mediated Antimicrobial Resistance and Virulence Factors in <i>Salmonella</i> Typhimurium Isolated from Poultry
This study investigates antimicrobial-resistant (AMR) <i>Salmonella</i> Typhimurium in poultry, focusing on how class I integrons contribute to AMR and virulence. Using whole genome sequencing, researchers analyzed 26 <i>S.</i> Typhimurium isolates from U.S. poultry, finding...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/14/23/3483 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846124571961327616 |
|---|---|
| author | Elizabeth Kim Nora Jean Nealon Katherine A. Murray Cydney Jardine Roberta Magnuson Sangeeta Rao |
| author_facet | Elizabeth Kim Nora Jean Nealon Katherine A. Murray Cydney Jardine Roberta Magnuson Sangeeta Rao |
| author_sort | Elizabeth Kim |
| collection | DOAJ |
| description | This study investigates antimicrobial-resistant (AMR) <i>Salmonella</i> Typhimurium in poultry, focusing on how class I integrons contribute to AMR and virulence. Using whole genome sequencing, researchers analyzed 26 <i>S.</i> Typhimurium isolates from U.S. poultry, finding that three isolates contained integrons (1000 base pairs each). These integron-positive isolates exhibited significantly higher resistance to beta-lactams, phenicols, and tetracyclines compared to integron-free isolates (<i>p</i> = 0.004, 0.009, and 0.02, respectively) and harbored genes like <i>ges</i>, <i>imp</i>, and <i>oxa</i>, which are linked to extended-spectrum beta-lactamase resistance. Most AMR gene classes (64%) were chromosome-based, with integron-positive isolates showing a broader array of resistance genes, including <i>catB</i> and <i>tetA</i>. Integron-bearing isolates had higher occurrences of bacteriocin genes and specific AMR genes like aminoglycoside and beta-lactam resistance genes, while integron-free isolates had more fimbrial and pilus genes. The presence of integrons may trend with increased AMR genes and virulence factors, highlighting the role of integron screening in enhancing AMR surveillance and reducing the need for high-priority antimicrobial treatments in poultry. These findings could support better AMR stewardship practices in poultry production, potentially lowering infection risks in humans and livestock. |
| format | Article |
| id | doaj-art-4da2186ca173449b9644d0ed2d7194e5 |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj-art-4da2186ca173449b9644d0ed2d7194e52024-12-13T16:21:23ZengMDPI AGAnimals2076-26152024-12-011423348310.3390/ani14233483Integron-Mediated Antimicrobial Resistance and Virulence Factors in <i>Salmonella</i> Typhimurium Isolated from PoultryElizabeth Kim0Nora Jean Nealon1Katherine A. Murray2Cydney Jardine3Roberta Magnuson4Sangeeta Rao5Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USADepartment of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USADepartment of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USADepartment of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USADepartment of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USADepartment of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins, CO 80523, USAThis study investigates antimicrobial-resistant (AMR) <i>Salmonella</i> Typhimurium in poultry, focusing on how class I integrons contribute to AMR and virulence. Using whole genome sequencing, researchers analyzed 26 <i>S.</i> Typhimurium isolates from U.S. poultry, finding that three isolates contained integrons (1000 base pairs each). These integron-positive isolates exhibited significantly higher resistance to beta-lactams, phenicols, and tetracyclines compared to integron-free isolates (<i>p</i> = 0.004, 0.009, and 0.02, respectively) and harbored genes like <i>ges</i>, <i>imp</i>, and <i>oxa</i>, which are linked to extended-spectrum beta-lactamase resistance. Most AMR gene classes (64%) were chromosome-based, with integron-positive isolates showing a broader array of resistance genes, including <i>catB</i> and <i>tetA</i>. Integron-bearing isolates had higher occurrences of bacteriocin genes and specific AMR genes like aminoglycoside and beta-lactam resistance genes, while integron-free isolates had more fimbrial and pilus genes. The presence of integrons may trend with increased AMR genes and virulence factors, highlighting the role of integron screening in enhancing AMR surveillance and reducing the need for high-priority antimicrobial treatments in poultry. These findings could support better AMR stewardship practices in poultry production, potentially lowering infection risks in humans and livestock.https://www.mdpi.com/2076-2615/14/23/3483poultryintegronsvirulence factors<i>Salmonella</i> Typhimuriumantimicrobial resistance |
| spellingShingle | Elizabeth Kim Nora Jean Nealon Katherine A. Murray Cydney Jardine Roberta Magnuson Sangeeta Rao Integron-Mediated Antimicrobial Resistance and Virulence Factors in <i>Salmonella</i> Typhimurium Isolated from Poultry Animals poultry integrons virulence factors <i>Salmonella</i> Typhimurium antimicrobial resistance |
| title | Integron-Mediated Antimicrobial Resistance and Virulence Factors in <i>Salmonella</i> Typhimurium Isolated from Poultry |
| title_full | Integron-Mediated Antimicrobial Resistance and Virulence Factors in <i>Salmonella</i> Typhimurium Isolated from Poultry |
| title_fullStr | Integron-Mediated Antimicrobial Resistance and Virulence Factors in <i>Salmonella</i> Typhimurium Isolated from Poultry |
| title_full_unstemmed | Integron-Mediated Antimicrobial Resistance and Virulence Factors in <i>Salmonella</i> Typhimurium Isolated from Poultry |
| title_short | Integron-Mediated Antimicrobial Resistance and Virulence Factors in <i>Salmonella</i> Typhimurium Isolated from Poultry |
| title_sort | integron mediated antimicrobial resistance and virulence factors in i salmonella i typhimurium isolated from poultry |
| topic | poultry integrons virulence factors <i>Salmonella</i> Typhimurium antimicrobial resistance |
| url | https://www.mdpi.com/2076-2615/14/23/3483 |
| work_keys_str_mv | AT elizabethkim integronmediatedantimicrobialresistanceandvirulencefactorsinisalmonellaityphimuriumisolatedfrompoultry AT norajeannealon integronmediatedantimicrobialresistanceandvirulencefactorsinisalmonellaityphimuriumisolatedfrompoultry AT katherineamurray integronmediatedantimicrobialresistanceandvirulencefactorsinisalmonellaityphimuriumisolatedfrompoultry AT cydneyjardine integronmediatedantimicrobialresistanceandvirulencefactorsinisalmonellaityphimuriumisolatedfrompoultry AT robertamagnuson integronmediatedantimicrobialresistanceandvirulencefactorsinisalmonellaityphimuriumisolatedfrompoultry AT sangeetarao integronmediatedantimicrobialresistanceandvirulencefactorsinisalmonellaityphimuriumisolatedfrompoultry |