Effect of nanoparticulate CaCO3 on the biological properties of calcium silicate cement
Abstract This study aimed to evaluate the effects of nanoparticulate CaCO3 (NPCC) on the biological properties of calcium silicate-based cements (CSCs), including their cytotoxicity, in vitro osteogenic activity, and interactions with rat femur tissue. The average size of NPCC was 90.3±26.0 nm. Cyto...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-84183-9 |
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| author | Quang Canh Vo Gitae Son Gyeung Mi Seon Sun Woo Um Sang Hoon Choi Hyeong-Cheol Yang |
| author_facet | Quang Canh Vo Gitae Son Gyeung Mi Seon Sun Woo Um Sang Hoon Choi Hyeong-Cheol Yang |
| author_sort | Quang Canh Vo |
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| description | Abstract This study aimed to evaluate the effects of nanoparticulate CaCO3 (NPCC) on the biological properties of calcium silicate-based cements (CSCs), including their cytotoxicity, in vitro osteogenic activity, and interactions with rat femur tissue. The average size of NPCC was 90.3±26.0 nm. Cytotoxicity and osteogenic activity assays were performed using mouse bone marrow mesenchymal stem cells (BMSCs). BMSCs exposed to the eluents from CSC alone and CSC containing 2.5% NPCC (CSC-NPCC (2.5%)) for 24 h showed decreased cell viability at an eluent concentration of 75%. In contrast, CSC-NPCCs (5%, 10%, and 20%) did not affect cell viability. Regarding osteogenic activity, CSC-NPCCs (5%, 10%, 20%) enhanced the expression of osteogenic genes, including runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), type I collagen (COL-1), and osteocalcin (OCN). Additionally, mineralization in cell cultures was enhanced by CSC-NPCC, indicating that NPCC promoted the osteogenic activity of CSCs. In rat femurs, NPCC accelerates CSC resorption and stimulates bone regeneration at the implantation site. CSC alone occupied 22.2%±3.25% of the total femoral area at the implantation site, whereas CSC-NPCC (20%) occupied only 4%. These histological findings suggest that CSC-NPCC has potential as a biodegradable bone cement for use in bone defect areas that require regeneration. |
| format | Article |
| id | doaj-art-4d54bf8e5c9f427ab76e67744737f5a9 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-4d54bf8e5c9f427ab76e67744737f5a92025-01-05T12:13:48ZengNature PortfolioScientific Reports2045-23222025-01-011511910.1038/s41598-024-84183-9Effect of nanoparticulate CaCO3 on the biological properties of calcium silicate cementQuang Canh Vo0Gitae Son1Gyeung Mi Seon2Sun Woo Um3Sang Hoon Choi4Hyeong-Cheol Yang5Department of Dental Biomaterials Science, Dental Research Institute, School of Dentistry, Seoul National UniversityDepartment of Dental Biomaterials Science, Dental Research Institute, School of Dentistry, Seoul National UniversityDepartment of Dental Biomaterials Science, Dental Research Institute, School of Dentistry, Seoul National UniversityDepartment of Dental Biomaterials Science, Dental Research Institute, School of Dentistry, Seoul National UniversityDepartment of Dental Biomaterials Science, Dental Research Institute, School of Dentistry, Seoul National UniversityDepartment of Dental Biomaterials Science, Dental Research Institute, School of Dentistry, Seoul National UniversityAbstract This study aimed to evaluate the effects of nanoparticulate CaCO3 (NPCC) on the biological properties of calcium silicate-based cements (CSCs), including their cytotoxicity, in vitro osteogenic activity, and interactions with rat femur tissue. The average size of NPCC was 90.3±26.0 nm. Cytotoxicity and osteogenic activity assays were performed using mouse bone marrow mesenchymal stem cells (BMSCs). BMSCs exposed to the eluents from CSC alone and CSC containing 2.5% NPCC (CSC-NPCC (2.5%)) for 24 h showed decreased cell viability at an eluent concentration of 75%. In contrast, CSC-NPCCs (5%, 10%, and 20%) did not affect cell viability. Regarding osteogenic activity, CSC-NPCCs (5%, 10%, 20%) enhanced the expression of osteogenic genes, including runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), type I collagen (COL-1), and osteocalcin (OCN). Additionally, mineralization in cell cultures was enhanced by CSC-NPCC, indicating that NPCC promoted the osteogenic activity of CSCs. In rat femurs, NPCC accelerates CSC resorption and stimulates bone regeneration at the implantation site. CSC alone occupied 22.2%±3.25% of the total femoral area at the implantation site, whereas CSC-NPCC (20%) occupied only 4%. These histological findings suggest that CSC-NPCC has potential as a biodegradable bone cement for use in bone defect areas that require regeneration.https://doi.org/10.1038/s41598-024-84183-9Nanoparticulate calcium carbonateCalcium silicate cementBone marrow mesenchymal stem cellsBiocompatibilityOsteogenicBone regeneration |
| spellingShingle | Quang Canh Vo Gitae Son Gyeung Mi Seon Sun Woo Um Sang Hoon Choi Hyeong-Cheol Yang Effect of nanoparticulate CaCO3 on the biological properties of calcium silicate cement Scientific Reports Nanoparticulate calcium carbonate Calcium silicate cement Bone marrow mesenchymal stem cells Biocompatibility Osteogenic Bone regeneration |
| title | Effect of nanoparticulate CaCO3 on the biological properties of calcium silicate cement |
| title_full | Effect of nanoparticulate CaCO3 on the biological properties of calcium silicate cement |
| title_fullStr | Effect of nanoparticulate CaCO3 on the biological properties of calcium silicate cement |
| title_full_unstemmed | Effect of nanoparticulate CaCO3 on the biological properties of calcium silicate cement |
| title_short | Effect of nanoparticulate CaCO3 on the biological properties of calcium silicate cement |
| title_sort | effect of nanoparticulate caco3 on the biological properties of calcium silicate cement |
| topic | Nanoparticulate calcium carbonate Calcium silicate cement Bone marrow mesenchymal stem cells Biocompatibility Osteogenic Bone regeneration |
| url | https://doi.org/10.1038/s41598-024-84183-9 |
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