Longitudinal monitoring of sewershed resistomes in socioeconomically diverse urban neighborhoods
Abstract Background Understanding factors associated with antimicrobial resistance (AMR) distribution across populations is a necessary step in planning mitigation measures. While associations between AMR and socioeconomic-status (SES), including employment and education have been increasingly recog...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Communications Medicine |
| Online Access: | https://doi.org/10.1038/s43856-024-00729-y |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841544354383003648 |
|---|---|
| author | Jangwoo Lee Kevin Xiang Emily Au Shahrzad Sarabi Nicole Acosta Srijak Bhatnagar Jennifer Van Doorn Stefania Bertazzon John M. Conly Elissa Rennert-May Johann D. D. Pitout Bonita E. Lee Xiaoli Pang Christine O’Grady Kevin Frankowski Casey R. J. Hubert Michael D. Parkins |
| author_facet | Jangwoo Lee Kevin Xiang Emily Au Shahrzad Sarabi Nicole Acosta Srijak Bhatnagar Jennifer Van Doorn Stefania Bertazzon John M. Conly Elissa Rennert-May Johann D. D. Pitout Bonita E. Lee Xiaoli Pang Christine O’Grady Kevin Frankowski Casey R. J. Hubert Michael D. Parkins |
| author_sort | Jangwoo Lee |
| collection | DOAJ |
| description | Abstract Background Understanding factors associated with antimicrobial resistance (AMR) distribution across populations is a necessary step in planning mitigation measures. While associations between AMR and socioeconomic-status (SES), including employment and education have been increasingly recognized in low- and middle-income settings, connections are less clear in high-income countries where SES remains an important influence on other health outcomes. Methods We explored the relationship between SES and AMR in Calgary, Canada using spatially-resolved wastewater-based surveillance of resistomes detected by metagenomics across eight socio-economically diverse urban neighborhoods. Resistomes were established by shotgun-sequencing of wastewater pellets, and qPCR of targeted-AMR genes. SES status was established using 2021 Canadian census data. Conducting this comparison during the height of COVID-related international travel restrictions (Dec. 2020–Oct. 2021) allowed the hypotheses linking SES and AMR to be assessed with limited confounding. These were compared with sewage metagenomes from 244 cities around the world, linked with Human Development Index (HDI). Results Wastewater metagenomes from Calgary’s socioeconomically diverse neighborhoods exhibit highly similar resistomes, with no quantitative differences (p > 0.05), low Bray-Curtis dissimilarity, and no significant correlations with SES. By comparison, dissimilarity is observed between globally-sourced resistomes (p < 0.05), underscoring the homogeneity of resistomes in Calgary’s sub-populations. The analysis of globally-sourced resistomes alongside Calgary’s resistome further reveals lower AMR burden in Calgary relative to other cities around the world. This is particularly pronounced for the most clinically-relevant AMR genes (e.g., beta-lactamases, macrolide-lincosamide-streptogramin). Conclusions This work showcases the effectiveness of inclusive and comprehensive wastewater-based surveillance for exploring the interplay between SES and AMR. |
| format | Article |
| id | doaj-art-4d515c911e434dc49eb21a6ff833a717 |
| institution | Kabale University |
| issn | 2730-664X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Medicine |
| spelling | doaj-art-4d515c911e434dc49eb21a6ff833a7172025-01-12T12:37:14ZengNature PortfolioCommunications Medicine2730-664X2025-01-015111310.1038/s43856-024-00729-yLongitudinal monitoring of sewershed resistomes in socioeconomically diverse urban neighborhoodsJangwoo Lee0Kevin Xiang1Emily Au2Shahrzad Sarabi3Nicole Acosta4Srijak Bhatnagar5Jennifer Van Doorn6Stefania Bertazzon7John M. Conly8Elissa Rennert-May9Johann D. D. Pitout10Bonita E. Lee11Xiaoli Pang12Christine O’Grady13Kevin Frankowski14Casey R. J. Hubert15Michael D. Parkins16Department of Microbiology, Immunology and Infectious Diseases, University of CalgaryDepartment of Microbiology, Immunology and Infectious Diseases, University of CalgaryDepartment of Microbiology, Immunology and Infectious Diseases, University of CalgaryDepartment of Geography, University of CalgaryDepartment of Microbiology, Immunology and Infectious Diseases, University of CalgaryDepartment of Biological Sciences, University of CalgaryDepartment of Biological Sciences, University of CalgaryDepartment of Geography, University of CalgaryDepartment of Microbiology, Immunology and Infectious Diseases, University of CalgaryDepartment of Microbiology, Immunology and Infectious Diseases, University of CalgaryDepartment of Microbiology, Immunology and Infectious Diseases, University of CalgaryDepartment of Pediatrics, University of AlbertaDepartment of Laboratory Medicine and Pathology, University of AlbertaAdvancing Canadian Water Assets, University of CalgaryAdvancing Canadian Water Assets, University of CalgaryDepartment of Biological Sciences, University of CalgaryDepartment of Microbiology, Immunology and Infectious Diseases, University of CalgaryAbstract Background Understanding factors associated with antimicrobial resistance (AMR) distribution across populations is a necessary step in planning mitigation measures. While associations between AMR and socioeconomic-status (SES), including employment and education have been increasingly recognized in low- and middle-income settings, connections are less clear in high-income countries where SES remains an important influence on other health outcomes. Methods We explored the relationship between SES and AMR in Calgary, Canada using spatially-resolved wastewater-based surveillance of resistomes detected by metagenomics across eight socio-economically diverse urban neighborhoods. Resistomes were established by shotgun-sequencing of wastewater pellets, and qPCR of targeted-AMR genes. SES status was established using 2021 Canadian census data. Conducting this comparison during the height of COVID-related international travel restrictions (Dec. 2020–Oct. 2021) allowed the hypotheses linking SES and AMR to be assessed with limited confounding. These were compared with sewage metagenomes from 244 cities around the world, linked with Human Development Index (HDI). Results Wastewater metagenomes from Calgary’s socioeconomically diverse neighborhoods exhibit highly similar resistomes, with no quantitative differences (p > 0.05), low Bray-Curtis dissimilarity, and no significant correlations with SES. By comparison, dissimilarity is observed between globally-sourced resistomes (p < 0.05), underscoring the homogeneity of resistomes in Calgary’s sub-populations. The analysis of globally-sourced resistomes alongside Calgary’s resistome further reveals lower AMR burden in Calgary relative to other cities around the world. This is particularly pronounced for the most clinically-relevant AMR genes (e.g., beta-lactamases, macrolide-lincosamide-streptogramin). Conclusions This work showcases the effectiveness of inclusive and comprehensive wastewater-based surveillance for exploring the interplay between SES and AMR.https://doi.org/10.1038/s43856-024-00729-y |
| spellingShingle | Jangwoo Lee Kevin Xiang Emily Au Shahrzad Sarabi Nicole Acosta Srijak Bhatnagar Jennifer Van Doorn Stefania Bertazzon John M. Conly Elissa Rennert-May Johann D. D. Pitout Bonita E. Lee Xiaoli Pang Christine O’Grady Kevin Frankowski Casey R. J. Hubert Michael D. Parkins Longitudinal monitoring of sewershed resistomes in socioeconomically diverse urban neighborhoods Communications Medicine |
| title | Longitudinal monitoring of sewershed resistomes in socioeconomically diverse urban neighborhoods |
| title_full | Longitudinal monitoring of sewershed resistomes in socioeconomically diverse urban neighborhoods |
| title_fullStr | Longitudinal monitoring of sewershed resistomes in socioeconomically diverse urban neighborhoods |
| title_full_unstemmed | Longitudinal monitoring of sewershed resistomes in socioeconomically diverse urban neighborhoods |
| title_short | Longitudinal monitoring of sewershed resistomes in socioeconomically diverse urban neighborhoods |
| title_sort | longitudinal monitoring of sewershed resistomes in socioeconomically diverse urban neighborhoods |
| url | https://doi.org/10.1038/s43856-024-00729-y |
| work_keys_str_mv | AT jangwoolee longitudinalmonitoringofsewershedresistomesinsocioeconomicallydiverseurbanneighborhoods AT kevinxiang longitudinalmonitoringofsewershedresistomesinsocioeconomicallydiverseurbanneighborhoods AT emilyau longitudinalmonitoringofsewershedresistomesinsocioeconomicallydiverseurbanneighborhoods AT shahrzadsarabi longitudinalmonitoringofsewershedresistomesinsocioeconomicallydiverseurbanneighborhoods AT nicoleacosta longitudinalmonitoringofsewershedresistomesinsocioeconomicallydiverseurbanneighborhoods AT srijakbhatnagar longitudinalmonitoringofsewershedresistomesinsocioeconomicallydiverseurbanneighborhoods AT jennifervandoorn longitudinalmonitoringofsewershedresistomesinsocioeconomicallydiverseurbanneighborhoods AT stefaniabertazzon longitudinalmonitoringofsewershedresistomesinsocioeconomicallydiverseurbanneighborhoods AT johnmconly longitudinalmonitoringofsewershedresistomesinsocioeconomicallydiverseurbanneighborhoods AT elissarennertmay longitudinalmonitoringofsewershedresistomesinsocioeconomicallydiverseurbanneighborhoods AT johannddpitout longitudinalmonitoringofsewershedresistomesinsocioeconomicallydiverseurbanneighborhoods AT bonitaelee longitudinalmonitoringofsewershedresistomesinsocioeconomicallydiverseurbanneighborhoods AT xiaolipang longitudinalmonitoringofsewershedresistomesinsocioeconomicallydiverseurbanneighborhoods AT christineogrady longitudinalmonitoringofsewershedresistomesinsocioeconomicallydiverseurbanneighborhoods AT kevinfrankowski longitudinalmonitoringofsewershedresistomesinsocioeconomicallydiverseurbanneighborhoods AT caseyrjhubert longitudinalmonitoringofsewershedresistomesinsocioeconomicallydiverseurbanneighborhoods AT michaeldparkins longitudinalmonitoringofsewershedresistomesinsocioeconomicallydiverseurbanneighborhoods |