Biomolecule screen identifies several inhibitors of Salmonella enterica surface colonization
Salmonella enterica is a foodborne pathogen commonly found in agricultural facilities; its prevalence, as well as increasing levels of disinfectant- and antibiotic-resistance, has significant costs for agriculture as well as human health. In an effort to identify potential new inhibitors of S. enter...
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| Format: | Article |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1467511/full |
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| author | Joseph Headrick Amital Ohayon Shannon Elliott Jacob Schultz Erez Mills Erik Petersen |
| author_facet | Joseph Headrick Amital Ohayon Shannon Elliott Jacob Schultz Erez Mills Erik Petersen |
| author_sort | Joseph Headrick |
| collection | DOAJ |
| description | Salmonella enterica is a foodborne pathogen commonly found in agricultural facilities; its prevalence, as well as increasing levels of disinfectant- and antibiotic-resistance, has significant costs for agriculture as well as human health. In an effort to identify potential new inhibitors of S. enterica on abiotic surfaces, we developed a biomolecule screen of nutrient-type compounds because nutrients would have lower toxicity in animal facilities and bacterial nutrient utilization pathways might prove less susceptible to the development of bacterial resistance. After screening 285 nutrient-type compounds, we identified ten that significantly inhibited the ability of S. enterica to colonize a plastic surface. After conducting a dose-response curve, salicylic acid was selected for further testing due to its low minimal inhibitory concentration (62.5 μM) as well as a low total inhibitory concentration (250 μM). Salicylic acid was also able to inhibit surface colonization of a wide range of bacterial pathogens, suggesting that our biomolecule screen might have broader application beyond S. enterica. Finally, we determined that salicylic acid was also able to inhibit S. enterica colonization of an organic surface on eggshells. Together, these results suggest that nutrient-type biomolecules may provide an avenue for preventing resistant bacteria from contaminating surfaces. |
| format | Article |
| id | doaj-art-4d45d6f60cc64822970bae2cc8e693b1 |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-4d45d6f60cc64822970bae2cc8e693b12025-01-03T04:12:55ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-01-011210.3389/fbioe.2024.14675111467511Biomolecule screen identifies several inhibitors of Salmonella enterica surface colonizationJoseph Headrick0Amital Ohayon1Shannon Elliott2Jacob Schultz3Erez Mills4Erik Petersen5Department of Biomedical Health Sciences, College of Public Health, East Tennessee State University, Johnson City, TN, United StatesDepartment of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, Rehovot, IsraelDepartment of Biomedical Health Sciences, College of Public Health, East Tennessee State University, Johnson City, TN, United StatesDepartment of Environmental and Occupational Health and Safety Sciences, College of Public Health, East Tennessee State University, Johnson City, TN, United StatesDepartment of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food, and Environment, The Hebrew University of Jerusalem, Rehovot, IsraelDepartment of Biomedical Health Sciences, College of Public Health, East Tennessee State University, Johnson City, TN, United StatesSalmonella enterica is a foodborne pathogen commonly found in agricultural facilities; its prevalence, as well as increasing levels of disinfectant- and antibiotic-resistance, has significant costs for agriculture as well as human health. In an effort to identify potential new inhibitors of S. enterica on abiotic surfaces, we developed a biomolecule screen of nutrient-type compounds because nutrients would have lower toxicity in animal facilities and bacterial nutrient utilization pathways might prove less susceptible to the development of bacterial resistance. After screening 285 nutrient-type compounds, we identified ten that significantly inhibited the ability of S. enterica to colonize a plastic surface. After conducting a dose-response curve, salicylic acid was selected for further testing due to its low minimal inhibitory concentration (62.5 μM) as well as a low total inhibitory concentration (250 μM). Salicylic acid was also able to inhibit surface colonization of a wide range of bacterial pathogens, suggesting that our biomolecule screen might have broader application beyond S. enterica. Finally, we determined that salicylic acid was also able to inhibit S. enterica colonization of an organic surface on eggshells. Together, these results suggest that nutrient-type biomolecules may provide an avenue for preventing resistant bacteria from contaminating surfaces.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1467511/fullSalmonellasurface colonizationsalicylic acidbiomoleculeeggshell |
| spellingShingle | Joseph Headrick Amital Ohayon Shannon Elliott Jacob Schultz Erez Mills Erik Petersen Biomolecule screen identifies several inhibitors of Salmonella enterica surface colonization Frontiers in Bioengineering and Biotechnology Salmonella surface colonization salicylic acid biomolecule eggshell |
| title | Biomolecule screen identifies several inhibitors of Salmonella enterica surface colonization |
| title_full | Biomolecule screen identifies several inhibitors of Salmonella enterica surface colonization |
| title_fullStr | Biomolecule screen identifies several inhibitors of Salmonella enterica surface colonization |
| title_full_unstemmed | Biomolecule screen identifies several inhibitors of Salmonella enterica surface colonization |
| title_short | Biomolecule screen identifies several inhibitors of Salmonella enterica surface colonization |
| title_sort | biomolecule screen identifies several inhibitors of salmonella enterica surface colonization |
| topic | Salmonella surface colonization salicylic acid biomolecule eggshell |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1467511/full |
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