Effects of dietary sulfated polysaccharides extracted from brown macroalgae Sargassum ilicifolium on growth indices, antioxidant status, and immune genes expression in white shrimp (Litopenaeus vannamei)
This feeding trial aimed to evaluate the impacts of dietary sulfated polysaccharides extracted from brown macroalgae Sargassum ilicifolium on growth, antioxidant status, and immune genes expression in white shrimp, Litopenaeus vannamei. A total of 225 shrimp (3.91 ± 0.02 g) were stocked into fifteen...
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Elsevier
2024-12-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424004757 |
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| author | Maryam Pourazad Dara Bagheri Ebrahim Sotoudeh Ahmad Ghasemi |
| author_facet | Maryam Pourazad Dara Bagheri Ebrahim Sotoudeh Ahmad Ghasemi |
| author_sort | Maryam Pourazad |
| collection | DOAJ |
| description | This feeding trial aimed to evaluate the impacts of dietary sulfated polysaccharides extracted from brown macroalgae Sargassum ilicifolium on growth, antioxidant status, and immune genes expression in white shrimp, Litopenaeus vannamei. A total of 225 shrimp (3.91 ± 0.02 g) were stocked into fifteen 300-L fiberglass tanks (15 shrimp/tank) and divided into five groups fed diets supplemented with 0 (control), 0.5 (SIP0.5), 1 (SIP1), 2 (SIP2), and 4 (SIP4) g/kg diet of S. ilicifolium-extracted sulfated polysaccharides (SIP). After 8 weeks, growth and feed utilization indices and muscle composition in white shrimp were not significantly different (P > 0.05). All shrimps exhibited a high survival (above 80 %). While catalase and glutathione S-transferase (GST) activities in the hepatopancreas of shrimp were not significantly different among treatments, hepatopancreas glutathione (GSH) content of shrimp fed diet supplemented with 4 g SIP/kg diet (SIP4) was significantly higher, then SIP2 treatment, and lower in other shrimps (P < 0.05). Also, incorporating 4 g SIP/kg diet resulted in a significant lower malondialdehyde (MDA) content in the hepatopancreas of shrimp than the control (P < 0.05). Prophenoloxidase relative expression was highest in the hepatopancreas of shrimp fed diet supplemented with 2 g/kg of S. ilicifolium-extracted sulfated polysaccharides (SIP2), followed by 0.5 and 1 g/kg SIP fed treatments, then 4 g/kg SIP fed group and lowest in the control (P < 0.05). The higher relative expressions of SOD and penaeidin-3a genes in the hepatopancreas were also seen in the SIP fed shrimps. Moreover, a significant increase in hepatopancreas lysozyme expression was recorded with increasing SIP administration levels. Thus, sulfated polysaccharides extracted from S. ilicifolium at levels of 2–4 g/kg diet have antioxidant and immunostimulatory effects in white shrimp and can be safely used as an immunostimulant for juvenile L. vannamei without impairing growth performance and biochemical muscle composition. |
| format | Article |
| id | doaj-art-4d45b8489e694879a407a55a02537065 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
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| series | Aquaculture Reports |
| spelling | doaj-art-4d45b8489e694879a407a55a025370652024-12-03T04:29:04ZengElsevierAquaculture Reports2352-51342024-12-0139102387Effects of dietary sulfated polysaccharides extracted from brown macroalgae Sargassum ilicifolium on growth indices, antioxidant status, and immune genes expression in white shrimp (Litopenaeus vannamei)Maryam Pourazad0Dara Bagheri1Ebrahim Sotoudeh2Ahmad Ghasemi3Department of Fisheries, Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr 75169, IranDepartment of Fisheries, Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr 75169, Iran; Corresponding author.Department of Fisheries, Faculty of Nano and Bio Science and Technology, Persian Gulf University, Bushehr 75169, IranPersian Gulf Research Center, Persian Gulf University, Bushehr 75169, IranThis feeding trial aimed to evaluate the impacts of dietary sulfated polysaccharides extracted from brown macroalgae Sargassum ilicifolium on growth, antioxidant status, and immune genes expression in white shrimp, Litopenaeus vannamei. A total of 225 shrimp (3.91 ± 0.02 g) were stocked into fifteen 300-L fiberglass tanks (15 shrimp/tank) and divided into five groups fed diets supplemented with 0 (control), 0.5 (SIP0.5), 1 (SIP1), 2 (SIP2), and 4 (SIP4) g/kg diet of S. ilicifolium-extracted sulfated polysaccharides (SIP). After 8 weeks, growth and feed utilization indices and muscle composition in white shrimp were not significantly different (P > 0.05). All shrimps exhibited a high survival (above 80 %). While catalase and glutathione S-transferase (GST) activities in the hepatopancreas of shrimp were not significantly different among treatments, hepatopancreas glutathione (GSH) content of shrimp fed diet supplemented with 4 g SIP/kg diet (SIP4) was significantly higher, then SIP2 treatment, and lower in other shrimps (P < 0.05). Also, incorporating 4 g SIP/kg diet resulted in a significant lower malondialdehyde (MDA) content in the hepatopancreas of shrimp than the control (P < 0.05). Prophenoloxidase relative expression was highest in the hepatopancreas of shrimp fed diet supplemented with 2 g/kg of S. ilicifolium-extracted sulfated polysaccharides (SIP2), followed by 0.5 and 1 g/kg SIP fed treatments, then 4 g/kg SIP fed group and lowest in the control (P < 0.05). The higher relative expressions of SOD and penaeidin-3a genes in the hepatopancreas were also seen in the SIP fed shrimps. Moreover, a significant increase in hepatopancreas lysozyme expression was recorded with increasing SIP administration levels. Thus, sulfated polysaccharides extracted from S. ilicifolium at levels of 2–4 g/kg diet have antioxidant and immunostimulatory effects in white shrimp and can be safely used as an immunostimulant for juvenile L. vannamei without impairing growth performance and biochemical muscle composition.http://www.sciencedirect.com/science/article/pii/S2352513424004757SeaweedAlgaeSargassum ilicifoliumImmunityLitopenaeus vannamei |
| spellingShingle | Maryam Pourazad Dara Bagheri Ebrahim Sotoudeh Ahmad Ghasemi Effects of dietary sulfated polysaccharides extracted from brown macroalgae Sargassum ilicifolium on growth indices, antioxidant status, and immune genes expression in white shrimp (Litopenaeus vannamei) Aquaculture Reports Seaweed Algae Sargassum ilicifolium Immunity Litopenaeus vannamei |
| title | Effects of dietary sulfated polysaccharides extracted from brown macroalgae Sargassum ilicifolium on growth indices, antioxidant status, and immune genes expression in white shrimp (Litopenaeus vannamei) |
| title_full | Effects of dietary sulfated polysaccharides extracted from brown macroalgae Sargassum ilicifolium on growth indices, antioxidant status, and immune genes expression in white shrimp (Litopenaeus vannamei) |
| title_fullStr | Effects of dietary sulfated polysaccharides extracted from brown macroalgae Sargassum ilicifolium on growth indices, antioxidant status, and immune genes expression in white shrimp (Litopenaeus vannamei) |
| title_full_unstemmed | Effects of dietary sulfated polysaccharides extracted from brown macroalgae Sargassum ilicifolium on growth indices, antioxidant status, and immune genes expression in white shrimp (Litopenaeus vannamei) |
| title_short | Effects of dietary sulfated polysaccharides extracted from brown macroalgae Sargassum ilicifolium on growth indices, antioxidant status, and immune genes expression in white shrimp (Litopenaeus vannamei) |
| title_sort | effects of dietary sulfated polysaccharides extracted from brown macroalgae sargassum ilicifolium on growth indices antioxidant status and immune genes expression in white shrimp litopenaeus vannamei |
| topic | Seaweed Algae Sargassum ilicifolium Immunity Litopenaeus vannamei |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424004757 |
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