Transcriptome Analysis of Rainbow Trout (Oncorhynchus mykiss) Eggs Subjected to the High Hydrostatic Pressure Treatment
High hydrostatic pressure (HHP) causes depolymerization of the spindle microtubules. HHP applied to fish eggs prevents extrusion of the second polar body and inhibits the first cell cleavage, and it is used to produce triploids and diploid gynogenetic and androgenetic individuals. HHP has been also...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2018/5197126 |
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| author | Artur Gurgul Klaudia Pawlina-Tyszko Monika Bugno-Poniewierska Tomasz Szmatoła Igor Jasielczuk Stefan Dobosz Konrad Ocalewicz |
| author_facet | Artur Gurgul Klaudia Pawlina-Tyszko Monika Bugno-Poniewierska Tomasz Szmatoła Igor Jasielczuk Stefan Dobosz Konrad Ocalewicz |
| author_sort | Artur Gurgul |
| collection | DOAJ |
| description | High hydrostatic pressure (HHP) causes depolymerization of the spindle microtubules. HHP applied to fish eggs prevents extrusion of the second polar body and inhibits the first cell cleavage, and it is used to produce triploids and diploid gynogenetic and androgenetic individuals. HHP has been also found to affect biomolecules including nucleic acids, and it may be presumed that HHP administered to the rainbow trout (Oncorhynchus mykiss) eggs disturbs cytoplasmic maternal RNA indispensable for the early embryogenesis. To verify this assumption, quality and quantity of RNA extracted from the rainbow trout eggs subjected to the high hydrostatic pressure shock were analyzed. Provided results exhibited that maternal transcriptome was resistant to a three-minute exposure to 65.5 MPa of HHP treatment. Some trend showing increase of the RNA integrity was observed in the HHP-treated eggs; however, the difference was not statistically significant. Alterations in the expression profiles in the rainbow trout eggs subjected to HHP were also negligible. Greater differences in the maternal gene expression were observed between eggs from different clutches than between HHP-treated and untreated eggs from the same clutch. It may be assumed that exposure to HHP shock was too short to modify significantly maternal transcripts in the rainbow trout eggs. |
| format | Article |
| id | doaj-art-4cecb983ab3e4483a3e69a94c9c1403d |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-4cecb983ab3e4483a3e69a94c9c1403d2025-02-03T05:47:47ZengWileyInternational Journal of Genomics2314-436X2314-43782018-01-01201810.1155/2018/51971265197126Transcriptome Analysis of Rainbow Trout (Oncorhynchus mykiss) Eggs Subjected to the High Hydrostatic Pressure TreatmentArtur Gurgul0Klaudia Pawlina-Tyszko1Monika Bugno-Poniewierska2Tomasz Szmatoła3Igor Jasielczuk4Stefan Dobosz5Konrad Ocalewicz6Laboratory of Genomics, Department of Animal Genetics and Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandLaboratory of Genomics, Department of Animal Genetics and Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandInstitute of Veterinary Sciences, University of Agriculture in Krakow, Mickiewicza 24/28 Av, 30-059 Krakow, PolandLaboratory of Genomics, Department of Animal Genetics and Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandLaboratory of Genomics, Department of Animal Genetics and Molecular Biology, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, PolandDepartment of Salmonid Research, Inland Fisheries Institute in Olsztyn, Rutki, 83-330 Żukowo, PolandDepartment of Marine Biology and Ecology, Institute of Oceanography, Faculty of Oceanography and Geography, University of Gdansk, M. Piłsudskiego 46 Av, 81-378 Gdynia, PolandHigh hydrostatic pressure (HHP) causes depolymerization of the spindle microtubules. HHP applied to fish eggs prevents extrusion of the second polar body and inhibits the first cell cleavage, and it is used to produce triploids and diploid gynogenetic and androgenetic individuals. HHP has been also found to affect biomolecules including nucleic acids, and it may be presumed that HHP administered to the rainbow trout (Oncorhynchus mykiss) eggs disturbs cytoplasmic maternal RNA indispensable for the early embryogenesis. To verify this assumption, quality and quantity of RNA extracted from the rainbow trout eggs subjected to the high hydrostatic pressure shock were analyzed. Provided results exhibited that maternal transcriptome was resistant to a three-minute exposure to 65.5 MPa of HHP treatment. Some trend showing increase of the RNA integrity was observed in the HHP-treated eggs; however, the difference was not statistically significant. Alterations in the expression profiles in the rainbow trout eggs subjected to HHP were also negligible. Greater differences in the maternal gene expression were observed between eggs from different clutches than between HHP-treated and untreated eggs from the same clutch. It may be assumed that exposure to HHP shock was too short to modify significantly maternal transcripts in the rainbow trout eggs.http://dx.doi.org/10.1155/2018/5197126 |
| spellingShingle | Artur Gurgul Klaudia Pawlina-Tyszko Monika Bugno-Poniewierska Tomasz Szmatoła Igor Jasielczuk Stefan Dobosz Konrad Ocalewicz Transcriptome Analysis of Rainbow Trout (Oncorhynchus mykiss) Eggs Subjected to the High Hydrostatic Pressure Treatment International Journal of Genomics |
| title | Transcriptome Analysis of Rainbow Trout (Oncorhynchus mykiss) Eggs Subjected to the High Hydrostatic Pressure Treatment |
| title_full | Transcriptome Analysis of Rainbow Trout (Oncorhynchus mykiss) Eggs Subjected to the High Hydrostatic Pressure Treatment |
| title_fullStr | Transcriptome Analysis of Rainbow Trout (Oncorhynchus mykiss) Eggs Subjected to the High Hydrostatic Pressure Treatment |
| title_full_unstemmed | Transcriptome Analysis of Rainbow Trout (Oncorhynchus mykiss) Eggs Subjected to the High Hydrostatic Pressure Treatment |
| title_short | Transcriptome Analysis of Rainbow Trout (Oncorhynchus mykiss) Eggs Subjected to the High Hydrostatic Pressure Treatment |
| title_sort | transcriptome analysis of rainbow trout oncorhynchus mykiss eggs subjected to the high hydrostatic pressure treatment |
| url | http://dx.doi.org/10.1155/2018/5197126 |
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