Anticonvulsant, Anticholinesterase and Cytoprotective Effects of the Aqueous Extract of <i>Lippia sidoides</i> Cham
(1) Background: <i>Lippia sidoides</i> Cham is a Brazilian aromatic plant rich in phenolic compounds. In traditional medicine, its leaves are used to treat diseases of the Central Nervous System such as stress and anxiety. This study evaluates the capacity of the aqueous extract of <i...
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2024-10-01
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| author | Cicera Janaine Camilo Débora Odília Duarte Leite Natália Kelly Gomes de Carvalho José Walber Gonçalves Castro Jane Eire Silva Alencar de Menezes Helcio Silva dos Santos Joanda Paolla Raimundo Silva Daniela Ribeiro Alves Selene Maia de Morais Fabíola Fernandes Galvão Rodrigues José Galberto Martins da Costa |
| author_facet | Cicera Janaine Camilo Débora Odília Duarte Leite Natália Kelly Gomes de Carvalho José Walber Gonçalves Castro Jane Eire Silva Alencar de Menezes Helcio Silva dos Santos Joanda Paolla Raimundo Silva Daniela Ribeiro Alves Selene Maia de Morais Fabíola Fernandes Galvão Rodrigues José Galberto Martins da Costa |
| author_sort | Cicera Janaine Camilo |
| collection | DOAJ |
| description | (1) Background: <i>Lippia sidoides</i> Cham is a Brazilian aromatic plant rich in phenolic compounds. In traditional medicine, its leaves are used to treat diseases of the Central Nervous System such as stress and anxiety. This study evaluates the capacity of the aqueous extract of <i>L. sidoides</i> as an anticonvulsant, anticholinesterase and antihemolytic agent. (2) Methods: The extract was obtained from the leaves using water as a solvent, then dried in a spray dryer. The anticonvulsant effect was evaluated in zebrafish models using the pentylenetetrazol (PTZ) method. The anticholinesterase effect was determined using the acetylcholinesterase enzyme and physostigmine as a positive control. The antihemolytic action was evaluated by exposing erythrocytes to different concentrations of NaCl in the presence and absence of the extract. (3) Results: The anticonvulsant effect was observed at a concentration of 400 mg/kg, delaying convulsive crises. In the anticholinesterase assay, a dose-dependent action and variation in the effect over time were observed, demonstrating a reversible effect of the extract. For the osmotic fragility test, the extract showed satisfactory results, providing cellular protection across all variations of NaCl concentration. (4) Conclusions: These results demonstrate the promising potential of <i>L. sidoides</i> extract for the development of drugs that act in the treatment of diseases that affect the Central Nervous System. |
| format | Article |
| id | doaj-art-4cd82479691e459f990ef3a41a7b21c2 |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-4cd82479691e459f990ef3a41a7b21c22024-11-08T14:39:26ZengMDPI AGPlants2223-77472024-10-011321303410.3390/plants13213034Anticonvulsant, Anticholinesterase and Cytoprotective Effects of the Aqueous Extract of <i>Lippia sidoides</i> ChamCicera Janaine Camilo0Débora Odília Duarte Leite1Natália Kelly Gomes de Carvalho2José Walber Gonçalves Castro3Jane Eire Silva Alencar de Menezes4Helcio Silva dos Santos5Joanda Paolla Raimundo Silva6Daniela Ribeiro Alves7Selene Maia de Morais8Fabíola Fernandes Galvão Rodrigues9José Galberto Martins da Costa10Federal University of Campina Grande, Campina Grande 58429-900, Paraíba, BrazilPostgraduate Program in Biological Chemistry, Department of Biological Chemistry, Regional University of Cariri, Crato 63105-00, Ceará, BrazilPostgraduate Program in Biological Chemistry, Department of Biological Chemistry, Regional University of Cariri, Crato 63105-00, Ceará, BrazilPostgraduate Program in Biological Chemistry, Department of Biological Chemistry, Regional University of Cariri, Crato 63105-00, Ceará, BrazilPostgraduate Program in Natural Sciences-PPGCN, State University of Ceara, Fortaleza 60714-903, Ceará, BrazilPostgraduate Program in Biological Chemistry, Department of Biological Chemistry, Regional University of Cariri, Crato 63105-00, Ceará, BrazilMultiuser Laboratory of Characterization and Analysis, Federal University of Paraíba, João Pessoa 58051-900, Paraíba, BrazilAnimal Health Research Center—NUPESA, Ceara State University, Av. Dr. Silas Munguba—1700, Campus Itaperi, Fortaleza 60714-903, Ceará, BrazilAnimal Health Research Center—NUPESA, Ceara State University, Av. Dr. Silas Munguba—1700, Campus Itaperi, Fortaleza 60714-903, Ceará, BrazilPostgraduate Program in Biological Chemistry, Department of Biological Chemistry, Regional University of Cariri, Crato 63105-00, Ceará, BrazilPostgraduate Program in Biological Chemistry, Department of Biological Chemistry, Regional University of Cariri, Crato 63105-00, Ceará, Brazil(1) Background: <i>Lippia sidoides</i> Cham is a Brazilian aromatic plant rich in phenolic compounds. In traditional medicine, its leaves are used to treat diseases of the Central Nervous System such as stress and anxiety. This study evaluates the capacity of the aqueous extract of <i>L. sidoides</i> as an anticonvulsant, anticholinesterase and antihemolytic agent. (2) Methods: The extract was obtained from the leaves using water as a solvent, then dried in a spray dryer. The anticonvulsant effect was evaluated in zebrafish models using the pentylenetetrazol (PTZ) method. The anticholinesterase effect was determined using the acetylcholinesterase enzyme and physostigmine as a positive control. The antihemolytic action was evaluated by exposing erythrocytes to different concentrations of NaCl in the presence and absence of the extract. (3) Results: The anticonvulsant effect was observed at a concentration of 400 mg/kg, delaying convulsive crises. In the anticholinesterase assay, a dose-dependent action and variation in the effect over time were observed, demonstrating a reversible effect of the extract. For the osmotic fragility test, the extract showed satisfactory results, providing cellular protection across all variations of NaCl concentration. (4) Conclusions: These results demonstrate the promising potential of <i>L. sidoides</i> extract for the development of drugs that act in the treatment of diseases that affect the Central Nervous System.https://www.mdpi.com/2223-7747/13/21/3034aqueous extractepilepsyAlzheimer’s diseaseantihemolytic effect |
| spellingShingle | Cicera Janaine Camilo Débora Odília Duarte Leite Natália Kelly Gomes de Carvalho José Walber Gonçalves Castro Jane Eire Silva Alencar de Menezes Helcio Silva dos Santos Joanda Paolla Raimundo Silva Daniela Ribeiro Alves Selene Maia de Morais Fabíola Fernandes Galvão Rodrigues José Galberto Martins da Costa Anticonvulsant, Anticholinesterase and Cytoprotective Effects of the Aqueous Extract of <i>Lippia sidoides</i> Cham Plants aqueous extract epilepsy Alzheimer’s disease antihemolytic effect |
| title | Anticonvulsant, Anticholinesterase and Cytoprotective Effects of the Aqueous Extract of <i>Lippia sidoides</i> Cham |
| title_full | Anticonvulsant, Anticholinesterase and Cytoprotective Effects of the Aqueous Extract of <i>Lippia sidoides</i> Cham |
| title_fullStr | Anticonvulsant, Anticholinesterase and Cytoprotective Effects of the Aqueous Extract of <i>Lippia sidoides</i> Cham |
| title_full_unstemmed | Anticonvulsant, Anticholinesterase and Cytoprotective Effects of the Aqueous Extract of <i>Lippia sidoides</i> Cham |
| title_short | Anticonvulsant, Anticholinesterase and Cytoprotective Effects of the Aqueous Extract of <i>Lippia sidoides</i> Cham |
| title_sort | anticonvulsant anticholinesterase and cytoprotective effects of the aqueous extract of i lippia sidoides i cham |
| topic | aqueous extract epilepsy Alzheimer’s disease antihemolytic effect |
| url | https://www.mdpi.com/2223-7747/13/21/3034 |
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