Do not let perfect be the enemy of good: the current reality of Bordetella testing
ABSTRACT Diagnosis of Bordetella pertussis is made by using polymerase chain reaction (PCR) to detect insertion sequence 481 (IS481). However, IS481 is found in both B. pertussis and Bordetella holmesii. In a recent study, Cole et al. (Microbiol Spectr 12:e00783-24. https://doi.org/10.1128/spectrum....
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American Society for Microbiology
2025-01-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02210-24 |
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| author | Rebecca Yee |
| author_facet | Rebecca Yee |
| author_sort | Rebecca Yee |
| collection | DOAJ |
| description | ABSTRACT Diagnosis of Bordetella pertussis is made by using polymerase chain reaction (PCR) to detect insertion sequence 481 (IS481). However, IS481 is found in both B. pertussis and Bordetella holmesii. In a recent study, Cole et al. (Microbiol Spectr 12:e00783-24. https://doi.org/10.1128/spectrum.00783-24) compared the accuracy of assays performed at two different commercial laboratories that used IS481 as a molecular target against a multiplex PCR assay performed at the Centers for Disease Control and Prevention (CDC) that can differentiate the different Bordetella species. The results demonstrated that the overall agreement among the assays from the commercial laboratories and the CDC was 85%. Only a small percentage (1.4%) of specimens positive for IS481 were identified as B. holmesii. This study suggests that PCR tests using IS481 as a target provide reliable diagnosis for pertussis. However, end users should recognize the limitations of PCR testing for pertussis including the inability to differentiate and monitor transmission of non-pertussis Bordetella species. |
| format | Article |
| id | doaj-art-4ca19220ba8340a8b053aa3020487134 |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj-art-4ca19220ba8340a8b053aa30204871342025-01-07T14:05:18ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-01-0113110.1128/spectrum.02210-24Do not let perfect be the enemy of good: the current reality of Bordetella testingRebecca Yee0Department of Pathology, George Washington University School of Medicine and Health Sciences, Washington, DC, USAABSTRACT Diagnosis of Bordetella pertussis is made by using polymerase chain reaction (PCR) to detect insertion sequence 481 (IS481). However, IS481 is found in both B. pertussis and Bordetella holmesii. In a recent study, Cole et al. (Microbiol Spectr 12:e00783-24. https://doi.org/10.1128/spectrum.00783-24) compared the accuracy of assays performed at two different commercial laboratories that used IS481 as a molecular target against a multiplex PCR assay performed at the Centers for Disease Control and Prevention (CDC) that can differentiate the different Bordetella species. The results demonstrated that the overall agreement among the assays from the commercial laboratories and the CDC was 85%. Only a small percentage (1.4%) of specimens positive for IS481 were identified as B. holmesii. This study suggests that PCR tests using IS481 as a target provide reliable diagnosis for pertussis. However, end users should recognize the limitations of PCR testing for pertussis including the inability to differentiate and monitor transmission of non-pertussis Bordetella species.https://journals.asm.org/doi/10.1128/spectrum.02210-24Bordetella real time-PCRnon-pertussis Bordetellapertussis diagnosticspertussis surveillance |
| spellingShingle | Rebecca Yee Do not let perfect be the enemy of good: the current reality of Bordetella testing Microbiology Spectrum Bordetella real time-PCR non-pertussis Bordetella pertussis diagnostics pertussis surveillance |
| title | Do not let perfect be the enemy of good: the current reality of Bordetella testing |
| title_full | Do not let perfect be the enemy of good: the current reality of Bordetella testing |
| title_fullStr | Do not let perfect be the enemy of good: the current reality of Bordetella testing |
| title_full_unstemmed | Do not let perfect be the enemy of good: the current reality of Bordetella testing |
| title_short | Do not let perfect be the enemy of good: the current reality of Bordetella testing |
| title_sort | do not let perfect be the enemy of good the current reality of bordetella testing |
| topic | Bordetella real time-PCR non-pertussis Bordetella pertussis diagnostics pertussis surveillance |
| url | https://journals.asm.org/doi/10.1128/spectrum.02210-24 |
| work_keys_str_mv | AT rebeccayee donotletperfectbetheenemyofgoodthecurrentrealityofbordetellatesting |