Improving enzymatic properties of BlTDH from Bacillus licheniformis through site-directed mutagenesis
L-threonine dehydrogenase (TDH) is a rate-limiting enzyme in the biosynthesis pathway of 2,5-dimethylpyrazine and 2,3,5-trimethylpyrazine, which are widely used food additives. However, natural TDH enzymes suffer from low catalytic activity and poor environmental adaptability, limiting their industr...
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| Main Authors: | Xun Liu, Hongyi Gu, Han Li, Shuanglian Chen, Zhen Tang, Wenli Quan |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-12-01
|
| Series: | Food Chemistry: Molecular Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666566225000334 |
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