Mechanisms of Cannabis Growth Promotion by <i>Bacillus velezensis</i> S141
<i>Cannabis sativa</i> L. has a variety of uses, including fiber production, food, oil, and medicine. In response to environmental concerns regarding chemical fertilizers, <i>Bacillus velezensis</i> S141 was examined as a plant-growth-promoting bacterium (PGPB) for cannabis....
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| Language: | English |
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MDPI AG
2024-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/13/21/2971 |
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| author | Phirom Aunkam Surachat Sibponkrung Sirawich Limkul Tuangrak Seabkongseng Kanjana Mahanil Kamolchanok Umnajkitikorn Nantakorn Boonkerd Neung Teaumroong Shusei Sato Panlada Tittabutr Pakpoom Boonchuen |
| author_facet | Phirom Aunkam Surachat Sibponkrung Sirawich Limkul Tuangrak Seabkongseng Kanjana Mahanil Kamolchanok Umnajkitikorn Nantakorn Boonkerd Neung Teaumroong Shusei Sato Panlada Tittabutr Pakpoom Boonchuen |
| author_sort | Phirom Aunkam |
| collection | DOAJ |
| description | <i>Cannabis sativa</i> L. has a variety of uses, including fiber production, food, oil, and medicine. In response to environmental concerns regarding chemical fertilizers, <i>Bacillus velezensis</i> S141 was examined as a plant-growth-promoting bacterium (PGPB) for cannabis. This study evaluated the effects of S141 on cannabis growth and utilized transcriptomic analysis to identify the responsive pathways. Inoculation with S141 significantly increased growth in laboratory and field environments, with most of the bacteria residing in the leaves, followed by the stems and roots, as determined by quantitative polymerase chain reaction (qPCR). Transcriptomic analysis revealed 976 differentially expressed genes. Upregulated genes were associated with metabolism, cellular processes, and catalytic activities, especially in the biosynthesis of phenylpropanoid, plant–pathogen interactions, and hormone signaling pathways. S141 mutants deficient in the production of auxin and cytokinin displayed reduced growth enhancement, which affirmed the roles of these hormones in cannabis development. These findings emphasize the potential of S141 as a sustainable growth promoter for cannabis and provide insights into the underlying pathways it influences. |
| format | Article |
| id | doaj-art-4c21a19d1ca54dbf96e68704d16f3f72 |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-4c21a19d1ca54dbf96e68704d16f3f722024-11-08T14:39:07ZengMDPI AGPlants2223-77472024-10-011321297110.3390/plants13212971Mechanisms of Cannabis Growth Promotion by <i>Bacillus velezensis</i> S141Phirom Aunkam0Surachat Sibponkrung1Sirawich Limkul2Tuangrak Seabkongseng3Kanjana Mahanil4Kamolchanok Umnajkitikorn5Nantakorn Boonkerd6Neung Teaumroong7Shusei Sato8Panlada Tittabutr9Pakpoom Boonchuen10School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandCenter of Excellent in Agricultural Product Innovation, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandInstitute of Research and Development, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandGraduate School of Life Sciences, Tohoku University, Sendai 980-8577, JapanSchool of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, ThailandSchool of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand<i>Cannabis sativa</i> L. has a variety of uses, including fiber production, food, oil, and medicine. In response to environmental concerns regarding chemical fertilizers, <i>Bacillus velezensis</i> S141 was examined as a plant-growth-promoting bacterium (PGPB) for cannabis. This study evaluated the effects of S141 on cannabis growth and utilized transcriptomic analysis to identify the responsive pathways. Inoculation with S141 significantly increased growth in laboratory and field environments, with most of the bacteria residing in the leaves, followed by the stems and roots, as determined by quantitative polymerase chain reaction (qPCR). Transcriptomic analysis revealed 976 differentially expressed genes. Upregulated genes were associated with metabolism, cellular processes, and catalytic activities, especially in the biosynthesis of phenylpropanoid, plant–pathogen interactions, and hormone signaling pathways. S141 mutants deficient in the production of auxin and cytokinin displayed reduced growth enhancement, which affirmed the roles of these hormones in cannabis development. These findings emphasize the potential of S141 as a sustainable growth promoter for cannabis and provide insights into the underlying pathways it influences.https://www.mdpi.com/2223-7747/13/21/2971<i>Cannabis sativa</i> L.<i>Bacillus velezensis</i> S141plant-growth-promoting bacterium (PGPB) |
| spellingShingle | Phirom Aunkam Surachat Sibponkrung Sirawich Limkul Tuangrak Seabkongseng Kanjana Mahanil Kamolchanok Umnajkitikorn Nantakorn Boonkerd Neung Teaumroong Shusei Sato Panlada Tittabutr Pakpoom Boonchuen Mechanisms of Cannabis Growth Promotion by <i>Bacillus velezensis</i> S141 Plants <i>Cannabis sativa</i> L. <i>Bacillus velezensis</i> S141 plant-growth-promoting bacterium (PGPB) |
| title | Mechanisms of Cannabis Growth Promotion by <i>Bacillus velezensis</i> S141 |
| title_full | Mechanisms of Cannabis Growth Promotion by <i>Bacillus velezensis</i> S141 |
| title_fullStr | Mechanisms of Cannabis Growth Promotion by <i>Bacillus velezensis</i> S141 |
| title_full_unstemmed | Mechanisms of Cannabis Growth Promotion by <i>Bacillus velezensis</i> S141 |
| title_short | Mechanisms of Cannabis Growth Promotion by <i>Bacillus velezensis</i> S141 |
| title_sort | mechanisms of cannabis growth promotion by i bacillus velezensis i s141 |
| topic | <i>Cannabis sativa</i> L. <i>Bacillus velezensis</i> S141 plant-growth-promoting bacterium (PGPB) |
| url | https://www.mdpi.com/2223-7747/13/21/2971 |
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