The repressor PrtR1 and the global H-NS-like regulators MvaT and MvaV enable the fine-tuning of R-tailocin expression in Pseudomonas protegens
Abstract Background Bacteria rely on an arsenal of weapons to challenge their opponents in highly competitive environments. To specifically counter closely related bacteria, specialized weapons with a narrow activity spectrum are deployed, particularly contractile phage tail-like particles or R-tail...
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2025-05-01
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| Online Access: | https://doi.org/10.1186/s12866-025-03983-9 |
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| author | Clara Margot Heiman Hammam Antar Florian Fournes Christoph Keel Jordan Vacheron |
| author_facet | Clara Margot Heiman Hammam Antar Florian Fournes Christoph Keel Jordan Vacheron |
| author_sort | Clara Margot Heiman |
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| description | Abstract Background Bacteria rely on an arsenal of weapons to challenge their opponents in highly competitive environments. To specifically counter closely related bacteria, specialized weapons with a narrow activity spectrum are deployed, particularly contractile phage tail-like particles or R-tailocins. Their production leads to the lysis of the producing cells, indicating that their expression must be carefully orchestrated so that only a small percentage of cells produce R-tailocins for the benefit of the entire population. Results In this study, we set out to better understand how the production of these phage tail-like weapons is regulated in environmental pseudomonads using the competitive plant root colonizer and environmental model strain Pseudomonas protegens CHA0. Using an RNA sequencing (RNA-seq) approach, we found that genes involved in DNA repair, particularly the SOS response program, are upregulated following exposure of the pseudomonad to the DNA-damaging agents mitomycin C and hydrogen peroxide, while genes involved in cell division and primary metabolism are downregulated. The R-tailocin and prophage gene clusters were also upregulated in response to these DNA damaging agents. By combining reverse genetics, transcriptional reporters and chromatin immunoprecipitation sequencing (ChIP-seq), we show that the R-tailocin locus-specific LexA-like regulator PrtR1 represses R-tailocin gene expression by binding directly to the promoter region of the cluster, while the histone-like nucleoid structuring (H-NS) proteins MvaT and MvaV act as master regulators that indirectly regulate R-tailocin cluster expression. Conclusion Our results suggest that at least these three regulators operate in concert to ensure tight control of R-tailocin expression and cell lytic release in environmental Pseudomonas protegens strains. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-05-01 |
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| series | BMC Microbiology |
| spelling | doaj-art-4bf0f9778db242b384e6e5a8d7e8ab4b2025-08-20T03:53:11ZengBMCBMC Microbiology1471-21802025-05-0125111610.1186/s12866-025-03983-9The repressor PrtR1 and the global H-NS-like regulators MvaT and MvaV enable the fine-tuning of R-tailocin expression in Pseudomonas protegensClara Margot Heiman0Hammam Antar1Florian Fournes2Christoph Keel3Jordan Vacheron4Department of Fundamental Microbiology, University of LausanneDepartment of Fundamental Microbiology, University of LausanneDepartment of Fundamental Microbiology, University of LausanneDepartment of Fundamental Microbiology, University of LausanneDepartment of Fundamental Microbiology, University of LausanneAbstract Background Bacteria rely on an arsenal of weapons to challenge their opponents in highly competitive environments. To specifically counter closely related bacteria, specialized weapons with a narrow activity spectrum are deployed, particularly contractile phage tail-like particles or R-tailocins. Their production leads to the lysis of the producing cells, indicating that their expression must be carefully orchestrated so that only a small percentage of cells produce R-tailocins for the benefit of the entire population. Results In this study, we set out to better understand how the production of these phage tail-like weapons is regulated in environmental pseudomonads using the competitive plant root colonizer and environmental model strain Pseudomonas protegens CHA0. Using an RNA sequencing (RNA-seq) approach, we found that genes involved in DNA repair, particularly the SOS response program, are upregulated following exposure of the pseudomonad to the DNA-damaging agents mitomycin C and hydrogen peroxide, while genes involved in cell division and primary metabolism are downregulated. The R-tailocin and prophage gene clusters were also upregulated in response to these DNA damaging agents. By combining reverse genetics, transcriptional reporters and chromatin immunoprecipitation sequencing (ChIP-seq), we show that the R-tailocin locus-specific LexA-like regulator PrtR1 represses R-tailocin gene expression by binding directly to the promoter region of the cluster, while the histone-like nucleoid structuring (H-NS) proteins MvaT and MvaV act as master regulators that indirectly regulate R-tailocin cluster expression. Conclusion Our results suggest that at least these three regulators operate in concert to ensure tight control of R-tailocin expression and cell lytic release in environmental Pseudomonas protegens strains.https://doi.org/10.1186/s12866-025-03983-9TailocinPyocinRegulationPseudomonasH-NS regulatorsSOS response |
| spellingShingle | Clara Margot Heiman Hammam Antar Florian Fournes Christoph Keel Jordan Vacheron The repressor PrtR1 and the global H-NS-like regulators MvaT and MvaV enable the fine-tuning of R-tailocin expression in Pseudomonas protegens BMC Microbiology Tailocin Pyocin Regulation Pseudomonas H-NS regulators SOS response |
| title | The repressor PrtR1 and the global H-NS-like regulators MvaT and MvaV enable the fine-tuning of R-tailocin expression in Pseudomonas protegens |
| title_full | The repressor PrtR1 and the global H-NS-like regulators MvaT and MvaV enable the fine-tuning of R-tailocin expression in Pseudomonas protegens |
| title_fullStr | The repressor PrtR1 and the global H-NS-like regulators MvaT and MvaV enable the fine-tuning of R-tailocin expression in Pseudomonas protegens |
| title_full_unstemmed | The repressor PrtR1 and the global H-NS-like regulators MvaT and MvaV enable the fine-tuning of R-tailocin expression in Pseudomonas protegens |
| title_short | The repressor PrtR1 and the global H-NS-like regulators MvaT and MvaV enable the fine-tuning of R-tailocin expression in Pseudomonas protegens |
| title_sort | repressor prtr1 and the global h ns like regulators mvat and mvav enable the fine tuning of r tailocin expression in pseudomonas protegens |
| topic | Tailocin Pyocin Regulation Pseudomonas H-NS regulators SOS response |
| url | https://doi.org/10.1186/s12866-025-03983-9 |
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