Safety and Immunogenicity of the Live Attenuated Vaccine QazCOVID-Live Against Coronavirus Infection COVID-19: Pre-Clinical Study Results

The research conducted in this preclinical study assesses QazCovid-live, a live attenuated COVID-19 vaccine created in Kazakhstan, by conducting preclinical evaluations of safety, immunogenicity, and allergenicity in various animal models, including mice, rats, hamsters, and guinea pigs. The vaccine...

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Main Authors: Lespek Kutumbetov, Balzhan Myrzakhmetova, Aiganym Tussipova, Gulzhan Zhapparova, Talshyngul Tlenchiyeva, Karina Bissenbayeva, Kuanysh Zhapar, Kuandyk Zhugunissov, Sergazy Nurabayev, Aslan Kerimbayev
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Vaccines
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Online Access:https://www.mdpi.com/2076-393X/12/12/1401
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author Lespek Kutumbetov
Balzhan Myrzakhmetova
Aiganym Tussipova
Gulzhan Zhapparova
Talshyngul Tlenchiyeva
Karina Bissenbayeva
Kuanysh Zhapar
Kuandyk Zhugunissov
Sergazy Nurabayev
Aslan Kerimbayev
author_facet Lespek Kutumbetov
Balzhan Myrzakhmetova
Aiganym Tussipova
Gulzhan Zhapparova
Talshyngul Tlenchiyeva
Karina Bissenbayeva
Kuanysh Zhapar
Kuandyk Zhugunissov
Sergazy Nurabayev
Aslan Kerimbayev
author_sort Lespek Kutumbetov
collection DOAJ
description The research conducted in this preclinical study assesses QazCovid-live, a live attenuated COVID-19 vaccine created in Kazakhstan, by conducting preclinical evaluations of safety, immunogenicity, and allergenicity in various animal models, including mice, rats, hamsters, and guinea pigs. The vaccine, developed by attenuating SARS-CoV-2 via numerous Vero cell passages, had no significant adverse effects in acute and subacute toxicity assessments, even at elevated dosages. Allergenicity testing indicated the absence of both immediate and delayed hypersensitivity reactions. Immunogenicity evaluations revealed strong virus-neutralizing antibody responses, especially following intranasal and intratracheal delivery. Studies on reversibility and transmission further validated the vaccine’s stability and non-pathogenicity. The data indicate that QazCovid-live is safe, immunogenic, and prepared for clinical trials, presenting a potential strategy for COVID-19 prevention.
format Article
id doaj-art-4be9b2f0ea0e4628be51ce27a72cd9a8
institution Kabale University
issn 2076-393X
language English
publishDate 2024-12-01
publisher MDPI AG
record_format Article
series Vaccines
spelling doaj-art-4be9b2f0ea0e4628be51ce27a72cd9a82024-12-27T14:58:06ZengMDPI AGVaccines2076-393X2024-12-011212140110.3390/vaccines12121401Safety and Immunogenicity of the Live Attenuated Vaccine QazCOVID-Live Against Coronavirus Infection COVID-19: Pre-Clinical Study ResultsLespek Kutumbetov0Balzhan Myrzakhmetova1Aiganym Tussipova2Gulzhan Zhapparova3Talshyngul Tlenchiyeva4Karina Bissenbayeva5Kuanysh Zhapar6Kuandyk Zhugunissov7Sergazy Nurabayev8Aslan Kerimbayev9Research Institute for Biological Safety Problems, Gvardeiskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, Gvardeiskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, Gvardeiskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, Gvardeiskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, Gvardeiskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, Gvardeiskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, Gvardeiskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, Gvardeiskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, Gvardeiskiy 080409, KazakhstanResearch Institute for Biological Safety Problems, Gvardeiskiy 080409, KazakhstanThe research conducted in this preclinical study assesses QazCovid-live, a live attenuated COVID-19 vaccine created in Kazakhstan, by conducting preclinical evaluations of safety, immunogenicity, and allergenicity in various animal models, including mice, rats, hamsters, and guinea pigs. The vaccine, developed by attenuating SARS-CoV-2 via numerous Vero cell passages, had no significant adverse effects in acute and subacute toxicity assessments, even at elevated dosages. Allergenicity testing indicated the absence of both immediate and delayed hypersensitivity reactions. Immunogenicity evaluations revealed strong virus-neutralizing antibody responses, especially following intranasal and intratracheal delivery. Studies on reversibility and transmission further validated the vaccine’s stability and non-pathogenicity. The data indicate that QazCovid-live is safe, immunogenic, and prepared for clinical trials, presenting a potential strategy for COVID-19 prevention.https://www.mdpi.com/2076-393X/12/12/1401QazCOVID-livelive attenuated vaccineSARS-CoV-2COVID-19 preventionpreclinical studyvaccine safety
spellingShingle Lespek Kutumbetov
Balzhan Myrzakhmetova
Aiganym Tussipova
Gulzhan Zhapparova
Talshyngul Tlenchiyeva
Karina Bissenbayeva
Kuanysh Zhapar
Kuandyk Zhugunissov
Sergazy Nurabayev
Aslan Kerimbayev
Safety and Immunogenicity of the Live Attenuated Vaccine QazCOVID-Live Against Coronavirus Infection COVID-19: Pre-Clinical Study Results
Vaccines
QazCOVID-live
live attenuated vaccine
SARS-CoV-2
COVID-19 prevention
preclinical study
vaccine safety
title Safety and Immunogenicity of the Live Attenuated Vaccine QazCOVID-Live Against Coronavirus Infection COVID-19: Pre-Clinical Study Results
title_full Safety and Immunogenicity of the Live Attenuated Vaccine QazCOVID-Live Against Coronavirus Infection COVID-19: Pre-Clinical Study Results
title_fullStr Safety and Immunogenicity of the Live Attenuated Vaccine QazCOVID-Live Against Coronavirus Infection COVID-19: Pre-Clinical Study Results
title_full_unstemmed Safety and Immunogenicity of the Live Attenuated Vaccine QazCOVID-Live Against Coronavirus Infection COVID-19: Pre-Clinical Study Results
title_short Safety and Immunogenicity of the Live Attenuated Vaccine QazCOVID-Live Against Coronavirus Infection COVID-19: Pre-Clinical Study Results
title_sort safety and immunogenicity of the live attenuated vaccine qazcovid live against coronavirus infection covid 19 pre clinical study results
topic QazCOVID-live
live attenuated vaccine
SARS-CoV-2
COVID-19 prevention
preclinical study
vaccine safety
url https://www.mdpi.com/2076-393X/12/12/1401
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