Optimized molecule detection in localization microscopy with selected false positive probability

Abstract Single-molecule localization microscopy (SMLM) allows imaging beyond the diffraction limit. Detection of molecules is a crucial initial step in SMLM. False positive detections, which are not quantitatively controlled in current methods, are a source of artifacts that affect the entire SMLM...

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Bibliographic Details
Main Authors: Miroslav Hekrdla, David Roesel, Niklas Hansen, Soumya Frederick, Khalilullah Umar, Vladimíra Petráková
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-55952-5
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