Optimized molecule detection in localization microscopy with selected false positive probability
Abstract Single-molecule localization microscopy (SMLM) allows imaging beyond the diffraction limit. Detection of molecules is a crucial initial step in SMLM. False positive detections, which are not quantitatively controlled in current methods, are a source of artifacts that affect the entire SMLM...
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Main Authors: | Miroslav Hekrdla, David Roesel, Niklas Hansen, Soumya Frederick, Khalilullah Umar, Vladimíra Petráková |
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Format: | Article |
Language: | English |
Published: |
Nature Portfolio
2025-01-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-025-55952-5 |
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