The development of a method for determining the biological activity of erythropoietin preparations in vitro
The method for the assessment of biological activity of erythropoietin preparations in vitro on TF-1 sensitive cells culture has been developed. The special characteristics of measurements and calculations of the biological activity of erythropoietin with different levels of glycosylation in vivo an...
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| Format: | Article |
| Language: | Russian |
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Ministry of Health of the Russian Federation. Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products»
2018-02-01
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| Series: | Биопрепараты: Профилактика, диагностика, лечение |
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| Online Access: | https://www.biopreparations.ru/jour/article/view/52 |
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| _version_ | 1849342842683523072 |
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| author | N. A. Gavrilova S. A. Cherepushkin N. V. Rykalina Yu. I. Obukhov |
| author_facet | N. A. Gavrilova S. A. Cherepushkin N. V. Rykalina Yu. I. Obukhov |
| author_sort | N. A. Gavrilova |
| collection | DOAJ |
| description | The method for the assessment of biological activity of erythropoietin preparations in vitro on TF-1 sensitive cells culture has been developed. The special characteristics of measurements and calculations of the biological activity of erythropoietin with different levels of glycosylation in vivo and in vitro. Linear response ranges for cell lines to erythropoietin and its hyperglycosylated analogue differ and make (0.39-0.56 ng/ml) and (3.1-12.5 ng/ml), respectively. The results have shown the possibility of using cellular test for comparing and assaying the activity of erythropoietin analogues in standard international units of biological activity (IU) set for erythropoietin. |
| format | Article |
| id | doaj-art-4b97b448c2a94e89b078c7cb3b7859c5 |
| institution | Kabale University |
| issn | 2221-996X 2619-1156 |
| language | Russian |
| publishDate | 2018-02-01 |
| publisher | Ministry of Health of the Russian Federation. Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products» |
| record_format | Article |
| series | Биопрепараты: Профилактика, диагностика, лечение |
| spelling | doaj-art-4b97b448c2a94e89b078c7cb3b7859c52025-08-20T03:43:15ZrusMinistry of Health of the Russian Federation. Federal State Budgetary Institution «Scientific Centre for Expert Evaluation of Medicinal Products»Биопрепараты: Профилактика, диагностика, лечение2221-996X2619-11562018-02-0116212012452The development of a method for determining the biological activity of erythropoietin preparations in vitroN. A. Gavrilova0S. A. Cherepushkin1N. V. Rykalina2Yu. I. Obukhov3Scientific Centre for Expert Evaluation of Medicinal ProductsState Research Institute of Selection of Industrial MicroorganismsState Research Institute of Selection of Industrial MicroorganismsScientific Centre for Expert Evaluation of Medicinal ProductsThe method for the assessment of biological activity of erythropoietin preparations in vitro on TF-1 sensitive cells culture has been developed. The special characteristics of measurements and calculations of the biological activity of erythropoietin with different levels of glycosylation in vivo and in vitro. Linear response ranges for cell lines to erythropoietin and its hyperglycosylated analogue differ and make (0.39-0.56 ng/ml) and (3.1-12.5 ng/ml), respectively. The results have shown the possibility of using cellular test for comparing and assaying the activity of erythropoietin analogues in standard international units of biological activity (IU) set for erythropoietin.https://www.biopreparations.ru/jour/article/view/52биологическая активностьэритропоэтиндарбэпоэтинклеточная линия tf-1пролиферативный ответbiological activityerythropoietindarbepoetincell line tf-1proliferative response |
| spellingShingle | N. A. Gavrilova S. A. Cherepushkin N. V. Rykalina Yu. I. Obukhov The development of a method for determining the biological activity of erythropoietin preparations in vitro Биопрепараты: Профилактика, диагностика, лечение биологическая активность эритропоэтин дарбэпоэтин клеточная линия tf-1 пролиферативный ответ biological activity erythropoietin darbepoetin cell line tf-1 proliferative response |
| title | The development of a method for determining the biological activity of erythropoietin preparations in vitro |
| title_full | The development of a method for determining the biological activity of erythropoietin preparations in vitro |
| title_fullStr | The development of a method for determining the biological activity of erythropoietin preparations in vitro |
| title_full_unstemmed | The development of a method for determining the biological activity of erythropoietin preparations in vitro |
| title_short | The development of a method for determining the biological activity of erythropoietin preparations in vitro |
| title_sort | development of a method for determining the biological activity of erythropoietin preparations in vitro |
| topic | биологическая активность эритропоэтин дарбэпоэтин клеточная линия tf-1 пролиферативный ответ biological activity erythropoietin darbepoetin cell line tf-1 proliferative response |
| url | https://www.biopreparations.ru/jour/article/view/52 |
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