Integrity of RNA in long-term-stored cervical liquid-based cytology samples: implications for biomarker research
Biobanks of cervical screening (LBC) samples annotated with disease status are an invaluable resource to support the development of tools for the risk stratification of disease. Although there is growing interest in the assessment of RNA-based biomarkers, little is known on the suitability and durab...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-06-01
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| Series: | BioTechniques |
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| Online Access: | https://www.tandfonline.com/doi/10.2144/btn-2023-0112 |
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| _version_ | 1841526640462528512 |
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| author | Madeleine P J White Andrew Stevenson Hana Elasifer Chris Davis Kyriaki Nomikou Kate Cuschieri Sheila V Graham |
| author_facet | Madeleine P J White Andrew Stevenson Hana Elasifer Chris Davis Kyriaki Nomikou Kate Cuschieri Sheila V Graham |
| author_sort | Madeleine P J White |
| collection | DOAJ |
| description | Biobanks of cervical screening (LBC) samples annotated with disease status are an invaluable resource to support the development of tools for the risk stratification of disease. Although there is growing interest in the assessment of RNA-based biomarkers, little is known on the suitability and durability of stored clinical samples (commonly used in cervical screening) to support RNA-based research. RNA was extracted from 260 stored LBC samples. Storage at -80°C or -25°C allowed isolation of sufficient RNA for further analysis. RNA was found to be substantially degraded according to Agilent Bioanalyser data. Despite this, RT-qPCR was successful in 95% samples tested. These data suggest that biobanked LBC samples are suitable for RNA-based assessment even if stored for up to 14 years. |
| format | Article |
| id | doaj-art-4ae1e75d3a3c43cebe5a957ad6cf0ef9 |
| institution | Kabale University |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-4ae1e75d3a3c43cebe5a957ad6cf0ef92025-01-16T15:38:47ZengTaylor & Francis GroupBioTechniques0736-62051940-98182024-06-0176624525310.2144/btn-2023-0112Integrity of RNA in long-term-stored cervical liquid-based cytology samples: implications for biomarker researchMadeleine P J White0Andrew Stevenson1Hana Elasifer2Chris Davis3Kyriaki Nomikou4Kate Cuschieri5Sheila V Graham6Centre for Virus Research, School of Infection and Immunity, College of Medical, Veterinary & Life Sciences, University of Glasgow, Glasgow, UKCentre for Virus Research, School of Infection and Immunity, College of Medical, Veterinary & Life Sciences, University of Glasgow, Glasgow, UKScottish HPV Reference Laboratory, NHS Lothian, Edinburgh, UKCentre for Virus Research, School of Infection and Immunity, College of Medical, Veterinary & Life Sciences, University of Glasgow, Glasgow, UKCentre for Virus Research, School of Infection and Immunity, College of Medical, Veterinary & Life Sciences, University of Glasgow, Glasgow, UKScottish HPV Reference Laboratory, NHS Lothian, Edinburgh, UKCentre for Virus Research, School of Infection and Immunity, College of Medical, Veterinary & Life Sciences, University of Glasgow, Glasgow, UKBiobanks of cervical screening (LBC) samples annotated with disease status are an invaluable resource to support the development of tools for the risk stratification of disease. Although there is growing interest in the assessment of RNA-based biomarkers, little is known on the suitability and durability of stored clinical samples (commonly used in cervical screening) to support RNA-based research. RNA was extracted from 260 stored LBC samples. Storage at -80°C or -25°C allowed isolation of sufficient RNA for further analysis. RNA was found to be substantially degraded according to Agilent Bioanalyser data. Despite this, RT-qPCR was successful in 95% samples tested. These data suggest that biobanked LBC samples are suitable for RNA-based assessment even if stored for up to 14 years.https://www.tandfonline.com/doi/10.2144/btn-2023-0112biobankingcervical screeningreverse transcriptase quantitative PCRRNA biomarkersRNA qualitystorage temperature |
| spellingShingle | Madeleine P J White Andrew Stevenson Hana Elasifer Chris Davis Kyriaki Nomikou Kate Cuschieri Sheila V Graham Integrity of RNA in long-term-stored cervical liquid-based cytology samples: implications for biomarker research BioTechniques biobanking cervical screening reverse transcriptase quantitative PCR RNA biomarkers RNA quality storage temperature |
| title | Integrity of RNA in long-term-stored cervical liquid-based cytology samples: implications for biomarker research |
| title_full | Integrity of RNA in long-term-stored cervical liquid-based cytology samples: implications for biomarker research |
| title_fullStr | Integrity of RNA in long-term-stored cervical liquid-based cytology samples: implications for biomarker research |
| title_full_unstemmed | Integrity of RNA in long-term-stored cervical liquid-based cytology samples: implications for biomarker research |
| title_short | Integrity of RNA in long-term-stored cervical liquid-based cytology samples: implications for biomarker research |
| title_sort | integrity of rna in long term stored cervical liquid based cytology samples implications for biomarker research |
| topic | biobanking cervical screening reverse transcriptase quantitative PCR RNA biomarkers RNA quality storage temperature |
| url | https://www.tandfonline.com/doi/10.2144/btn-2023-0112 |
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