Protocol for generating a 3D culture of epiblast stem cells

Protocol for generating a 3D culture of epiblast stem cells

Summary: Mouse gastrulation entails concomitant changes in cell fate, tissue shape, and embryo size. The use of a reproducible in vitro system is crucial for dissecting the mechanisms that coordinate these events. Here, we present a protocol for generating a 3D culture of epiblast stem cells (3D Epi...

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Bibliographic Details
Main Authors: Viviane S. Rosa, Nanami Sato, Marta N. Shahbazi
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:STAR Protocols
Subjects:
Developmental biology
Stem Cells
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724005124
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