Unraveling Tissue‐Specific Fatty Acid Biosynthesis and Inter‐Tissue Crosstalk in Mice through Stable‐Isotope Tracing Metabolomics
Abstract Biosynthesis of free fatty acids (FFAs) in mammals is pivotal for metabolic homeostasis, yet a comprehensive understanding of tissue‐specific biosynthesis and inter‐tissue crosstalk of FFAs remains incomplete. In vivo stable‐isotope tracing metabolomics is utilized to comprehensively measur...
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| Format: | Article |
| Language: | English |
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Wiley
2025-08-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202503662 |
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| author | Beizi Xing Ruohong Wang Tianzhang Kou Wenbin Liu Zheng‐Jiang Zhu |
| author_facet | Beizi Xing Ruohong Wang Tianzhang Kou Wenbin Liu Zheng‐Jiang Zhu |
| author_sort | Beizi Xing |
| collection | DOAJ |
| description | Abstract Biosynthesis of free fatty acids (FFAs) in mammals is pivotal for metabolic homeostasis, yet a comprehensive understanding of tissue‐specific biosynthesis and inter‐tissue crosstalk of FFAs remains incomplete. In vivo stable‐isotope tracing metabolomics is utilized to comprehensively measure FFA biosynthesis and inter‐tissue crosstalk in mice. Systematically assessing tissue‐specific biosynthesis of 13 FFAs across 15 tissues unveils dynamic spatial and temporal accumulation and redistribution of FFAs throughout the body. Employing an analytical framework to deconvolve mass isotopologue patterns, inter‐tissue crosstalk is explored for saturated, polyunsaturated, and monounsaturated FFAs, and quantify communications of FFA (16:0) and FFA (18:0) between the liver and other tissues. Then, a decline in fatty acid biosynthesis in peripheral tissues but not in the brain of aged mice is observed, particularly evident in palmitic acid and monounsaturated fatty acids. These findings illuminate the complex interplay between tissue‐specific fatty acid biosynthesis and the maintenance of metabolic homeostasis. |
| format | Article |
| id | doaj-art-4a756a60e69f40cabee775ada2624f8c |
| institution | Kabale University |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-4a756a60e69f40cabee775ada2624f8c2025-08-23T14:14:06ZengWileyAdvanced Science2198-38442025-08-011231n/an/a10.1002/advs.202503662Unraveling Tissue‐Specific Fatty Acid Biosynthesis and Inter‐Tissue Crosstalk in Mice through Stable‐Isotope Tracing MetabolomicsBeizi Xing0Ruohong Wang1Tianzhang Kou2Wenbin Liu3Zheng‐Jiang Zhu4Interdisciplinary Research Center on Biology and Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai 200032 ChinaInterdisciplinary Research Center on Biology and Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai 200032 ChinaInterdisciplinary Research Center on Biology and Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai 200032 ChinaInterdisciplinary Research Center on Biology and Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai 200032 ChinaInterdisciplinary Research Center on Biology and Chemistry Shanghai Institute of Organic Chemistry Chinese Academy of Sciences Shanghai 200032 ChinaAbstract Biosynthesis of free fatty acids (FFAs) in mammals is pivotal for metabolic homeostasis, yet a comprehensive understanding of tissue‐specific biosynthesis and inter‐tissue crosstalk of FFAs remains incomplete. In vivo stable‐isotope tracing metabolomics is utilized to comprehensively measure FFA biosynthesis and inter‐tissue crosstalk in mice. Systematically assessing tissue‐specific biosynthesis of 13 FFAs across 15 tissues unveils dynamic spatial and temporal accumulation and redistribution of FFAs throughout the body. Employing an analytical framework to deconvolve mass isotopologue patterns, inter‐tissue crosstalk is explored for saturated, polyunsaturated, and monounsaturated FFAs, and quantify communications of FFA (16:0) and FFA (18:0) between the liver and other tissues. Then, a decline in fatty acid biosynthesis in peripheral tissues but not in the brain of aged mice is observed, particularly evident in palmitic acid and monounsaturated fatty acids. These findings illuminate the complex interplay between tissue‐specific fatty acid biosynthesis and the maintenance of metabolic homeostasis.https://doi.org/10.1002/advs.202503662fatty acid biosynthesisinter‐tissue crosstalkmass spectrometrymetabolomicsstable‐isotope tracing |
| spellingShingle | Beizi Xing Ruohong Wang Tianzhang Kou Wenbin Liu Zheng‐Jiang Zhu Unraveling Tissue‐Specific Fatty Acid Biosynthesis and Inter‐Tissue Crosstalk in Mice through Stable‐Isotope Tracing Metabolomics Advanced Science fatty acid biosynthesis inter‐tissue crosstalk mass spectrometry metabolomics stable‐isotope tracing |
| title | Unraveling Tissue‐Specific Fatty Acid Biosynthesis and Inter‐Tissue Crosstalk in Mice through Stable‐Isotope Tracing Metabolomics |
| title_full | Unraveling Tissue‐Specific Fatty Acid Biosynthesis and Inter‐Tissue Crosstalk in Mice through Stable‐Isotope Tracing Metabolomics |
| title_fullStr | Unraveling Tissue‐Specific Fatty Acid Biosynthesis and Inter‐Tissue Crosstalk in Mice through Stable‐Isotope Tracing Metabolomics |
| title_full_unstemmed | Unraveling Tissue‐Specific Fatty Acid Biosynthesis and Inter‐Tissue Crosstalk in Mice through Stable‐Isotope Tracing Metabolomics |
| title_short | Unraveling Tissue‐Specific Fatty Acid Biosynthesis and Inter‐Tissue Crosstalk in Mice through Stable‐Isotope Tracing Metabolomics |
| title_sort | unraveling tissue specific fatty acid biosynthesis and inter tissue crosstalk in mice through stable isotope tracing metabolomics |
| topic | fatty acid biosynthesis inter‐tissue crosstalk mass spectrometry metabolomics stable‐isotope tracing |
| url | https://doi.org/10.1002/advs.202503662 |
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