Presence of Cryptosporidium spp and other enteroparasites with pathogenic potential in hemodialysis patients: an open controlled study
Abstract Introduction: The World Health Organization (WHO) points out that infection by enteroparasites can affect ~3.5 billion people around the world. Hemodialysis (HD) patients may be more susceptible to infections by opportunistic pathogens due to impaired immune function. We evaluated enteropa...
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Sociedade Brasileira de Nefrologia
2024-12-01
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| Series: | Brazilian Journal of Nephrology |
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| author | Yara Leite Adami Nycole Abreu Gama Flavia de Souza Cunha Regina Helena Saramago Peralta Jocemir Ronaldo Lugon |
| author_facet | Yara Leite Adami Nycole Abreu Gama Flavia de Souza Cunha Regina Helena Saramago Peralta Jocemir Ronaldo Lugon |
| author_sort | Yara Leite Adami |
| collection | DOAJ |
| description | Abstract Introduction: The World Health Organization (WHO) points out that infection by enteroparasites can affect ~3.5 billion people around the world. Hemodialysis (HD) patients may be more susceptible to infections by opportunistic pathogens due to impaired immune function. We evaluated enteroparasite infection in a sample of HD-patients from two dialysis centers and in a control group. Methods: Fecal samples were processed using the Hoffmann-Pons-Janner, Ritchie, Willis, and Rugai techniques. Patients with kidney failure from two dialysis centers undergoing HD for more than 3 months were included. The control group consisted of relatives of the patients without overt CKD. The TaqMan PCR and multiplex real-time PCR were carried out for detection of Cryptosporidium spp. and C. parvum and to differentiate the Entamoeba (E.) histolytica/E. dispar complex, respectively Results: A total of 97 HD patients and 42 controls were enrolled in the study. Fifty (51.5%) fecal samples from the HD group were positive for enteroparasites, as were 26 (61.9%) from the control group (P = 0.260). S. stercoralis was the single helminth detected and was only present in HD-patients. Coproscopy detected seven positive samples for the E. histolytica/E. dispar complex, three from HD patients and four from controls: by PCR, all samples were positive for the non-pathogenic E. dispar. Safranin-stained fecal smear slides were all negative for Cryptosporidium spp. However, by PCR, amplification for Crypstosporidium spp. was seen in six samples, all from the HD patients. Two of the species were classified as C. hominis by PCR-RFLP Conclusions: Enteroparasite infection as detected by traditional techniques were not more prevalent in HD patients, but S. stercoralis was only found in these patients. It is noteworthy that Cryptosporidium spp. infection, also affecting only HD patients, could only be detected by molecular biology techniques. |
| format | Article |
| id | doaj-art-4a5e35b841354dfbb010d96a57364986 |
| institution | Kabale University |
| issn | 2175-8239 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Sociedade Brasileira de Nefrologia |
| record_format | Article |
| series | Brazilian Journal of Nephrology |
| spelling | doaj-art-4a5e35b841354dfbb010d96a573649862025-01-07T07:39:00ZengSociedade Brasileira de NefrologiaBrazilian Journal of Nephrology2175-82392024-12-0147110.1590/2175-8239-jbn-2024-0015enPresence of Cryptosporidium spp and other enteroparasites with pathogenic potential in hemodialysis patients: an open controlled studyYara Leite Adamihttps://orcid.org/0000-0003-2881-923XNycole Abreu Gamahttps://orcid.org/0009-0007-8694-3200Flavia de Souza Cunhahttps://orcid.org/0000-0002-9899-1652Regina Helena Saramago Peraltahttps://orcid.org/0000-0002-7986-8202Jocemir Ronaldo Lugonhttps://orcid.org/0000-0001-6791-3910Abstract Introduction: The World Health Organization (WHO) points out that infection by enteroparasites can affect ~3.5 billion people around the world. Hemodialysis (HD) patients may be more susceptible to infections by opportunistic pathogens due to impaired immune function. We evaluated enteroparasite infection in a sample of HD-patients from two dialysis centers and in a control group. Methods: Fecal samples were processed using the Hoffmann-Pons-Janner, Ritchie, Willis, and Rugai techniques. Patients with kidney failure from two dialysis centers undergoing HD for more than 3 months were included. The control group consisted of relatives of the patients without overt CKD. The TaqMan PCR and multiplex real-time PCR were carried out for detection of Cryptosporidium spp. and C. parvum and to differentiate the Entamoeba (E.) histolytica/E. dispar complex, respectively Results: A total of 97 HD patients and 42 controls were enrolled in the study. Fifty (51.5%) fecal samples from the HD group were positive for enteroparasites, as were 26 (61.9%) from the control group (P = 0.260). S. stercoralis was the single helminth detected and was only present in HD-patients. Coproscopy detected seven positive samples for the E. histolytica/E. dispar complex, three from HD patients and four from controls: by PCR, all samples were positive for the non-pathogenic E. dispar. Safranin-stained fecal smear slides were all negative for Cryptosporidium spp. However, by PCR, amplification for Crypstosporidium spp. was seen in six samples, all from the HD patients. Two of the species were classified as C. hominis by PCR-RFLP Conclusions: Enteroparasite infection as detected by traditional techniques were not more prevalent in HD patients, but S. stercoralis was only found in these patients. It is noteworthy that Cryptosporidium spp. infection, also affecting only HD patients, could only be detected by molecular biology techniques.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002025000100303&lng=en&tlng=enRenal Insufficiency, ChronicRenal DialysisParasitesCryptosporidiumLaboratory Diagnosis |
| spellingShingle | Yara Leite Adami Nycole Abreu Gama Flavia de Souza Cunha Regina Helena Saramago Peralta Jocemir Ronaldo Lugon Presence of Cryptosporidium spp and other enteroparasites with pathogenic potential in hemodialysis patients: an open controlled study Brazilian Journal of Nephrology Renal Insufficiency, Chronic Renal Dialysis Parasites Cryptosporidium Laboratory Diagnosis |
| title | Presence of Cryptosporidium spp and other enteroparasites with pathogenic potential in hemodialysis patients: an open controlled study |
| title_full | Presence of Cryptosporidium spp and other enteroparasites with pathogenic potential in hemodialysis patients: an open controlled study |
| title_fullStr | Presence of Cryptosporidium spp and other enteroparasites with pathogenic potential in hemodialysis patients: an open controlled study |
| title_full_unstemmed | Presence of Cryptosporidium spp and other enteroparasites with pathogenic potential in hemodialysis patients: an open controlled study |
| title_short | Presence of Cryptosporidium spp and other enteroparasites with pathogenic potential in hemodialysis patients: an open controlled study |
| title_sort | presence of cryptosporidium spp and other enteroparasites with pathogenic potential in hemodialysis patients an open controlled study |
| topic | Renal Insufficiency, Chronic Renal Dialysis Parasites Cryptosporidium Laboratory Diagnosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002025000100303&lng=en&tlng=en |
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