High levels of type II Fusarium head blight resistance conferred in wheat by combining wheat gene Fhb1 with Lophopyrum elongatum gene Fhb7The2
Wheat Fusarium head blight (FHB) leads to losses of grain yield and quality. Ingestion of diseased grain is detrimental to human health due to the mycotoxins present in the grain. Developing resistant cultivars for environments where FHB is prevalent is therefore an important breeding objective. One...
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Czech Academy of Agricultural Sciences
2025-01-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
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| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-202501-0003_high-levels-of-type-ii-fusarium-head-blight-resistance-conferred-in-wheat-by-combining-wheat-gene-fhb1-with-lop.php |
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| author | Jan Dvorak Karin R. Deal Patrick E. McGuire Emily J. Conley James A. Anderson George Fedak Julia A. Malvick Han Chen Hans-Georg Müller |
| author_facet | Jan Dvorak Karin R. Deal Patrick E. McGuire Emily J. Conley James A. Anderson George Fedak Julia A. Malvick Han Chen Hans-Georg Müller |
| author_sort | Jan Dvorak |
| collection | DOAJ |
| description | Wheat Fusarium head blight (FHB) leads to losses of grain yield and quality. Ingestion of diseased grain is detrimental to human health due to the mycotoxins present in the grain. Developing resistant cultivars for environments where FHB is prevalent is therefore an important breeding objective. One of the most effective wheat genes conferring type II resistance to FHB is Fhb1, originally discovered in the Chinese cultivar (cv) Sumai 3. Another excellent FHB resistance gene is Fhb7 located on the long arm of Lophopyrum elongatum chromosome 7E. Several alleles of Fhb7 have been identified. Allele Fhb7The2 was found in disomic substitution lines 7E(7A), 7E(7B) and 7E(7D) derived from amphiploid AgCS. The amphiploid was produced from a hybrid Triticum aestivum cv Chinese Spring × L. elongatum. To find if combining Fhb7The2 with Fhb1 confers higher resistance in wheat than single genes, an introgression line derived from AgCS and possessing Fhb7The2 was recurrently backcrossed to bread wheat cv Rollag and MN-Washburn possessing Fhb1. Experimental lines possessing both Fhb7The2 and Fhb1 were developed and validated cytogenetically and with the L. elongatum genome-wide Sequenom SNP MassARRAY. Spikes of these lines, parental cv Rollag and MN-Washburn, and those of disomic addition line 7E possessing Fhb7The2 plus controls were inoculated with Fusarium in a twice-replicated trial in controlled greenhouse environmental conditions. FHB infection rates were significantly lower in lines combining Fhb7The2 with Fhb1 than in materials with Fhb7The2 or Fhb1 alone. |
| format | Article |
| id | doaj-art-4a57758a9d1a413aa1f66e40590c762a |
| institution | Kabale University |
| issn | 1212-1975 1805-9325 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj-art-4a57758a9d1a413aa1f66e40590c762a2025-01-14T12:46:40ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252025-01-01611314210.17221/104/2024-CJGPBcjg-202501-0003High levels of type II Fusarium head blight resistance conferred in wheat by combining wheat gene Fhb1 with Lophopyrum elongatum gene Fhb7The2Jan Dvorak0Karin R. Deal1Patrick E. McGuire2Emily J. Conley3James A. Anderson4George Fedak5Julia A. Malvick6Han Chen7Hans-Georg Müller8Department of Plant Sciences, University of California, Davis, USADepartment of Plant Sciences, University of California, Davis, USADepartment of Plant Sciences, University of California, Davis, USADepartment of Agronomy and Plant Genetics, University of Minnesota, St. Paul, USADepartment of Agronomy and Plant Genetics, University of Minnesota, St. Paul, USAOttawa Research and Development Center, Agriculture and Agri-Food Canada, Ottawa, CanadaVeterinary Genetics Laboratory, University of California, Davis, USADepartment of Statistics, University of California, Davis, USADepartment of Statistics, University of California, Davis, USAWheat Fusarium head blight (FHB) leads to losses of grain yield and quality. Ingestion of diseased grain is detrimental to human health due to the mycotoxins present in the grain. Developing resistant cultivars for environments where FHB is prevalent is therefore an important breeding objective. One of the most effective wheat genes conferring type II resistance to FHB is Fhb1, originally discovered in the Chinese cultivar (cv) Sumai 3. Another excellent FHB resistance gene is Fhb7 located on the long arm of Lophopyrum elongatum chromosome 7E. Several alleles of Fhb7 have been identified. Allele Fhb7The2 was found in disomic substitution lines 7E(7A), 7E(7B) and 7E(7D) derived from amphiploid AgCS. The amphiploid was produced from a hybrid Triticum aestivum cv Chinese Spring × L. elongatum. To find if combining Fhb7The2 with Fhb1 confers higher resistance in wheat than single genes, an introgression line derived from AgCS and possessing Fhb7The2 was recurrently backcrossed to bread wheat cv Rollag and MN-Washburn possessing Fhb1. Experimental lines possessing both Fhb7The2 and Fhb1 were developed and validated cytogenetically and with the L. elongatum genome-wide Sequenom SNP MassARRAY. Spikes of these lines, parental cv Rollag and MN-Washburn, and those of disomic addition line 7E possessing Fhb7The2 plus controls were inoculated with Fusarium in a twice-replicated trial in controlled greenhouse environmental conditions. FHB infection rates were significantly lower in lines combining Fhb7The2 with Fhb1 than in materials with Fhb7The2 or Fhb1 alone.https://cjgpb.agriculturejournals.cz/artkey/cjg-202501-0003_high-levels-of-type-ii-fusarium-head-blight-resistance-conferred-in-wheat-by-combining-wheat-gene-fhb1-with-lop.phpaneuploidybread wheatdisomic additionditelosomic additionintrogressionmeiotic stabilityrobertsonian translocationsequenomsnpthinopyrum |
| spellingShingle | Jan Dvorak Karin R. Deal Patrick E. McGuire Emily J. Conley James A. Anderson George Fedak Julia A. Malvick Han Chen Hans-Georg Müller High levels of type II Fusarium head blight resistance conferred in wheat by combining wheat gene Fhb1 with Lophopyrum elongatum gene Fhb7The2 Czech Journal of Genetics and Plant Breeding aneuploidy bread wheat disomic addition ditelosomic addition introgression meiotic stability robertsonian translocation sequenom snp thinopyrum |
| title | High levels of type II Fusarium head blight resistance conferred in wheat by combining wheat gene Fhb1 with Lophopyrum elongatum gene Fhb7The2 |
| title_full | High levels of type II Fusarium head blight resistance conferred in wheat by combining wheat gene Fhb1 with Lophopyrum elongatum gene Fhb7The2 |
| title_fullStr | High levels of type II Fusarium head blight resistance conferred in wheat by combining wheat gene Fhb1 with Lophopyrum elongatum gene Fhb7The2 |
| title_full_unstemmed | High levels of type II Fusarium head blight resistance conferred in wheat by combining wheat gene Fhb1 with Lophopyrum elongatum gene Fhb7The2 |
| title_short | High levels of type II Fusarium head blight resistance conferred in wheat by combining wheat gene Fhb1 with Lophopyrum elongatum gene Fhb7The2 |
| title_sort | high levels of type ii fusarium head blight resistance conferred in wheat by combining wheat gene fhb1 with lophopyrum elongatum gene fhb7the2 |
| topic | aneuploidy bread wheat disomic addition ditelosomic addition introgression meiotic stability robertsonian translocation sequenom snp thinopyrum |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-202501-0003_high-levels-of-type-ii-fusarium-head-blight-resistance-conferred-in-wheat-by-combining-wheat-gene-fhb1-with-lop.php |
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