Genome-wide identification, classification, and expression profiling of LAC gene family in sesame
Abstract Background Laccases (LACs) are vital plant growth and development enzymes, participating in lignin biopolymerization and responding to stress. However, the role of LAC genes in plant development as well as stress tolerance, is still not well understood, particularly in sesame (Sesamum indic...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-12-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-024-05982-w |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846101330733563904 |
|---|---|
| author | Jianglong Zhou Fengduo Hu Muez Berhe Rong Zhou Donghua Li Huan Li Li Yang Ting Zhou Yanxin Zhang Linhai Wang Jun You |
| author_facet | Jianglong Zhou Fengduo Hu Muez Berhe Rong Zhou Donghua Li Huan Li Li Yang Ting Zhou Yanxin Zhang Linhai Wang Jun You |
| author_sort | Jianglong Zhou |
| collection | DOAJ |
| description | Abstract Background Laccases (LACs) are vital plant growth and development enzymes, participating in lignin biopolymerization and responding to stress. However, the role of LAC genes in plant development as well as stress tolerance, is still not well understood, particularly in sesame (Sesamum indicum L.), an important oilseed crop. Results In this study, 51 sesame LAC genes (SiLACs) were identified, which were unevenly distributed across different chromosomes. The phylogeny of Arabidopsis LAC (AtLACs) subdivided the SiLAC proteins into seven subgroups (Groups I-VII), of which Group VII contained only sesame LACs. Within the same subgroup, SiLACs exhibit comparable structures and conserved motifs. The promoter region of SiLACs harbors various cis-acting elements that are related to plant growth, phytohormones, and stress responses. Most SiLACs were expressed in the roots and stems, whereas some were expressed specifically in flowers or seeds. RNA-seq analysis revealed that 19 SiLACs exhibited down-regulation and three showed up-regulation in response to drought stress, while 15 SiLACs were down-regulated and four up-regulated under salt stress. Additionally, qRT-PCR analysis showcased that certain SiLAC expression was significantly upregulated as a result of osmotic and salt stress. SiLAC5 and SiLAC17 exhibited the most significant changes in expression under osmotic and salt stresses, indicating that they may serve as potential targets for improving sesame resistance to various stresses. Conclusions Our study offers a thorough comprehension of LAC gene structure, classification, evolution, and abiotic stress response in sesame plants. Furthermore, we provide indispensable genetic resources for sesame functional characterization to enhance its tolerance to various abiotic stresses. |
| format | Article |
| id | doaj-art-4a49fad94c9c4f5d95cc03ee10bea13c |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-4a49fad94c9c4f5d95cc03ee10bea13c2024-12-29T12:15:00ZengBMCBMC Plant Biology1471-22292024-12-0124111510.1186/s12870-024-05982-wGenome-wide identification, classification, and expression profiling of LAC gene family in sesameJianglong Zhou0Fengduo Hu1Muez Berhe2Rong Zhou3Donghua Li4Huan Li5Li Yang6Ting Zhou7Yanxin Zhang8Linhai Wang9Jun You10Key Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Oil Crops of the Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute of the Chinese Academy of Agricultural SciencesAbstract Background Laccases (LACs) are vital plant growth and development enzymes, participating in lignin biopolymerization and responding to stress. However, the role of LAC genes in plant development as well as stress tolerance, is still not well understood, particularly in sesame (Sesamum indicum L.), an important oilseed crop. Results In this study, 51 sesame LAC genes (SiLACs) were identified, which were unevenly distributed across different chromosomes. The phylogeny of Arabidopsis LAC (AtLACs) subdivided the SiLAC proteins into seven subgroups (Groups I-VII), of which Group VII contained only sesame LACs. Within the same subgroup, SiLACs exhibit comparable structures and conserved motifs. The promoter region of SiLACs harbors various cis-acting elements that are related to plant growth, phytohormones, and stress responses. Most SiLACs were expressed in the roots and stems, whereas some were expressed specifically in flowers or seeds. RNA-seq analysis revealed that 19 SiLACs exhibited down-regulation and three showed up-regulation in response to drought stress, while 15 SiLACs were down-regulated and four up-regulated under salt stress. Additionally, qRT-PCR analysis showcased that certain SiLAC expression was significantly upregulated as a result of osmotic and salt stress. SiLAC5 and SiLAC17 exhibited the most significant changes in expression under osmotic and salt stresses, indicating that they may serve as potential targets for improving sesame resistance to various stresses. Conclusions Our study offers a thorough comprehension of LAC gene structure, classification, evolution, and abiotic stress response in sesame plants. Furthermore, we provide indispensable genetic resources for sesame functional characterization to enhance its tolerance to various abiotic stresses.https://doi.org/10.1186/s12870-024-05982-wLAC gene familySesamum indicumGene expressionAbiotic stress |
| spellingShingle | Jianglong Zhou Fengduo Hu Muez Berhe Rong Zhou Donghua Li Huan Li Li Yang Ting Zhou Yanxin Zhang Linhai Wang Jun You Genome-wide identification, classification, and expression profiling of LAC gene family in sesame BMC Plant Biology LAC gene family Sesamum indicum Gene expression Abiotic stress |
| title | Genome-wide identification, classification, and expression profiling of LAC gene family in sesame |
| title_full | Genome-wide identification, classification, and expression profiling of LAC gene family in sesame |
| title_fullStr | Genome-wide identification, classification, and expression profiling of LAC gene family in sesame |
| title_full_unstemmed | Genome-wide identification, classification, and expression profiling of LAC gene family in sesame |
| title_short | Genome-wide identification, classification, and expression profiling of LAC gene family in sesame |
| title_sort | genome wide identification classification and expression profiling of lac gene family in sesame |
| topic | LAC gene family Sesamum indicum Gene expression Abiotic stress |
| url | https://doi.org/10.1186/s12870-024-05982-w |
| work_keys_str_mv | AT jianglongzhou genomewideidentificationclassificationandexpressionprofilingoflacgenefamilyinsesame AT fengduohu genomewideidentificationclassificationandexpressionprofilingoflacgenefamilyinsesame AT muezberhe genomewideidentificationclassificationandexpressionprofilingoflacgenefamilyinsesame AT rongzhou genomewideidentificationclassificationandexpressionprofilingoflacgenefamilyinsesame AT donghuali genomewideidentificationclassificationandexpressionprofilingoflacgenefamilyinsesame AT huanli genomewideidentificationclassificationandexpressionprofilingoflacgenefamilyinsesame AT liyang genomewideidentificationclassificationandexpressionprofilingoflacgenefamilyinsesame AT tingzhou genomewideidentificationclassificationandexpressionprofilingoflacgenefamilyinsesame AT yanxinzhang genomewideidentificationclassificationandexpressionprofilingoflacgenefamilyinsesame AT linhaiwang genomewideidentificationclassificationandexpressionprofilingoflacgenefamilyinsesame AT junyou genomewideidentificationclassificationandexpressionprofilingoflacgenefamilyinsesame |