Integrated Metabolome and Transcriptome Analyses Provides Insights into Ovule Abortion in <i>Camellia oleifera</i>

<i>Camellia oleifera</i> is a unique woody edible oil tree species in China, and the ovule development affects the yield of seeds. This study selected three different types of <i>C. oleifera</i> clones and used LC-MS, RNA-seq, and other techniques to compare the endogenous ho...

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Main Authors: Yayan Zhu, Jiajuan Xu, Gang Wang, Feng Xiao, Minggang Zhang, Qinmeng Zeng, Jie Xu
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Plants
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Online Access:https://www.mdpi.com/2223-7747/14/4/613
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Summary:<i>Camellia oleifera</i> is a unique woody edible oil tree species in China, and the ovule development affects the yield of seeds. This study selected three different types of <i>C. oleifera</i> clones and used LC-MS, RNA-seq, and other techniques to compare the endogenous hormone contents, gene expression levels, and metabolite changes between normal and aborted ovules. The results showed that high levels of ABA, JA, and SA may lead to the phenotype of ovule abortion. A total of 270 differential metabolites were identified in the metabolome, with L-methionine, citrulline, L-tryptophan, L-phenylalanine, and indolepyruvate being downregulated to varying degrees in the aborted ovules. Genes involved in plant hormone synthesis and response, such as <i>GH3.1</i>, <i>IAA14</i>, <i>PIN1</i>, <i>AUX22</i>, <i>ARF1_2</i>, <i>BZR1_2</i>, <i>GA2ox</i>, <i>ERFC3</i>, <i>ABF2</i>, and <i>PYL8</i>, responded to ovule development. This study elucidates the physiological, metabolic, and transcriptional responses to ovule abortion, providing a theoretical basis for understanding ovule development and yield regulation in <i>C. oleifera</i>.
ISSN:2223-7747