Development of an aqueous kinetic spectrophotometric method for the analysis of lacosamide optimized through Taguchi and Box-Behnken design; A comparative green assessment
A green and sustainable redox reaction for the quantitative determination of lacosamide is developed using kinetic spectrophotometry. The method development involves the reaction between lacosamide and an aqueous solution of alkaline KMnO4, which form a product of a bluish-green colour at room tempe...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
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| Series: | Green Analytical Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772577424001071 |
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| Summary: | A green and sustainable redox reaction for the quantitative determination of lacosamide is developed using kinetic spectrophotometry. The method development involves the reaction between lacosamide and an aqueous solution of alkaline KMnO4, which form a product of a bluish-green colour at room temperature that was studied on a UV–visible spectrophotometer at 610 nm. Various factors influence the colour's development and its stability. The Box-Behnken design was used to optimize the reaction conditions as well as to achieve the desirability function (drug concentration 42 µg mL−1, KMnO4 concentration 163 µg mL−1at 1443.8 s) to record the maximum absorbance. The Box Behnken Design method was compared with the Taguchi method (drug concentration = 60 µg mL−1, KMnO4 concentration = 200 µg mL−1 at 1050 s), and a similar absorbance was obtained along with a comparable S/N ratio with significant controlling factors. The initial rate, fixed time, fixed absorbance, order of reaction and rate constant methods were studied but the fixed time and initial rate of reaction methods were found more appropriate. The calibration plot is linear from 5 µg mL−1 to 60 µg mL−1 with a correlation coefficient value of 0.9994. Detection limit (LOD) and quantification limit (LOQ) were found 1.31 µg mL−1 and 3.9 µg mL−1, respectively. Using AGREE and analytical eco-scale tools the developed method's greenness was evaluated according to its guidelines and found to be up to the par. The applicability of the proposed green analytical method is used to determine lacosamide in its formulations. |
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| ISSN: | 2772-5774 |