Hydrogen-Rich Saline Attenuates Acute Renal Injury in Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting ROS and NF-κB Pathway
Hydrogen (H2), a new antioxidant, was reported to reduce •OH and ONOO− selectively and inhibit certain proinflammatory mediators to product, without disturbing metabolic redox reactions or ROS involved in cell signaling. We herein aim to explore its protective effects on acute renal injury in sodium...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/685043 |
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| author | Qiao Shi Kang-Shu Liao Kai-Liang Zhao Wei-Xing Wang Teng Zuo Wen-Hong Deng Chen Chen Jia Yu Wen-Yi Guo Xiao-Bo He Ablikim Abliz Peng Wang Liang Zhao |
| author_facet | Qiao Shi Kang-Shu Liao Kai-Liang Zhao Wei-Xing Wang Teng Zuo Wen-Hong Deng Chen Chen Jia Yu Wen-Yi Guo Xiao-Bo He Ablikim Abliz Peng Wang Liang Zhao |
| author_sort | Qiao Shi |
| collection | DOAJ |
| description | Hydrogen (H2), a new antioxidant, was reported to reduce •OH and ONOO− selectively and inhibit certain proinflammatory mediators to product, without disturbing metabolic redox reactions or ROS involved in cell signaling. We herein aim to explore its protective effects on acute renal injury in sodium taurocholate-induced acute pancreatitis and its possible mechanisms. Rats were injected with hydrogen-rich saline (HRS group) or normal saline (SO and SAP group) through tail intravenously (6 mL/kg) and compensated subcutaneously (20 mL/kg) after successful modeling. Results showed that hydrogen-rich saline attenuated the following: (1) serum Cr and BUN, (2) pancreatic and renal pathological injuries, (3) renal MDA, (4) renal MPO, (5) serum IL-1β, IL-6, and renal TNF-α, HMGB1, and (6) tyrosine nitration, IκB degradation, and NF-κB activation in renal tissues. In addition, it increased the level of IL-10 and SOD activity in renal tissues. These results proved that hydrogen-rich saline attenuates acute renal injury in sodium taurocholate-induced acute pancreatitis, presumably because of its detoxification activity against excessive ROS, and inhibits the activation of NF-κB by affecting IκB nitration and degradation. Our findings highlight the potential value of hydrogen-rich saline as a new therapeutic method on acute renal injury in severe acute pancreatitis clinically. |
| format | Article |
| id | doaj-art-4a059b9bca624a7eb25e58b87412a46e |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-4a059b9bca624a7eb25e58b87412a46e2025-02-03T05:47:08ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/685043685043Hydrogen-Rich Saline Attenuates Acute Renal Injury in Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting ROS and NF-κB PathwayQiao Shi0Kang-Shu Liao1Kai-Liang Zhao2Wei-Xing Wang3Teng Zuo4Wen-Hong Deng5Chen Chen6Jia Yu7Wen-Yi Guo8Xiao-Bo He9Ablikim Abliz10Peng Wang11Liang Zhao12Department of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of Hepatobiliary Surgery, Central Hospital of Enshi Autonomous Prefecture, Enshi, Hubei 445000, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaHydrogen (H2), a new antioxidant, was reported to reduce •OH and ONOO− selectively and inhibit certain proinflammatory mediators to product, without disturbing metabolic redox reactions or ROS involved in cell signaling. We herein aim to explore its protective effects on acute renal injury in sodium taurocholate-induced acute pancreatitis and its possible mechanisms. Rats were injected with hydrogen-rich saline (HRS group) or normal saline (SO and SAP group) through tail intravenously (6 mL/kg) and compensated subcutaneously (20 mL/kg) after successful modeling. Results showed that hydrogen-rich saline attenuated the following: (1) serum Cr and BUN, (2) pancreatic and renal pathological injuries, (3) renal MDA, (4) renal MPO, (5) serum IL-1β, IL-6, and renal TNF-α, HMGB1, and (6) tyrosine nitration, IκB degradation, and NF-κB activation in renal tissues. In addition, it increased the level of IL-10 and SOD activity in renal tissues. These results proved that hydrogen-rich saline attenuates acute renal injury in sodium taurocholate-induced acute pancreatitis, presumably because of its detoxification activity against excessive ROS, and inhibits the activation of NF-κB by affecting IκB nitration and degradation. Our findings highlight the potential value of hydrogen-rich saline as a new therapeutic method on acute renal injury in severe acute pancreatitis clinically.http://dx.doi.org/10.1155/2015/685043 |
| spellingShingle | Qiao Shi Kang-Shu Liao Kai-Liang Zhao Wei-Xing Wang Teng Zuo Wen-Hong Deng Chen Chen Jia Yu Wen-Yi Guo Xiao-Bo He Ablikim Abliz Peng Wang Liang Zhao Hydrogen-Rich Saline Attenuates Acute Renal Injury in Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting ROS and NF-κB Pathway Mediators of Inflammation |
| title | Hydrogen-Rich Saline Attenuates Acute Renal Injury in Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting ROS and NF-κB Pathway |
| title_full | Hydrogen-Rich Saline Attenuates Acute Renal Injury in Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting ROS and NF-κB Pathway |
| title_fullStr | Hydrogen-Rich Saline Attenuates Acute Renal Injury in Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting ROS and NF-κB Pathway |
| title_full_unstemmed | Hydrogen-Rich Saline Attenuates Acute Renal Injury in Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting ROS and NF-κB Pathway |
| title_short | Hydrogen-Rich Saline Attenuates Acute Renal Injury in Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting ROS and NF-κB Pathway |
| title_sort | hydrogen rich saline attenuates acute renal injury in sodium taurocholate induced severe acute pancreatitis by inhibiting ros and nf κb pathway |
| url | http://dx.doi.org/10.1155/2015/685043 |
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