miRNA-328-3p regulates ZO-1 expression and inhibits PEDV proliferation via the PLC-β1-PKC pathway.
Porcine epidemic diarrhea virus (PEDV) is a significant pathogen affecting swine, causing severe economic losses worldwide. This study explores the regulatory role of miRNA-328-3p to ZO-1 expression and its impact on PEDV proliferation via the PLC-β1-PKC pathway in IPEC-J2 cells. We found that miRNA...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0316074 |
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| author | Han Zhao Jiayu Zhang Dengliang Li Zhanding Cui Jiuyuan Liu Di Bao Yiming Wei Xinyue Zhang Zhimin Wu Tianqi Zhang Kai Wang Shushuai Yi Wei Lian Guixue Hu |
| author_facet | Han Zhao Jiayu Zhang Dengliang Li Zhanding Cui Jiuyuan Liu Di Bao Yiming Wei Xinyue Zhang Zhimin Wu Tianqi Zhang Kai Wang Shushuai Yi Wei Lian Guixue Hu |
| author_sort | Han Zhao |
| collection | DOAJ |
| description | Porcine epidemic diarrhea virus (PEDV) is a significant pathogen affecting swine, causing severe economic losses worldwide. This study explores the regulatory role of miRNA-328-3p to ZO-1 expression and its impact on PEDV proliferation via the PLC-β1-PKC pathway in IPEC-J2 cells. We found that miRNA-328-3p can target ZO-1, influencing its expression and subsequently affecting the integrity of tight junctions in the cells. Overexpression of PLC-β1, combined with miRNA-328-3p silencing, enhanced ZO-1 expression, while PLC-β1 knockdown combined with miRNA-328-3p overexpression inhibited ZO-1 expression. Furthermore, PLC-β1 overexpression increased both viral genome expression and PEDV titers, whereas its silencing had the opposite effect. Notably, our data indicated a negative correlation between PLC-β1 and PKC expression, and PKC silencing attenuated the upregulatory effect of PLC-β1 on ZO-1. These findings suggest that PLC-β1 modulates ZO-1 expression through the PKC pathway, providing new insights into the molecular mechanisms of PEDV infection and potential therapeutic targets. |
| format | Article |
| id | doaj-art-49d86ae603f64133a529f42791c2bc7d |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-49d86ae603f64133a529f42791c2bc7d2025-01-08T05:31:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01201e031607410.1371/journal.pone.0316074miRNA-328-3p regulates ZO-1 expression and inhibits PEDV proliferation via the PLC-β1-PKC pathway.Han ZhaoJiayu ZhangDengliang LiZhanding CuiJiuyuan LiuDi BaoYiming WeiXinyue ZhangZhimin WuTianqi ZhangKai WangShushuai YiWei LianGuixue HuPorcine epidemic diarrhea virus (PEDV) is a significant pathogen affecting swine, causing severe economic losses worldwide. This study explores the regulatory role of miRNA-328-3p to ZO-1 expression and its impact on PEDV proliferation via the PLC-β1-PKC pathway in IPEC-J2 cells. We found that miRNA-328-3p can target ZO-1, influencing its expression and subsequently affecting the integrity of tight junctions in the cells. Overexpression of PLC-β1, combined with miRNA-328-3p silencing, enhanced ZO-1 expression, while PLC-β1 knockdown combined with miRNA-328-3p overexpression inhibited ZO-1 expression. Furthermore, PLC-β1 overexpression increased both viral genome expression and PEDV titers, whereas its silencing had the opposite effect. Notably, our data indicated a negative correlation between PLC-β1 and PKC expression, and PKC silencing attenuated the upregulatory effect of PLC-β1 on ZO-1. These findings suggest that PLC-β1 modulates ZO-1 expression through the PKC pathway, providing new insights into the molecular mechanisms of PEDV infection and potential therapeutic targets.https://doi.org/10.1371/journal.pone.0316074 |
| spellingShingle | Han Zhao Jiayu Zhang Dengliang Li Zhanding Cui Jiuyuan Liu Di Bao Yiming Wei Xinyue Zhang Zhimin Wu Tianqi Zhang Kai Wang Shushuai Yi Wei Lian Guixue Hu miRNA-328-3p regulates ZO-1 expression and inhibits PEDV proliferation via the PLC-β1-PKC pathway. PLoS ONE |
| title | miRNA-328-3p regulates ZO-1 expression and inhibits PEDV proliferation via the PLC-β1-PKC pathway. |
| title_full | miRNA-328-3p regulates ZO-1 expression and inhibits PEDV proliferation via the PLC-β1-PKC pathway. |
| title_fullStr | miRNA-328-3p regulates ZO-1 expression and inhibits PEDV proliferation via the PLC-β1-PKC pathway. |
| title_full_unstemmed | miRNA-328-3p regulates ZO-1 expression and inhibits PEDV proliferation via the PLC-β1-PKC pathway. |
| title_short | miRNA-328-3p regulates ZO-1 expression and inhibits PEDV proliferation via the PLC-β1-PKC pathway. |
| title_sort | mirna 328 3p regulates zo 1 expression and inhibits pedv proliferation via the plc β1 pkc pathway |
| url | https://doi.org/10.1371/journal.pone.0316074 |
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